Abstract
Five hundred five blood samples for culture were processed in the Isolator lysis-centrifugation system and were then inoculated into a Mycobacteria Growth Indicator Tube (MGIT) and onto a Lowenstein-Jensen (L-J) slant. Forty-nine isolates of Mycobacterium avium complex and three isolates of Mycobacterium tuberculosis were recovered from 50 of the blood culture specimens. Forty-five isolates from 43 specimens were recovered in the MGIT, with a mean time to detection of 21 days. Forty-one isolates from 40 specimens were recovered in the L-J slants, and the mean time to detection was 36 days. Nine specimens were positive in the MGIT alone, while seven specimens were positive only in L-J medium.
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Selected References
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- Agy M. B., Wallis C. K., Plorde J. J., Carlson L. C., Coyle M. B. Evaluation of four mycobacterial blood culture media: BACTEC 13A, Isolator/BACTEC 12B, Isolator/Middlebrook agar, and a biphasic medium. Diagn Microbiol Infect Dis. 1989 Jul-Aug;12(4):303–308. doi: 10.1016/0732-8893(89)90094-1. [DOI] [PubMed] [Google Scholar]
- Doern G. V., Westerling J. A. Optimum recovery of Mycobacterium avium complex from blood specimens of human immunodeficiency virus-positive patients by using small volumes of isolator concentrate inoculated into BACTEC 12B bottles. J Clin Microbiol. 1994 Oct;32(10):2576–2577. doi: 10.1128/jcm.32.10.2576-2577.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gill V. J., Park C. H., Stock F., Gosey L. L., Witebsky F. G., Masur H. Use of lysis-centrifugation (isolator) and radiometric (BACTEC) blood culture systems for the detection of mycobacteremia. J Clin Microbiol. 1985 Oct;22(4):543–546. doi: 10.1128/jcm.22.4.543-546.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiehn T. E., Cammarata R. Comparative recoveries of Mycobacterium avium-M. intracellulare from isolator lysis-centrifugation and BACTEC 13A blood culture systems. J Clin Microbiol. 1988 Apr;26(4):760–761. doi: 10.1128/jcm.26.4.760-761.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiehn T. E., Cammarata R. Laboratory diagnosis of mycobacterial infections in patients with acquired immunodeficiency syndrome. J Clin Microbiol. 1986 Nov;24(5):708–711. doi: 10.1128/jcm.24.5.708-711.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Salfinger M., Stool E. W., Piot D., Heifets L. Comparison of three methods for recovery of Mycobacterium avium complex from blood specimens. J Clin Microbiol. 1988 Jun;26(6):1225–1226. doi: 10.1128/jcm.26.6.1225-1226.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wasilauskas B., Morrell R., Jr Inhibitory effect of the Isolator blood culture system on growth of Mycobacterium avium-M. intracellulare in BACTEC 12B bottles. J Clin Microbiol. 1994 Mar;32(3):654–657. doi: 10.1128/jcm.32.3.654-657.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Witebsky F. G., Keiser J. F., Conville P. S., Bryan R., Park C. H., Walker R., Siddiqi S. H. Comparison of BACTEC 13A medium and Du Pont isolator for detection of mycobacteremia. J Clin Microbiol. 1988 Aug;26(8):1501–1505. doi: 10.1128/jcm.26.8.1501-1505.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]