Abstract
Antigens from Taenia solium cysticerci for the immunodiagnosis of cysticercosis are scarce and difficult to obtain. We studied the reliability of antigens from Taenia crassiceps cysticerci as a substitute for those from T. solium in three diagnostic tests: complement fixation, enzyme-linked immunosorbent assay, and Western blot (immunoblot). Precision rates of the three tests of 93, 99, and 96%, respectively, were found. Cysticerci from T. crassiceps can be easily obtained in large quantities and can be effectively used for the diagnosis of human neurocysticercosis.
Full Text
The Full Text of this article is available as a PDF (149.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baumeister S., Dennis R. D., Kunz J., Wiegandt H., Geyer E. Comparative serological reactivity of Taenia crassiceps, Taenia solium and Taenia saginata metacestode neutral glycolipids to infection serum from Taenia crassiceps-infected mice. Mol Biochem Parasitol. 1992 Jul;53(1-2):53–61. doi: 10.1016/0166-6851(92)90006-6. [DOI] [PubMed] [Google Scholar]
- Garcia E., Sotelo J. A new complement fixation test for the diagnosis of neurocysticercosis in cerebrospinal fluid. J Neurol. 1991 Oct;238(7):379–382. doi: 10.1007/BF00319856. [DOI] [PubMed] [Google Scholar]
- Garcia E., Sotelo J. Storage of cerebrospinal fluid on paper. Lancet. 1989 Oct 28;2(8670):1046–1046. doi: 10.1016/s0140-6736(89)91064-7. [DOI] [PubMed] [Google Scholar]
- Kirchhoff L. V. American trypanosomiasis (Chagas' disease)--a tropical disease now in the United States. N Engl J Med. 1993 Aug 26;329(9):639–644. doi: 10.1056/NEJM199308263290909. [DOI] [PubMed] [Google Scholar]
- Klinker H., Tintelnot K., Joeres R., Müller J., Gross U., Schmidt-Rotte H., Landwehr P., Richter E. Taenia-crassiceps-Infektion bei AIDS. Dtsch Med Wochenschr. 1992 Jan 24;117(4):133–138. doi: 10.1055/s-2008-1062291. [DOI] [PubMed] [Google Scholar]
- Kunz J., Kalinna B., Watschke V., Geyer E. Taenia crassiceps metacestode vesicular fluid antigens shared with the Taenia solium larval stage and reactive with serum antibodies from patients with neurocysticercosis. Zentralbl Bakteriol. 1989 Oct;271(4):510–520. doi: 10.1016/s0934-8840(89)80113-6. [DOI] [PubMed] [Google Scholar]
- Larralde C., Montoya R. M., Sciutto E., Diaz M. L., Govezensky T., Coltorti E. Deciphering western blots of tapeworm antigens (Taenia solium, Echinococcus granulosus, and Taenia crassiceps) reacting with sera from neurocysticercosis and hydatid disease patients. Am J Trop Med Hyg. 1989 Mar;40(3):282–290. doi: 10.4269/ajtmh.1989.40.282. [DOI] [PubMed] [Google Scholar]
- Larralde C., Sotelo J., Montoya R. M., Palencia G., Padilla A., Govezensky T., Diaz M. L., Sciutto E. Immunodiagnosis of human cysticercosis in cerebrospinal fluid. Antigens from murine Taenia crassiceps cysticerci effectively substitute those from porcine Taenia solium. Arch Pathol Lab Med. 1990 Sep;114(9):926–928. [PubMed] [Google Scholar]
- Miller B. L., Staugaitis S. M., Tourtellotte W. W., Shapshak P., Goldberg M., Heiner D., Weil M. Intra-blood-brain barrier IgG synthesis in cerebral cysticercosis. Arch Neurol. 1985 Aug;42(8):782–784. doi: 10.1001/archneur.1985.04210090046013. [DOI] [PubMed] [Google Scholar]
- Ramos-Kuri M., Montoya R. M., Padilla A., Govezensky T., Díaz M. L., Sciutto E., Sotelo J., Larralde C. Immunodiagnosis of neurocysticercosis. Disappointing performance of serology (enzyme-linked immunosorbent assay) in an unbiased sample of neurological patients. Arch Neurol. 1992 Jun;49(6):633–636. doi: 10.1001/archneur.1992.00530300069012. [DOI] [PubMed] [Google Scholar]
- Rosas N., Sotelo J., Nieto D. ELISA in the diagnosis of neurocysticercosis. Arch Neurol. 1986 Apr;43(4):353–356. doi: 10.1001/archneur.1986.00520040039016. [DOI] [PubMed] [Google Scholar]
- Smith J. K., Esch G. W., Kuhn R. E. Growth and development of larval Taenia crassiceps (cestoda). I. Aneuploidy in the anomalous ORF strain. Int J Parasitol. 1972 Jun;2(2):261–263. doi: 10.1016/0020-7519(72)90014-8. [DOI] [PubMed] [Google Scholar]
- Sotelo J., Guerrero V., Rubio F. Neurocysticercosis: a new classification based on active and inactive forms. A study of 753 cases. Arch Intern Med. 1985 Mar;145(3):442–445. [PubMed] [Google Scholar]
- Téllez-Girón E., Ramos M. C., Dufour L., Alvarez P., Montante M. Detection of Cysticercus cellulosae antigens in cerebrospinal fluid by dot enzyme-linked immunosorbent assay (Dot-ELISA) and standard ELISA. Am J Trop Med Hyg. 1987 Jul;37(1):169–173. doi: 10.4269/ajtmh.1987.37.169. [DOI] [PubMed] [Google Scholar]