Abstract
Thirty cytomegalovirus cell culture-positive samples were tested by the SHARP Signal System. Twenty-seven specimens (100% agreement) were identified by both methods. The SHARP Signal System is rapid (4 h), easy to perform, and potentially adaptable to automation.
Full Text
The Full Text of this article is available as a PDF (149.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Delgado R., Lumbreras C., Alba C., Pedraza M. A., Otero J. R., Gómez R., Moreno E., Noriega A. R., Payá C. V. Low predictive value of polymerase chain reaction for diagnosis of cytomegalovirus disease in liver transplant recipients. J Clin Microbiol. 1992 Jul;30(7):1876–1878. doi: 10.1128/jcm.30.7.1876-1878.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Demmler G. J., Buffone G. J., Schimbor C. M., May R. A. Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification. J Infect Dis. 1988 Dec;158(6):1177–1184. doi: 10.1093/infdis/158.6.1177. [DOI] [PubMed] [Google Scholar]
- Ellis M. E., Spence D., Bouchama A., Antonius J., Bazarbashi M., Khougeer F., De Vol E. B. Open lung biopsy provides a higher and more specific diagnostic yield compared to broncho-alveolar lavage in immunocompromised patients. Fungal Study Group. Scand J Infect Dis. 1995;27(2):157–162. doi: 10.3109/00365549509018998. [DOI] [PubMed] [Google Scholar]
- Espy M. J., Patel R., Paya C. V., Smith T. F. Comparison of three methods for extraction of viral nucleic acids from blood cultures. J Clin Microbiol. 1995 Jan;33(1):41–44. doi: 10.1128/jcm.33.1.41-44.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Espy M. J., Smith T. F., Persing D. H. Dependence of polymerase chain reaction product inactivation protocols on amplicon length and sequence composition. J Clin Microbiol. 1993 Sep;31(9):2361–2365. doi: 10.1128/jcm.31.9.2361-2365.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyers J. D., Ljungman P., Fisher L. D. Cytomegalovirus excretion as a predictor of cytomegalovirus disease after marrow transplantation: importance of cytomegalovirus viremia. J Infect Dis. 1990 Aug;162(2):373–380. doi: 10.1093/infdis/162.2.373. [DOI] [PubMed] [Google Scholar]
- Myerson D., Lingenfelter P. A., Gleaves C. A., Meyers J. D., Bowden R. A. Diagnosis of cytomegalovirus pneumonia by the polymerase chain reaction with archived frozen lung tissue and bronchoalveolar lavage fluid. Am J Clin Pathol. 1993 Oct;100(4):407–413. doi: 10.1093/ajcp/100.4.407. [DOI] [PubMed] [Google Scholar]
- Patel R., Smith T. F., Espy M., Wiesner R. H., Krom R. A., Portela D., Paya C. V. Detection of cytomegalovirus DNA in sera of liver transplant recipients. J Clin Microbiol. 1994 Jun;32(6):1431–1434. doi: 10.1128/jcm.32.6.1431-1434.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shibata D., Martin W. J., Appleman M. D., Causey D. M., Leedom J. M., Arnheim N. Detection of cytomegalovirus DNA in peripheral blood of patients infected with human immunodeficiency virus. J Infect Dis. 1988 Dec;158(6):1185–1192. doi: 10.1093/infdis/158.6.1185. [DOI] [PubMed] [Google Scholar]
- Storch G. A., Buller R. S., Bailey T. C., Ettinger N. A., Langlois T., Gaudreault-Keener M., Welby P. L. Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients. J Clin Microbiol. 1994 Apr;32(4):997–1003. doi: 10.1128/jcm.32.4.997-1003.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Valentine-Thon E. Evaluation of SHARP signal system for enzymatic detection of amplified hepatitis B virus DNA. J Clin Microbiol. 1995 Feb;33(2):477–480. doi: 10.1128/jcm.33.2.477-480.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolf D. G., Spector S. A. Early diagnosis of human cytomegalovirus disease in transplant recipients by DNA amplification in plasma. Transplantation. 1993 Aug;56(2):330–334. doi: 10.1097/00007890-199308000-00014. [DOI] [PubMed] [Google Scholar]