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. 1996 Jan;34(1):182–184. doi: 10.1128/jcm.34.1.182-184.1996

Comparison of two leukocyte extraction methods for cytomegalovirus antigenemia assay.

A García 1, J Niubò 1, M A Benítez 1, M Viqueira 1, J L Pérez 1
PMCID: PMC228756  PMID: 8748299

Abstract

We carried out a prospective, parallel, and blind study on 113 blood samples from immunocompromised patients in order to compare two leukocyte extraction methods (6% dextran sedimentation and Polymorphprep separation) for cytomegalovirus (CMV) antigenemia assay. CMV was detected in 38 samples by antigenemia assay (34 by dextran sedimentation and 35 by Polymorphprep separation). No differences either in the number of positive specimens (P = 1) or in the mean CMV-positive cell counts (P = 0.41) were observed between the two leukocyte extraction methods. In conclusion, the two methods performed equally well for this assay.

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Selected References

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  1. Dankner W. M., McCutchan J. A., Richman D. D., Hirata K., Spector S. A. Localization of human cytomegalovirus in peripheral blood leukocytes by in situ hybridization. J Infect Dis. 1990 Jan;161(1):31–36. doi: 10.1093/infdis/161.1.31. [DOI] [PubMed] [Google Scholar]
  2. Erice A., Holm M. A., Gill P. C., Henry S., Dirksen C. L., Dunn D. L., Hillam R. P., Balfour H. H., Jr Cytomegalovirus (CMV) antigenemia assay is more sensitive than shell vial cultures for rapid detection of CMV in polymorphonuclear blood leukocytes. J Clin Microbiol. 1992 Nov;30(11):2822–2825. doi: 10.1128/jcm.30.11.2822-2825.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Erice A., Holm M. A., Sanjuan M. V., Dunn D. L., Gill P. C., Balfour H. H., Jr Evaluation of CMV-vue antigenemia assay for rapid detection of cytomegalovirus in mixed-leukocyte blood fractions. J Clin Microbiol. 1995 Apr;33(4):1014–1015. doi: 10.1128/jcm.33.4.1014-1015.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gerna G., Zipeto D., Percivalle E., Parea M., Revello M. G., Maccario R., Peri G., Milanesi G. Human cytomegalovirus infection of the major leukocyte subpopulations and evidence for initial viral replication in polymorphonuclear leukocytes from viremic patients. J Infect Dis. 1992 Dec;166(6):1236–1244. doi: 10.1093/infdis/166.6.1236. [DOI] [PubMed] [Google Scholar]
  5. Gleaves C. A., Smith T. F., Shuster E. A., Pearson G. R. Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens. J Clin Microbiol. 1985 Feb;21(2):217–221. doi: 10.1128/jcm.21.2.217-221.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gregory W. W., Menegus M. A. Practical protocol for cytomegalovirus isolation: use of MRC-5 cell monolayers incubated for 2 weeks. J Clin Microbiol. 1983 Apr;17(4):605–609. doi: 10.1128/jcm.17.4.605-609.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Howell C. L., Miller M. J., Martin W. J. Comparison of rates of virus isolation from leukocyte populations separated from blood by conventional and Ficoll-Paque/Macrodex methods. J Clin Microbiol. 1979 Oct;10(4):533–537. doi: 10.1128/jcm.10.4.533-537.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Leland D. S., Hansing R. L., French M. L. Clinical experience with cytomegalovirus isolation using conventional cell cultures and early antigen detection in centrifugation-enhanced shell vial cultures. J Clin Microbiol. 1989 Jun;27(6):1159–1162. doi: 10.1128/jcm.27.6.1159-1162.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Martínez Lacasa J. T., Podzamczer D., Rufí G., Bolao F., Pérez J. L., Gudiol F. Enfermedad por citomegalovirus en pacientes afectados del síndrome de inmunodeficiencia adquirida. A propósito de 63 episodios. Med Clin (Barc) 1993 Jan 16;100(2):41–45. [PubMed] [Google Scholar]
  10. Meyers J. D., Ljungman P., Fisher L. D. Cytomegalovirus excretion as a predictor of cytomegalovirus disease after marrow transplantation: importance of cytomegalovirus viremia. J Infect Dis. 1990 Aug;162(2):373–380. doi: 10.1093/infdis/162.2.373. [DOI] [PubMed] [Google Scholar]
  11. Pérez J. L., Niubò J., Ardanuy C., Carvajal A., García A., Salvà J., Martínez-Lacasa J. T., Escribà J. M., Martín R. Comparison of three commercially available monoclonal antibodies directed against pp65 antigen for cytomegalovirus antigenemia assay. Diagn Microbiol Infect Dis. 1995 Jan;21(1):21–25. doi: 10.1016/0732-8893(94)00144-l. [DOI] [PubMed] [Google Scholar]
  12. Pérez J. L., Salvà J., Niubó J. La prueba de antigenemia para citomegalovirus. Enferm Infecc Microbiol Clin. 1994 May;12(5):251–261. [PubMed] [Google Scholar]
  13. Salmon D., Lacassin F., Harzic M., Leport C., Perronne C., Bricaire F., Brun-Vezinet F., Vilde J. L. Predictive value of cytomegalovirus viraemia for the occurrence of CMV organ involvement in AIDS. J Med Virol. 1990 Nov;32(3):160–163. doi: 10.1002/jmv.1890320306. [DOI] [PubMed] [Google Scholar]
  14. Taylor-Wiedeman J., Sissons J. G., Borysiewicz L. K., Sinclair J. H. Monocytes are a major site of persistence of human cytomegalovirus in peripheral blood mononuclear cells. J Gen Virol. 1991 Sep;72(Pt 9):2059–2064. doi: 10.1099/0022-1317-72-9-2059. [DOI] [PubMed] [Google Scholar]
  15. The T. H., van der Ploeg M., van den Berg A. P., Vlieger A. M., van der Giessen M., van Son W. J. Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction. Transplantation. 1992 Aug;54(2):193–198. doi: 10.1097/00007890-199208000-00001. [DOI] [PubMed] [Google Scholar]

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