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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1996 Feb;34(2):457–459. doi: 10.1128/jcm.34.2.457-459.1996

High-level sensitivity of quantitative pp65 cytomegalovirus (CMV) antigenemia assay for diagnosis of CMV disease in AIDS patients and follow-up.

B Bek 1, M Boeckh 1, J Lepenies 1, B Bieniek 1, K Arasteh 1, W Heise 1, K M Deppermann 1, G Bornhöft 1, M Stöffler-Meilicke 1, I Schuller 1, G Höffken 1
PMCID: PMC228821  PMID: 8789039

Abstract

Cytomegalovirus (CMV) antigenemia was evaluated in 174 patients positive for human immunodeficiency virus. Antigenemia could be detected in 96.7% of patients with CMV disease, 76.9% of patients suffering from a relapse of the disease, and 11.4% of asymptomatic patients with CD4 levels of < 100 cells per microliter. No antigenemia was detected in patients with CD4 levels of 250 to 500 cells per microliter. Specificity and the positive predictive value for CMV disease were increased only if more than 5 positive cells per slide were considered. However, CMV disease may also occur in patients with low-grade antigenemia.

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Selected References

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  1. Boeckh M., Bowden R. A., Goodrich J. M., Pettinger M., Meyers J. D. Cytomegalovirus antigen detection in peripheral blood leukocytes after allogeneic marrow transplantation. Blood. 1992 Sep 1;80(5):1358–1364. [PubMed] [Google Scholar]
  2. Francisci D., Tosti A., Preziosi R., Baldelli F., Stagni G., Pauluzzi S. Role of antigenemia assay in the early diagnosis and prediction of human cytomegalovirus organ involvement in AIDS patients. Eur J Clin Microbiol Infect Dis. 1995 Jun;14(6):498–503. doi: 10.1007/BF02113427. [DOI] [PubMed] [Google Scholar]
  3. Gerna G., Parea M., Percivalle E., Zipeto D., Silini E., Barbarini G., Milanesi G. Human cytomegalovirus viraemia in HIV-1-seropositive patients at various clinical stages of infection. AIDS. 1990 Oct;4(10):1027–1031. doi: 10.1097/00002030-199010000-00014. [DOI] [PubMed] [Google Scholar]
  4. Gerna G., Revello M. G., Percivalle E., Zavattoni M., Parea M., Battaglia M. Quantification of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins. J Clin Microbiol. 1990 Dec;28(12):2681–2688. doi: 10.1128/jcm.28.12.2681-2688.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerna G., Zipeto D., Parea M., Percivalle E., Zavattoni M., Gaballo A., Milanesi G. Early virus isolation, early structural antigen detection and DNA amplification by the polymerase chain reaction in polymorphonuclear leukocytes from AIDS patients with human cytomegalovirus viraemia. Mol Cell Probes. 1991 Oct;5(5):365–374. doi: 10.1016/s0890-8508(06)80008-3. [DOI] [PubMed] [Google Scholar]
  6. Guarda L. A., Luna M. A., Smith J. L., Jr, Mansell P. W., Gyorkey F., Roca A. N. Acquired immune deficiency syndrome: postmortem findings. Am J Clin Pathol. 1984 May;81(5):549–557. doi: 10.1093/ajcp/81.5.549. [DOI] [PubMed] [Google Scholar]
  7. Landry M. L., Ferguson D. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J Clin Microbiol. 1993 Nov;31(11):2851–2856. doi: 10.1128/jcm.31.11.2851-2856.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lipson S. M., Kaplan M. H., Tseng L. F., Mandel F. S. Use of the cytomegalovirus antigenemia (CMV-Ag) assay for the detection of CMV in the blood of AIDS patients. Can J Microbiol. 1993 Nov;39(11):1059–1065. doi: 10.1139/m93-160. [DOI] [PubMed] [Google Scholar]
  9. Mastroianni C. M., Sebastiani G., Folgori F., Ajassa C., Vullo V., Volpi A. Detection of cytomegalovirus-matrix protein (pp65) in leukocytes of HIV-infected patients with painful peripheral neuropathy. J Med Virol. 1994 Oct;44(2):172–175. doi: 10.1002/jmv.1890440210. [DOI] [PubMed] [Google Scholar]
  10. Mazzulli T., Rubin R. H., Ferraro M. J., D'Aquila R. T., Doveikis S. A., Smith B. R., The T. H., Hirsch M. S. Cytomegalovirus antigenemia: clinical correlations in transplant recipients and in persons with AIDS. J Clin Microbiol. 1993 Oct;31(10):2824–2827. doi: 10.1128/jcm.31.10.2824-2827.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reichert C. M., O'Leary T. J., Levens D. L., Simrell C. R., Macher A. M. Autopsy pathology in the acquired immune deficiency syndrome. Am J Pathol. 1983 Sep;112(3):357–382. [PMC free article] [PubMed] [Google Scholar]
  12. d'Arminio Monforte A., Vago L., Lazzarin A., Boldorini R., Bini T., Guzzetti S., Antinori S., Moroni M., Costanzi G. AIDS-defining diseases in 250 HIV-infected patients; a comparative study of clinical and autopsy diagnoses. AIDS. 1992 Oct;6(10):1159–1164. doi: 10.1097/00002030-199210000-00015. [DOI] [PubMed] [Google Scholar]

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