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. 1996 Jan;34(1):71–75. doi: 10.1128/jcm.34.1.71-75.1996

Comparison of infectivities of six tick-derived isolates of Borrelia burgdorferi for rodents and ticks.

C A Peavey 1, R S Lane 1
PMCID: PMC228733  PMID: 8748276

Abstract

The infectivity and dissemination to the skin of six isolates of Borrelia burgdorferi were evaluated by inoculating them into groups of deer mice (Peromyscus maniculatus), hamsters, and Swiss Webster mice. Rodent infection was assayed by culture of ear punch biopsy specimens taken at 4, 8, and 12 weeks postinoculation (p.i.). Spirochetes were detected in biopsy specimens from individuals of all three host species that had been inoculated with four isolates (CA3, CA4, CA7, and CA8). Ear punch biopsy specimens taken from Swiss Webster mice at 12 weeks p.i. yielded an additional reisolate (CA2), even though these animals did not seroconvert. The remaining isolate (CA9) was not recovered from any host. However, two deer mice and all hamsters and Swiss Webster mice inoculated with CA9 seroconverted. All six isolates were of low infectivity to ticks when inoculated intramuscularly into hosts. Only 4 (1.6%) of 250 Ixodes pacificus larvae acquired and transstadially maintained infection from hosts inoculated intramuscularly. Infectivity of three isolates for ticks also was tested in Swiss Webster mice injected intradermally. The mean prevalences of infection in xenodiagnostic ticks fed on these mice at 4 weeks p.i. were 47.9, 1.2, and 2.2% for isolates CA4, CA7, and CA8, respectively. The mean prevalences of infection for ticks fed on the same mice at 12 weeks p.i. were 36.4, 11.8, and 20.4%, respectively. Such differences in the infectivity and rate of dissemination of individual isolates of B. burgdorferi should be considered during studies of reservoir and vector competence.

