Abstract
Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per microliter. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per microliter.
Full Text
The Full Text of this article is available as a PDF (181.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gozlan J., Salord J. M., Roullet E., Baudrimont M., Caburet F., Picard O., Meyohas M. C., Duvivier C., Jacomet C., Petit J. C. Rapid detection of cytomegalovirus DNA in cerebrospinal fluid of AIDS patients with neurologic disorders. J Infect Dis. 1992 Dec;166(6):1416–1421. doi: 10.1093/infdis/166.6.1416. [DOI] [PubMed] [Google Scholar]
- Ho D. D., Rota T. R., Schooley R. T., Kaplan J. C., Allan J. D., Groopman J. E., Resnick L., Felsenstein D., Andrews C. A., Hirsch M. S. Isolation of HTLV-III from cerebrospinal fluid and neural tissues of patients with neurologic syndromes related to the acquired immunodeficiency syndrome. N Engl J Med. 1985 Dec 12;313(24):1493–1497. doi: 10.1056/NEJM198512123132401. [DOI] [PubMed] [Google Scholar]
- Navia B. A., Cho E. S., Petito C. K., Price R. W. The AIDS dementia complex: II. Neuropathology. Ann Neurol. 1986 Jun;19(6):525–535. doi: 10.1002/ana.410190603. [DOI] [PubMed] [Google Scholar]
- Sakrauski A., Weber B., Kessler H. H., Pierer K., Doerr H. W. Comparison of two hybridization assays for the rapid detection of PCR amplified HSV genome sequences from cerebrospinal fluid. J Virol Methods. 1994 Dec;50(1-3):175–184. doi: 10.1016/0166-0934(94)90174-0. [DOI] [PubMed] [Google Scholar]
- Schacherer C., Braun W., Bauer G., Doerr H. W. Detection of cytomegalovirus in bronchial lavage and urine using a monoclonal antibody to an HCMV early nuclear protein. Infection. 1988 Sep-Oct;16(5):288–292. doi: 10.1007/BF01645074. [DOI] [PubMed] [Google Scholar]
- Troendle Atkins J., Demmler G. J., Williamson W. D., McDonald J. M., Istas A. S., Buffone G. J. Polymerase chain reaction to detect cytomegalovirus DNA in the cerebrospinal fluid of neonates with congenital infection. J Infect Dis. 1994 Jun;169(6):1334–1337. doi: 10.1093/infdis/169.6.1334. [DOI] [PubMed] [Google Scholar]
- Walsh P. S., Metzger D. A., Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques. 1991 Apr;10(4):506–513. [PubMed] [Google Scholar]
- Weber B., Nestler U., Ernst W., Rabenau H., Braner J., Birkenbach A., Scheuermann E. H., Schoeppe W., Doerr H. W. Low correlation of human cytomegalovirus DNA amplification by polymerase chain reaction with cytomegalovirus disease in organ transplant recipients. J Med Virol. 1994 Jun;43(2):187–193. doi: 10.1002/jmv.1890430217. [DOI] [PubMed] [Google Scholar]