Abstract
Bordetella parapertussis, previously thought to be an obligate human respiratory tract pathogen, has been isolated from sheep. Attempts to assess the prevalence of B. parapertussis in conventionally reared sheep by nasal swabbing proved futile with existing selective media because of extensive overgrowth with Mucor spp. and other nasal commensals. Moredun Bordetella Medium (MBM), which contains cycloheximide and spectinomycin at final concentrations of 0.5 mg/ml and 100 mu g/ml, respectively, was developed as an improved selective medium to isolate B. parapertussis from the nasal cavities of conventionally reared sheep. The selective ability of MBM was evaluated with 200 nasal swabs from conventionally reared sheep, and B. parapertussis was recovered from 31.5% of the samples. MBM facilitated the simple and effective isolation of B. parapertussis from ovine nasal swabs and, in successfully excluding overgrowth with other contaminants, proved superior to other test formulations evaluated and to existing conventional media.
Full Text
The Full Text of this article is available as a PDF (272.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alley M. R. The bacterial flora of the respiratory tract of normal and pneumonic sheep. N Z Vet J. 1975 Jun;23(6):113–118. doi: 10.1080/00480169.1975.34211. [DOI] [PubMed] [Google Scholar]
- Cullinane L. C., Alley M. R., Marshall R. B., Manktelow B. W. Bordetella parapertussis from lambs. N Z Vet J. 1987 Oct;35(10):175–175. doi: 10.1080/00480169.1987.35433. [DOI] [PubMed] [Google Scholar]
- Eldering G., Kendrick P. BACILLUS PARA-PERTUSSIS: A SPECIES RESEMBLING BOTH BACILLUS PERTUSSIS AND BACILLUS BRONCHISEPTICUS BUT IDENTICAL WITH NEITHER. J Bacteriol. 1938 Jun;35(6):561–572. doi: 10.1128/jb.35.6.561-572.1938. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kurzynski T. A., Boehm D. M., Rott-Petri J. A., Schell R. F., Allison P. E. Comparison of modified Bordet-Gengou and modified Regan-Lowe media for the isolation of Bordetella pertussis and Bordetella parapertussis. J Clin Microbiol. 1988 Dec;26(12):2661–2663. doi: 10.1128/jcm.26.12.2661-2663.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Linnemann C. C., Perry E. B. Bordetella parapertussis. Recent experience and a review of the literature. Am J Dis Child. 1977 May;131(5):560–563. doi: 10.1001/archpedi.1977.02120180074014. [DOI] [PubMed] [Google Scholar]
- Magwood S. E., Barnum D. A., Thomson R. G. Nasal bacterial flora of calves in healthy and in pneumonia-prone herds. Can J Comp Med. 1969 Oct;33(4):237–243. [PMC free article] [PubMed] [Google Scholar]
- Porter J. F., Connor K., Donachie W. Isolation and characterization of Bordetella parapertussis-like bacteria from ovine lungs. Microbiology. 1994 Feb;140(Pt 2):255–261. doi: 10.1099/13500872-140-2-255. [DOI] [PubMed] [Google Scholar]
- Regan J., Lowe F. Enrichment medium for the isolation of Bordetella. J Clin Microbiol. 1977 Sep;6(3):303–309. doi: 10.1128/jcm.6.3.303-309.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith I. M., Baskerville A. J. A selective medium facilitating the isolation and recognition of Bordetella bronchiseptica in pigs. Res Vet Sci. 1979 Sep;27(2):187–192. [PubMed] [Google Scholar]
- Sutcliffe E. M., Abbott J. D. Selective medium for the isolation of bordetella pertussis and parapertussis. J Clin Pathol. 1972 Aug;25(8):732–733. doi: 10.1136/jcp.25.8.732. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vandamme P., Hommez J., Vancanneyt M., Monsieurs M., Hoste B., Cookson B., Wirsing von König C. H., Kersters K., Blackall P. J. Bordetella hinzii sp. nov., isolated from poultry and humans. Int J Syst Bacteriol. 1995 Jan;45(1):37–45. doi: 10.1099/00207713-45-1-37. [DOI] [PubMed] [Google Scholar]
- Weyant R. S., Hollis D. G., Weaver R. E., Amin M. F., Steigerwalt A. G., O'Connor S. P., Whitney A. M., Daneshvar M. I., Moss C. W., Brenner D. J. Bordetella holmesii sp. nov., a new gram-negative species associated with septicemia. J Clin Microbiol. 1995 Jan;33(1):1–7. doi: 10.1128/jcm.33.1.1-7.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]