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. 1996 Mar;34(3):720–723. doi: 10.1128/jcm.34.3.720-723.1996

Evaluation of ribotyping as epidemiologic tool for typing Escherichia coli serogroup O157 isolates.

I E Martin 1, S D Tyler 1, K D Tyler 1, R Khakhria 1, W M Johnson 1
PMCID: PMC228877  PMID: 8904445

Abstract

A total of 121 representative Escherichia coli O157:H7 and O157:NM (nonmotile) isolates were characterized by ribotype, phage type, verotoxin genotype, and genomic fingerprints generated by pulsed-field gel electrophoresis. Ribotyping was not able to discriminate between O157:H7 isolates, and phage typing and pulsed-field gel electrophoresis were the most valuable and discriminatory techniques.

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Selected References

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  1. Ahmed R., Bopp C., Borczyk A., Kasatiya S. Phage-typing scheme for Escherichia coli O157:H7. J Infect Dis. 1987 Apr;155(4):806–809. doi: 10.1093/infdis/155.4.806. [DOI] [PubMed] [Google Scholar]
  2. Barrett T. J., Lior H., Green J. H., Khakhria R., Wells J. G., Bell B. P., Greene K. D., Lewis J., Griffin P. M. Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel electrophoresis and phage typing. J Clin Microbiol. 1994 Dec;32(12):3013–3017. doi: 10.1128/jcm.32.12.3013-3017.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brosius J., Ullrich A., Raker M. A., Gray A., Dull T. J., Gutell R. R., Noller H. F. Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid. 1981 Jul;6(1):112–118. doi: 10.1016/0147-619x(81)90058-5. [DOI] [PubMed] [Google Scholar]
  4. Böhm H., Karch H. DNA fingerprinting of Escherichia coli O157:H7 strains by pulsed-field gel electrophoresis. J Clin Microbiol. 1992 Aug;30(8):2169–2172. doi: 10.1128/jcm.30.8.2169-2172.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Karmali M. A. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev. 1989 Jan;2(1):15–38. doi: 10.1128/cmr.2.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Khakhria R., Duck D., Lior H. Extended phage-typing scheme for Escherichia coli O157:H7. Epidemiol Infect. 1990 Dec;105(3):511–520. doi: 10.1017/s0950268800048135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Martinetti G., Altwegg M. rRNA gene restriction patterns and plasmid analysis as a tool for typing Salmonella enteritidis. Res Microbiol. 1990 Nov-Dec;141(9):1151–1162. doi: 10.1016/0923-2508(90)90088-8. [DOI] [PubMed] [Google Scholar]
  8. Ostroff S. M., Tarr P. I., Neill M. A., Lewis J. H., Hargrett-Bean N., Kobayashi J. M. Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections. J Infect Dis. 1989 Dec;160(6):994–998. doi: 10.1093/infdis/160.6.994. [DOI] [PubMed] [Google Scholar]
  9. Pollard D. R., Johnson W. M., Lior H., Tyler S. D., Rozee K. R. Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction. J Clin Microbiol. 1990 Mar;28(3):540–545. doi: 10.1128/jcm.28.3.540-545.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Samadpour M., Grimm L. M., Desai B., Alfi D., Ongerth J. E., Tarr P. I. Molecular epidemiology of Escherichia coli O157:H7 strains by bacteriophage lambda restriction fragment length polymorphism analysis: application to a multistate foodborne outbreak and a day-care center cluster. J Clin Microbiol. 1993 Dec;31(12):3179–3183. doi: 10.1128/jcm.31.12.3179-3183.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Samadpour M. Molecular epidemiology of Escherichia coli O157:H7 by restriction fragment length polymorphism using Shiga-like toxin genes. J Clin Microbiol. 1995 Aug;33(8):2150–2154. doi: 10.1128/jcm.33.8.2150-2154.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stull T. L., LiPuma J. J., Edlind T. D. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis. 1988 Feb;157(2):280–286. doi: 10.1093/infdis/157.2.280. [DOI] [PubMed] [Google Scholar]
  13. Tarkka E., Ahman H., Siitonen A. Ribotyping as an epidemiologic tool for Escherichia coli. Epidemiol Infect. 1994 Apr;112(2):263–274. doi: 10.1017/s0950268800057678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tyler S. D., Johnson W. M., Lior H., Wang G., Rozee K. R. Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysis. J Clin Microbiol. 1991 Jul;29(7):1339–1343. doi: 10.1128/jcm.29.7.1339-1343.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wachsmuth I. K., Kiehlbauch J. A., Bopp C. A., Cameron D. N., Strockbine N. A., Wells J. G., Blake P. A. The use of plasmid profiles and nucleic acid probes in epidemiologic investigations of foodborne, diarrheal diseases. Int J Food Microbiol. 1991 Jan;12(1):77–89. doi: 10.1016/0168-1605(91)90049-u. [DOI] [PubMed] [Google Scholar]
  16. Wells J. G., Davis B. R., Wachsmuth I. K., Riley L. W., Remis R. S., Sokolow R., Morris G. K. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J Clin Microbiol. 1983 Sep;18(3):512–520. doi: 10.1128/jcm.18.3.512-520.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Whittam T. S., Wachsmuth I. K., Wilson R. A. Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis. 1988 Jun;157(6):1124–1133. doi: 10.1093/infdis/157.6.1124. [DOI] [PubMed] [Google Scholar]
  18. Whittam T. S., Wolfe M. L., Wachsmuth I. K., Orskov F., Orskov I., Wilson R. A. Clonal relationships among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea. Infect Immun. 1993 May;61(5):1619–1629. doi: 10.1128/iai.61.5.1619-1629.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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