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. 1996 Mar;34(3):588–591. doi: 10.1128/jcm.34.3.588-591.1996

Comparison of broth microdilution and E-test for susceptibility testing of Neisseria meningitidis.

A Pascual 1, P Joyanes 1, L Martinez-Martinez 1, A I Suarez 1, E J Perea 1
PMCID: PMC228852  PMID: 8904420

Abstract

The susceptibilities of 54 clinical isolates of Neisseria meningitidis to penicillin, cefotaxime, ceftriaxone, cefepime, imipenem, ciprofloxacin, chloramphenicol, and rifampin were determined by the microdilution method in both cation-adjusted Mueller-Hinton broth (CAMHB) and Haemophilus test medium (HTM). Poor growth was observed in 16.6 and 9% of the strains in CAMHB and HTM, respectively. As a result, the growth of the 54 N. meningitidis strains was evaluated in three other commercially available batches of CAMHB and in one in-house batch of HTM. Poor growth was observed for 9.3 to 16.6% of the strains in all four batches. More important, three of the CAMHB batches failed to support growth for 3.7 to 33.3% of the strains; 3.7% of the strains did not grow in the in-house-prepared HTM. Ten (18.7%) strains were relatively resistant to penicillin (RRP; MIC, > 0.125 mu g/ml) in CAMHB and 13 (24%) strains were RRP in HTM. The percentages of agreement obtained by using CAMHB as the reference ranged from 78% for cefepime to 100% for ceftriaxone. Seven minor errors were observed for penicillin; five of them were for strains susceptible to penicillin in CAMHB and RRP in HTM. All strains were susceptible to the other antimicrobial agents evaluated. The growth of N. meningitidis was also evaluated in four batches of Mueller-Hinton agar (MHA). In two of them, 3.7 and 44.4% of the strains did not grow, and considering all four batches, 5.5 to 11.1% grew poorly. All strains grew adequately in MHA supplemented with blood (MHA-b). The activities of penicillin and cefotaxime were also evaluated by the E-test in MHA and MHA-b. The proportion of RRP strains were 24% in MHA and 59% in MHA-b. For penicillin, the percentages of agreement of the E-test with the microdilution method in CAMHB (reference) were 64.8 and 70.3% in MHA and MHA-b, respectively. For cefotaxime, the agreement was 98.1%. Minor errors for the penicillin MIC were detected for 38% of the strains tested. Further studies are needed to define adequate culture media for reference methods to evaluate the susceptibility of N. meningitidis to antimicrobial agents.

