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. 1996 Feb;34(2):466–470. doi: 10.1128/jcm.34.2.466-470.1996

Serotyping of Cryptococcus neoformans by dot enzyme assay.

T Belay 1, R Cherniak 1, E B O'Neill 1, T R Kozel 1
PMCID: PMC228824  PMID: 8789042

Abstract

A method is described for the serotyping of Cryptococcus neoformans based on direct analysis of culture supernatants for the major type-specific capsular antigen, glucuronoxylomannan. Factor sera prepared by absorption of polyclonal rabbit antisera (Iatron Laboratories, Inc., Tokyo, Japan) or selected anti-C. neoformans monoclonal antibodies were used in a dot enzyme assay to detect the presence of antigen.

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Selected References

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