Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1995 Dec;33(12):3359–3360. doi: 10.1128/jcm.33.12.3359-3360.1995

Can random amplified polymorphic DNA analysis of the 16S-23S spacer region of Mycobacterium tuberculosis differentiate between isolates?

M Glennon, T Smith
PMCID: PMC228711  PMID: 8586740

Full Text

The Full Text of this article is available as a PDF (137.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abed Y., Davin-Regli A., Bollet C., De Micco P. Efficient discrimination of Mycobacterium tuberculosis strains by 16S-23S spacer region-based random amplified polymorphic DNA analysis. J Clin Microbiol. 1995 May;33(5):1418–1420. doi: 10.1128/jcm.33.5.1418-1420.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abed Y., Davin-Regli A., Bollet C., De Micco P. Efficient discrimination of Mycobacterium tuberculosis strains by 16S-23S spacer region-based random amplified polymorphic DNA analysis. J Clin Microbiol. 1995 May;33(5):1418–1420. doi: 10.1128/jcm.33.5.1418-1420.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barry T., Colleran G., Glennon M., Dunican L. K., Gannon F. The 16s/23s ribosomal spacer region as a target for DNA probes to identify eubacteria. PCR Methods Appl. 1991 Aug;1(1):51–56. doi: 10.1101/gr.1.1.51. [DOI] [PubMed] [Google Scholar]
  4. Bercovier H., Kafri O., Sela S. Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome. Biochem Biophys Res Commun. 1986 May 14;136(3):1136–1141. doi: 10.1016/0006-291x(86)90452-3. [DOI] [PubMed] [Google Scholar]
  5. Davin-Regli A., Abed Y., Charrel R. N., Bollet C., de Micco P. Variations in DNA concentrations significantly affect the reproducibility of RAPD fingerprint patterns. Res Microbiol. 1995 Sep;146(7):561–568. doi: 10.1016/0923-2508(96)80562-6. [DOI] [PubMed] [Google Scholar]
  6. Frothingham R., Hills H. G., Wilson K. H. Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex. J Clin Microbiol. 1994 Jul;32(7):1639–1643. doi: 10.1128/jcm.32.7.1639-1643.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Frothingham R., Hills H. G., Wilson K. H. Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex. J Clin Microbiol. 1994 Jul;32(7):1639–1643. doi: 10.1128/jcm.32.7.1639-1643.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frothingham R., Wilson K. H. Molecular phylogeny of the Mycobacterium avium complex demonstrates clinically meaningful divisions. J Infect Dis. 1994 Feb;169(2):305–312. doi: 10.1093/infdis/169.2.305. [DOI] [PubMed] [Google Scholar]
  9. Frothingham R., Wilson K. H. Sequence-based differentiation of strains in the Mycobacterium avium complex. J Bacteriol. 1993 May;175(10):2818–2825. doi: 10.1128/jb.175.10.2818-2825.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Frothingham R., Wilson K. H. Sequence-based differentiation of strains in the Mycobacterium avium complex. J Bacteriol. 1993 May;175(10):2818–2825. doi: 10.1128/jb.175.10.2818-2825.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Glennon M., Smith T., Cormican M., Noone D., Barry T., Maher M., Dawson M., Gilmartin J. J., Gannon F. The ribosomal intergenic spacer region: a target for the PCR based diagnosis of tuberculosis. Tuber Lung Dis. 1994 Oct;75(5):353–360. doi: 10.1016/0962-8479(94)90081-7. [DOI] [PubMed] [Google Scholar]
  12. Hall L. M. Are point mutations or DNA rearrangements responsible for the restriction fragment length polymorphisms that are used to type bacteria? Microbiology. 1994 Jan;140(Pt 1):197–204. doi: 10.1099/13500872-140-1-197. [DOI] [PubMed] [Google Scholar]
  13. Jensen M. A., Webster J. A., Straus N. Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl Environ Microbiol. 1993 Apr;59(4):945–952. doi: 10.1128/aem.59.4.945-952.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ji Y. E., Colston M. J., Cox R. A. Nucleotide sequence and secondary structures of precursor 16S rRNA of slow-growing mycobacteria. Microbiology. 1994 Jan;140(Pt 1):123–132. doi: 10.1099/13500872-140-1-123. [DOI] [PubMed] [Google Scholar]
  15. Kempsell K. E., Ji Y. E., Estrada I. C., Colston M. J., Cox R. A. The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae precursor rRNA. J Gen Microbiol. 1992 Aug;138(Pt 8):1717–1727. doi: 10.1099/00221287-138-8-1717. [DOI] [PubMed] [Google Scholar]
  16. Liesack W., Sela S., Bercovier H., Pitulle C., Stackebrandt E. Complete nucleotide sequence of the Mycobacterium leprae 23 S and 5 S rRNA genes plus flanking regions and their potential in designing diagnostic oligonucleotide probes. FEBS Lett. 1991 Apr 9;281(1-2):114–118. doi: 10.1016/0014-5793(91)80372-a. [DOI] [PubMed] [Google Scholar]
  17. Suzuki Y., Nagata A., Ono Y., Yamada T. Complete nucleotide sequence of the 16S rRNA gene of Mycobacterium bovis BCG. J Bacteriol. 1988 Jun;170(6):2886–2889. doi: 10.1128/jb.170.6.2886-2889.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES