Abstract
A combination of two PCR systems which amplify human immunodeficiency virus type 1 (HIV-1) gag and pol DNA was used to elucidate the HIV-1 infection status of a group of individuals screening positive for anti-HIV but having indeterminate Western blot (immunoblot) profiles. The results obtained suggest PCR could be used to resolve such cases.
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Selected References
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- Albert J., Fenyö E. M. Simple, sensitive, and specific detection of human immunodeficiency virus type 1 in clinical specimens by polymerase chain reaction with nested primers. J Clin Microbiol. 1990 Jul;28(7):1560–1564. doi: 10.1128/jcm.28.7.1560-1564.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bettaieb A., Fromont P., Louache F., Oksenhendler E., Vainchenker W., Duédari N., Bierling P. Presence of cross-reactive antibody between human immunodeficiency virus (HIV) and platelet glycoproteins in HIV-related immune thrombocytopenic purpura. Blood. 1992 Jul 1;80(1):162–169. [PubMed] [Google Scholar]
- Bieniasz P. D., Ariyoshi K., Bourelly M. A., Bloor S., Foxall R. B., Harwood E. C., Weber J. N. Variable relationship between proviral DNA load and infectious virus titre in the peripheral blood mononuclear cells of HIV-1-infected individuals. AIDS. 1993 Jun;7(6):803–806. doi: 10.1097/00002030-199306000-00007. [DOI] [PubMed] [Google Scholar]
- Drabick J. J., Baker J. R., Jr HLA antigen contamination of commercial Western blot strips for detecting human immunodeficiency virus. J Infect Dis. 1989 Feb;159(2):357–358. doi: 10.1093/infdis/159.2.357. [DOI] [PubMed] [Google Scholar]
- Farzadegan H., Vlahov D., Solomon L., Muñoz A., Astemborski J., Taylor E., Burnley A., Nelson K. E. Detection of human immunodeficiency virus type 1 infection by polymerase chain reaction in a cohort of seronegative intravenous drug users. J Infect Dis. 1993 Aug;168(2):327–331. doi: 10.1093/infdis/168.2.327. [DOI] [PubMed] [Google Scholar]
- Hohmann A. W., Booth K., Peters V., Gordon D. L., Comacchio R. M. Common epitope on HIV p24 and human platelets. Lancet. 1993 Nov 20;342(8882):1274–1275. doi: 10.1016/0140-6736(93)92363-x. [DOI] [PubMed] [Google Scholar]
- Hsia J. False-positive ELISA for human immunodeficiency virus after influenza vaccination. J Infect Dis. 1993 Apr;167(4):989–990. doi: 10.1093/infdis/167.4.989. [DOI] [PubMed] [Google Scholar]
- Kemp D. J., Churchill M. J., Smith D. B., Biggs B. A., Foote S. J., Peterson M. G., Samaras N., Deacon N. J., Doherty R. Simplified colorimetric analysis of polymerase chain reactions: detection of HIV sequences in AIDS patients. Gene. 1990 Oct 15;94(2):223–228. doi: 10.1016/0378-1119(90)90391-4. [DOI] [PubMed] [Google Scholar]
- Lee D. A., Eby W. C., Molinaro G. A. HIV false positivity after hepatitis B vaccination. Lancet. 1992 Apr 25;339(8800):1060–1060. doi: 10.1016/0140-6736(92)90586-r. [DOI] [PubMed] [Google Scholar]
- Léonard G., Chaput A., Courgnaud V., Sangaré A., Denis F., Brechot C. Characterization of dual HIV-1 and HIV-2 serological profiles by polymerase chain reaction. AIDS. 1993 Sep;7(9):1185–1189. doi: 10.1097/00002030-199309000-00006. [DOI] [PubMed] [Google Scholar]
- Nkengasong J. N., Peeters M., vanden Haesevelde M., Musi S. S., Willems B., Ndumbe P. M., Delaporte E., Perret J. L., Piot P., van den Groen G. Antigenic evidence of the presence of the aberrant HIV-1ant70 virus in Cameroon and Gabon. AIDS. 1993 Nov;7(11):1536–1538. [PubMed] [Google Scholar]
- Rogers M. F., Ou C. Y., Rayfield M., Thomas P. A., Schoenbaum E. E., Abrams E., Krasinski K., Selwyn P. A., Moore J., Kaul A. Use of the polymerase chain reaction for early detection of the proviral sequences of human immunodeficiency virus in infants born to seropositive mothers. New York City Collaborative Study of Maternal HIV Transmission and Montefiore Medical Center HIV Perinatal Transmission Study Group. N Engl J Med. 1989 Jun 22;320(25):1649–1654. doi: 10.1056/NEJM198906223202503. [DOI] [PubMed] [Google Scholar]
- Wolinsky S. M., Rinaldo C. R., Kwok S., Sninsky J. J., Gupta P., Imagawa D., Farzadegan H., Jacobson L. P., Grovit K. S., Lee M. H. Human immunodeficiency virus type 1 (HIV-1) infection a median of 18 months before a diagnostic western blot. Evidence from a cohort of homosexual men. Ann Intern Med. 1989 Dec 15;111(12):961–972. doi: 10.7326/0003-4819-111-12-961. [DOI] [PubMed] [Google Scholar]