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. 1996 Jan;34(1):199–201. doi: 10.1128/jcm.34.1.199-201.1996

Rapid and sensitive detection of enteroviruses in specimens from patients with aseptic meningitis.

S Yerly 1, A Gervaix 1, V Simonet 1, M Caflisch 1, L Perrin 1, W Wunderli 1
PMCID: PMC228761  PMID: 8748304

Abstract

A 5-h PCR assay (Amplicor enterovirus test) was compared with viral culture for the detection of enteroviruses in cerebrospinal fluid. Of the cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 34% were positive by viral culture whereas 66% were positive by the Amplicor PCR, suggesting that this technique improves the diagnosis of enteroviral meningitis.

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Selected References

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  1. Berlin L. E., Rorabaugh M. L., Heldrich F., Roberts K., Doran T., Modlin J. F. Aseptic meningitis in infants < 2 years of age: diagnosis and etiology. J Infect Dis. 1993 Oct;168(4):888–892. doi: 10.1093/infdis/168.4.888. [DOI] [PubMed] [Google Scholar]
  2. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  3. Chonmaitree T., Menegus M. A., Powell K. R. The clinical relevance of 'CSF viral culture'. A two-year experience with aseptic meningitis in Rochester, NY. JAMA. 1982 Apr 2;247(13):1843–1847. [PubMed] [Google Scholar]
  4. Glimåker M., Johansson B., Olcén P., Ehrnst A., Forsgren M. Detection of enteroviral RNA by polymerase chain reaction in cerebrospinal fluid from patients with aseptic meningitis. Scand J Infect Dis. 1993;25(5):547–557. doi: 10.3109/00365549309008542. [DOI] [PubMed] [Google Scholar]
  5. Hyypiä T., Auvinen P., Maaronen M. Polymerase chain reaction for human picornaviruses. J Gen Virol. 1989 Dec;70(Pt 12):3261–3268. doi: 10.1099/0022-1317-70-12-3261. [DOI] [PubMed] [Google Scholar]
  6. Melnick J. L., Wimberly I. L. Lyophilized combination pools of enterovirus equine antisera: new LBM pools prepared from reserves of antisera stored frozen for two decades. Bull World Health Organ. 1985;63(3):543–550. [PMC free article] [PubMed] [Google Scholar]
  7. Muir P., Nicholson F., Jhetam M., Neogi S., Banatvala J. E. Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens. J Clin Microbiol. 1993 Jan;31(1):31–38. doi: 10.1128/jcm.31.1.31-38.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Petitjean J., Freymuth F., Kopecka H., Brouard J., Boutard P., Lebon P. Detection of enteroviruses in cerebrospinal fluids: enzymatic amplification and hybridization with a biotinylated riboprobe. Mol Cell Probes. 1994 Feb;8(1):15–22. doi: 10.1006/mcpr.1994.1003. [DOI] [PubMed] [Google Scholar]
  9. Rotbart H. A. Diagnosis of enteroviral meningitis with the polymerase chain reaction. J Pediatr. 1990 Jul;117(1 Pt 1):85–89. doi: 10.1016/s0022-3476(05)82451-5. [DOI] [PubMed] [Google Scholar]
  10. Rotbart H. A., Sawyer M. H., Fast S., Lewinski C., Murphy N., Keyser E. F., Spadoro J., Kao S. Y., Loeffelholz M. Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay. J Clin Microbiol. 1994 Oct;32(10):2590–2592. doi: 10.1128/jcm.32.10.2590-2592.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sawyer M. H., Holland D., Aintablian N., Connor J. D., Keyser E. F., Waecker N. J., Jr Diagnosis of enteroviral central nervous system infection by polymerase chain reaction during a large community outbreak. Pediatr Infect Dis J. 1994 Mar;13(3):177–182. doi: 10.1097/00006454-199403000-00002. [DOI] [PubMed] [Google Scholar]
  12. Schlesinger Y., Sawyer M. H., Storch G. A. Enteroviral meningitis in infancy: potential role for polymerase chain reaction in patient management. Pediatrics. 1994 Aug;94(2 Pt 1):157–162. [PubMed] [Google Scholar]
  13. Schmidt N. J., Ho H. H., Lennette E. H. Propagation and isolation of group A coxsackieviruses in RD cells. J Clin Microbiol. 1975 Sep;2(3):183–185. doi: 10.1128/jcm.2.3.183-185.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Singer J. I., Maur P. R., Riley J. P., Smith P. B. Management of central nervous system infections during an epidemic of enteroviral aseptic meningitis. J Pediatr. 1980 Mar;96(3 Pt 2):559–563. doi: 10.1016/s0022-3476(80)80866-3. [DOI] [PubMed] [Google Scholar]
  15. Sköldenberg B. On the role of viruses in acute infectious diseases of the central nervous system. Clinical and laboratory studies on hospitalized patients. Scand J Infect Dis. 1972;3(Suppl):1–96. doi: 10.3109/inf.1972.4.suppl-3.01. [DOI] [PubMed] [Google Scholar]
  16. Sumaya C. V., Corman L. I. Enteroviral meningitis in early infancy: significance in community outbreaks. Pediatr Infect Dis. 1982 May-Jun;1(3):151–154. doi: 10.1097/00006454-198205000-00004. [DOI] [PubMed] [Google Scholar]
  17. Wilfert C. M., Lehrman S. N., Katz S. L. Enteroviruses and meningitis. Pediatr Infect Dis. 1983 Jul-Aug;2(4):333–341. doi: 10.1097/00006454-198307000-00019. [DOI] [PubMed] [Google Scholar]

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