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Selected References

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  1. Barbour A. G., Heiland R. A., Howe T. R. Heterogeneity of major proteins in Lyme disease borreliae: a molecular analysis of North American and European isolates. J Infect Dis. 1985 Sep;152(3):478–484. doi: 10.1093/infdis/152.3.478. [DOI] [PubMed] [Google Scholar]
  2. Barbour A. G. Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med. 1984 Jul-Aug;57(4):521–525. [PMC free article] [PubMed] [Google Scholar]
  3. Barbour A. G., Schrumpf M. E. Polymorphisms of major surface proteins of Borrelia burgdorferi. Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):83–91. doi: 10.1016/s0176-6724(86)80107-9. [DOI] [PubMed] [Google Scholar]
  4. Barthold S. W., Moody K. D., Beck D. S. Susceptibility of laboratory rats to isolates of Borrelia burgdorferi from different geographic areas. Am J Trop Med Hyg. 1990 Jun;42(6):596–600. doi: 10.4269/ajtmh.1990.42.596. [DOI] [PubMed] [Google Scholar]
  5. Barthold S. W., Moody K. D., Terwilliger G. A., Duray P. H., Jacoby R. O., Steere A. C. Experimental Lyme arthritis in rats infected with Borrelia burgdorferi. J Infect Dis. 1988 Apr;157(4):842–846. doi: 10.1093/infdis/157.4.842. [DOI] [PubMed] [Google Scholar]
  6. Bissett M. L., Hill W. Characterization of Borrelia burgdorferi strains isolated from Ixodes pacificus ticks in California. J Clin Microbiol. 1987 Dec;25(12):2296–2301. doi: 10.1128/jcm.25.12.2296-2301.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brown R. N., Lane R. S. Lyme disease in California: a novel enzootic transmission cycle of Borrelia burgdorferi. Science. 1992 Jun 5;256(5062):1439–1442. doi: 10.1126/science.1604318. [DOI] [PubMed] [Google Scholar]
  8. Brown R. N., Lane R. S. Natural and experimental Borrelia burgdorferi infections in woodrats and deer mice from California. J Wildl Dis. 1994 Jul;30(3):389–398. doi: 10.7589/0090-3558-30.3.389. [DOI] [PubMed] [Google Scholar]
  9. Donahue J. G., Piesman J., Spielman A. Reservoir competence of white-footed mice for Lyme disease spirochetes. Am J Trop Med Hyg. 1987 Jan;36(1):92–96. doi: 10.4269/ajtmh.1987.36.92. [DOI] [PubMed] [Google Scholar]
  10. Johnson R. C., Marek N., Kodner C. Infection of Syrian hamsters with Lyme disease spirochetes. J Clin Microbiol. 1984 Dec;20(6):1099–1101. doi: 10.1128/jcm.20.6.1099-1101.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lane R. S., Brown R. N., Piesman J., Peavey C. A. Vector competence of Ixodes pacificus and Dermacentor occidentalis (Acari: Ixodidae) for various isolates of Lyme disease spirochetes. J Med Entomol. 1994 May;31(3):417–424. doi: 10.1093/jmedent/31.3.417. [DOI] [PubMed] [Google Scholar]
  12. Lane R. S., Brown R. N. Wood rats and kangaroo rats: potential reservoirs of the Lyme disease spirochete in California. J Med Entomol. 1991 May;28(3):299–302. doi: 10.1093/jmedent/28.3.299. [DOI] [PubMed] [Google Scholar]
  13. Lane R. S., Burgdorfer W. Spirochetes in mammals and ticks (Acari: Ixodidae) from a focus of Lyme borreliosis in California. J Wildl Dis. 1988 Jan;24(1):1–9. doi: 10.7589/0090-3558-24.1.1. [DOI] [PubMed] [Google Scholar]
  14. Lane R. S., Pascocello J. A. Antigenic characteristics of Borrelia burgdorferi isolates from ixodid ticks in California. J Clin Microbiol. 1989 Oct;27(10):2344–2349. doi: 10.1128/jcm.27.10.2344-2349.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LeFebvre R. B., Lane R. S., Perng G. C., Brown J. A., Johnson R. C. DNA and protein analyses of tick-derived isolates of Borrelia burgdorferi from California. J Clin Microbiol. 1990 Apr;28(4):700–707. doi: 10.1128/jcm.28.4.700-707.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Masuzawa T., Kurita T., Kawabata H., Yanagihara Y. Relationship between infectivity and OspC expression in Lyme disease Borrelia. FEMS Microbiol Lett. 1994 Nov 1;123(3):319–324. doi: 10.1111/j.1574-6968.1994.tb07242.x. [DOI] [PubMed] [Google Scholar]
  17. Piesman J. Standard system for infecting ticks (Acari: Ixodidae) with the Lyme disease spirochete, Borrelia burgdorferi. J Med Entomol. 1993 Jan;30(1):199–203. doi: 10.1093/jmedent/30.1.199. [DOI] [PubMed] [Google Scholar]
  18. Postic D., Assous M. V., Grimont P. A., Baranton G. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int J Syst Bacteriol. 1994 Oct;44(4):743–752. doi: 10.1099/00207713-44-4-743. [DOI] [PubMed] [Google Scholar]
  19. Schwan T. G., Burgdorfer W., Schrumpf M. E., Karstens R. H. The urinary bladder, a consistent source of Borrelia burgdorferi in experimentally infected white-footed mice (Peromyscus leucopus). J Clin Microbiol. 1988 May;26(5):893–895. doi: 10.1128/jcm.26.5.893-895.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schwan T. G., Piesman J., Golde W. T., Dolan M. C., Rosa P. A. Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2909–2913. doi: 10.1073/pnas.92.7.2909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schwan T. G., Schrumpf M. E., Karstens R. H., Clover J. R., Wong J., Daugherty M., Struthers M., Rosa P. A. Distribution and molecular analysis of Lyme disease spirochetes, Borrelia burgdorferi, isolated from ticks throughout California. J Clin Microbiol. 1993 Dec;31(12):3096–3108. doi: 10.1128/jcm.31.12.3096-3108.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shih C. M., Pollack R. J., Telford S. R., 3rd, Spielman A. Delayed dissemination of Lyme disease spirochetes from the site of deposition in the skin of mice. J Infect Dis. 1992 Oct;166(4):827–831. doi: 10.1093/infdis/166.4.827. [DOI] [PubMed] [Google Scholar]
  23. Shih C. M., Telford S. R., 3rd, Pollack R. J., Spielman A. Rapid dissemination by the agent of Lyme disease in hosts that permit fulminating infection. Infect Immun. 1993 Jun;61(6):2396–2399. doi: 10.1128/iai.61.6.2396-2399.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sinsky R. J., Piesman J. Ear punch biopsy method for detection and isolation of Borrelia burgdorferi from rodents. J Clin Microbiol. 1989 Aug;27(8):1723–1727. doi: 10.1128/jcm.27.8.1723-1727.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wilske B., Preac-Mursic V., Schierz G., Kühbeck R., Barbour A. G., Kramer M. Antigenic variability of Borrelia burgdorferi. Ann N Y Acad Sci. 1988;539:126–143. doi: 10.1111/j.1749-6632.1988.tb31846.x. [DOI] [PubMed] [Google Scholar]

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