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Selected References

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  1. Berrón S., Vázquez J. A. Increase in moderate penicillin resistance and serogroup C in meningococcal strains isolated in Spain. Is there any relationship? Clin Infect Dis. 1994 Feb;18(2):161–165. doi: 10.1093/clinids/18.2.161. [DOI] [PubMed] [Google Scholar]
  2. Botha P. Penicillin-resistant Neisseria meningitidis in southern Africa. Lancet. 1988 Jan 2;1(8575-6):54–54. doi: 10.1016/s0140-6736(88)91029-x. [DOI] [PubMed] [Google Scholar]
  3. Campos J., Mendelman P. M., Sako M. U., Chaffin D. O., Smith A. L., Sáez-Nieto J. A. Detection of relatively penicillin G-resistant Neisseria meningitidis by disk susceptibility testing. Antimicrob Agents Chemother. 1987 Oct;31(10):1478–1482. doi: 10.1128/aac.31.10.1478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campos J., Trujillo G., Seuba T., Rodriguez A. Discriminative criteria for Neisseria meningitidis isolates that are moderately susceptible to penicillin and ampicillin. Antimicrob Agents Chemother. 1992 May;36(5):1028–1031. doi: 10.1128/aac.36.5.1028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dillon J. R., Pauzé M., Yeung K. H. Spread of penicillinase-producing and transfer plasmids from the gonococcus to Neisseria meningitidis. Lancet. 1983 Apr 9;1(8328):779–781. doi: 10.1016/s0140-6736(83)91846-9. [DOI] [PubMed] [Google Scholar]
  6. Fontanals D., Pineda V., Pons I., Rojo J. C. Penicillin-resistant beta-lactamase producing Neisseria meningitidis in Spain. Eur J Clin Microbiol Infect Dis. 1989 Jan;8(1):90–91. doi: 10.1007/BF01964130. [DOI] [PubMed] [Google Scholar]
  7. Hughes J. H., Biedenbach D. J., Erwin M. E., Jones R. N. E test as susceptibility test and epidemiologic tool for evaluation of Neisseria meningitidis isolates. J Clin Microbiol. 1993 Dec;31(12):3255–3259. doi: 10.1128/jcm.31.12.3255-3259.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jackson L. A., Tenover F. C., Baker C., Plikaytis B. D., Reeves M. W., Stocker S. A., Weaver R. E., Wenger J. D. Prevalence of Neisseria meningitidis relatively resistant to penicillin in the United States, 1991. Meningococcal Disease Study Group. J Infect Dis. 1994 Feb;169(2):438–441. doi: 10.1093/infdis/169.2.438. [DOI] [PubMed] [Google Scholar]
  9. Lopardo H. A., Santander C., Ceinos M. C., Rubeglio E. A. Isolation of moderately penicillin-susceptible strains of Neisseria meningitidis in Argentina. Antimicrob Agents Chemother. 1993 Aug;37(8):1728–1729. doi: 10.1128/aac.37.8.1728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mendelman P. M., Campos J., Chaffin D. O., Serfass D. A., Smith A. L., Sáez-Nieto J. A. Relative penicillin G resistance in Neisseria meningitidis and reduced affinity of penicillin-binding protein 3. Antimicrob Agents Chemother. 1988 May;32(5):706–709. doi: 10.1128/aac.32.5.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Miller L. A., Rittenhouse S. F., Utrup L. J., Poupard J. A. Comparison of three methods for determination of a single MIC of an antimicrobial agent. J Clin Microbiol. 1994 May;32(5):1373–1375. doi: 10.1128/jcm.32.5.1373-1375.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Penicillin-resistant strains of Neisseria meningitidis in Spain. Lancet. 1988 Jun 25;1(8600):1452–1453. [PubMed] [Google Scholar]
  13. Pinner R. W., Gellin B. G., Bibb W. F., Baker C. N., Weaver R., Hunter S. B., Waterman S. H., Mocca L. F., Frasch C. E., Broome C. V. Meningococcal disease in the United States--1986. Meningococcal Disease Study Group. J Infect Dis. 1991 Aug;164(2):368–374. doi: 10.1093/infdis/164.2.368. [DOI] [PubMed] [Google Scholar]
  14. Pérez Trallero E., Garcia Arenzana J. M., Ayestaran I., Muñoz Baroja I. Comparative activity in vitro of 16 antimicrobial agents against penicillin-susceptible meningococci and meningococci with diminished susceptibility to penicillin. Antimicrob Agents Chemother. 1989 Sep;33(9):1622–1623. doi: 10.1128/aac.33.9.1622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pérez-Trallero E., Gomez N., García-Arenzana J. M. E test as susceptibility test for evaluation of Neisseria meningitidis isolates. J Clin Microbiol. 1994 Sep;32(9):2341–2342. doi: 10.1128/jcm.32.9.2341-2342.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ringuette L., Lorange M., Ryan A., Ashton F. Meningococcal infections in the Province of Québec, Canada, during the period 1991 to 1992. J Clin Microbiol. 1995 Jan;33(1):53–57. doi: 10.1128/jcm.33.1.53-57.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sutcliffe E. M., Jones D. M., el-Sheikh S., Percival A. Penicillin-insensitive meningococci in the UK. Lancet. 1988 Mar 19;1(8586):657–658. doi: 10.1016/s0140-6736(88)91469-9. [DOI] [PubMed] [Google Scholar]
  18. Sáez-Nieto J. A., Fontanals D., Garcia de Jalon J., Martinez de Artola V., Peña P., Morera M. A., Verdaguer R., Sanfeliu I., Belio-Blasco C., Perez-Saenz J. L. Isolation of Neisseria meningitidis strains with increase of penicillin minimal inhibitory concentrations. Epidemiol Infect. 1987 Oct;99(2):463–469. doi: 10.1017/s0950268800067960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Winterscheid K. K., Whittington W. L., Roberts M. C., Schwebke J. R., Holmes K. K. Decreased susceptibility to penicillin G and Tet M plasmids in genital and anorectal isolates of Neisseria meningitidis. Antimicrob Agents Chemother. 1994 Jul;38(7):1661–1663. doi: 10.1128/aac.38.7.1661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woods C. R., Smith A. L., Wasilauskas B. L., Campos J., Givner L. B. Invasive disease caused by Neisseria meningitidis relatively resistant to penicillin in North Carolina. J Infect Dis. 1994 Aug;170(2):453–456. doi: 10.1093/infdis/170.2.453. [DOI] [PubMed] [Google Scholar]

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