Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1996 Mar;34(3):745–747. doi: 10.1128/jcm.34.3.745-747.1996

Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas.

M F Baay 1, W G Quint 1, J Koudstaal 1, H Hollema 1, J M Duk 1, M P Burger 1, E Stolz 1, P Herbrink 1
PMCID: PMC228883  PMID: 8904451

Abstract

We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (GP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.

Full Text

The Full Text of this article is available as a PDF (145.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Ezra J., Johnson D. A., Rossi J., Cook N., Wu A. Effect of fixation on the amplification of nucleic acids from paraffin-embedded material by the polymerase chain reaction. J Histochem Cytochem. 1991 Mar;39(3):351–354. doi: 10.1177/39.3.1704393. [DOI] [PubMed] [Google Scholar]
  2. Bianchi A. B., Navone N. M., Conti C. J. Detection of loss of heterozygosity in formalin-fixed paraffin-embedded tumor specimens by the polymerase chain reaction. Am J Pathol. 1991 Feb;138(2):279–284. [PMC free article] [PubMed] [Google Scholar]
  3. Burnett A. F., Barnes W. A., Johnson J. C., Grendys E., Willett G. D., Barter J. F., Doniger J. Prognostic significance of polymerase chain reaction detected human papillomavirus of tumors and lymph nodes in surgically treated stage IB cervical cancer. Gynecol Oncol. 1992 Dec;47(3):343–347. doi: 10.1016/0090-8258(92)90137-8. [DOI] [PubMed] [Google Scholar]
  4. Falcinelli C., Claas E., Kleter B., Quint W. G. Detection of the human papilloma virus type 16 mRNA-transcripts in cytological abnormal scrapings. J Med Virol. 1992 Jun;37(2):93–98. doi: 10.1002/jmv.1890370204. [DOI] [PubMed] [Google Scholar]
  5. Goodrow T. L., Prahalada S. R., Storer R. D., Manam S. V., Leander K. R., Kraynak A. R., van Zwieten M. J., Nichols W. W., Bradley M. O. Polymerase chain reaction/sequencing analysis of ras mutations in paraffin-embedded tissues as compared with 3T3 transfection and polymerase chain reaction/sequencing of frozen tumor deoxyribonucleic acids. Lab Invest. 1992 Apr;66(4):504–511. [PubMed] [Google Scholar]
  6. Gregoire L., Arella M., Campione-Piccardo J., Lancaster W. D. Amplification of human papillomavirus DNA sequences by using conserved primers. J Clin Microbiol. 1989 Dec;27(12):2660–2665. doi: 10.1128/jcm.27.12.2660-2665.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karlsen F., Kalantari M., Chitemerere M., Johansson B., Hagmar B. Modifications of human and viral deoxyribonucleic acid by formaldehyde fixation. Lab Invest. 1994 Oct;71(4):604–611. [PubMed] [Google Scholar]
  8. Karlsen F., Kristensen G., Holm R., Chitemerere M., Berner A., Hagmar B. M. High incidence of human papillomavirus in 146 cervical carcinomas. A study using three different pairs of consensus primers, and detecting viral genomes with putative deletions. Eur J Cancer. 1995;31A(9):1511–1516. doi: 10.1016/0959-8049(95)00206-x. [DOI] [PubMed] [Google Scholar]
  9. Melchers W., van den Brule A., Walboomers J., de Bruin M., Burger M., Herbrink P., Meijer C., Lindeman J., Quint W. Increased detection rate of human papillomavirus in cervical scrapes by the polymerase chain reaction as compared to modified FISH and southern-blot analysis. J Med Virol. 1989 Apr;27(4):329–335. doi: 10.1002/jmv.1890270413. [DOI] [PubMed] [Google Scholar]
  10. Muñoz N., Bosch F. X., de Sanjosé S., Tafur L., Izarzugaza I., Gili M., Viladiu P., Navarro C., Martos C., Ascunce N. The causal link between human papillomavirus and invasive cervical cancer: a population-based case-control study in Colombia and Spain. Int J Cancer. 1992 Nov 11;52(5):743–749. doi: 10.1002/ijc.2910520513. [DOI] [PubMed] [Google Scholar]
  11. Resnick R. M., Cornelissen M. T., Wright D. K., Eichinger G. H., Fox H. S., ter Schegget J., Manos M. M. Detection and typing of human papillomavirus in archival cervical cancer specimens by DNA amplification with consensus primers. J Natl Cancer Inst. 1990 Sep 19;82(18):1477–1484. doi: 10.1093/jnci/82.18.1477. [DOI] [PubMed] [Google Scholar]
  12. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  13. Snijders P. J., van den Brule A. J., Schrijnemakers H. F., Snow G., Meijer C. J., Walboomers J. M. The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes. J Gen Virol. 1990 Jan;71(Pt 1):173–181. doi: 10.1099/0022-1317-71-1-173. [DOI] [PubMed] [Google Scholar]
  14. Thompson C. H., Rose B. R. Deleterious effects of formalin/acetic acid/alcohol (FAA) fixation on the detection of HPV DNA by in situ hybridization and the polymerase chain reaction. Pathology. 1991 Oct;23(4):327–330. doi: 10.3109/00313029109063598. [DOI] [PubMed] [Google Scholar]
  15. Tieben L. M., ter Schegget J., Minnaar R. P., Bouwes Bavinck J. N., Berkhout R. J., Vermeer B. J., Jebbink M. F., Smits H. L. Detection of cutaneous and genital HPV types in clinical samples by PCR using consensus primers. J Virol Methods. 1993 May;42(2-3):265–279. doi: 10.1016/0166-0934(93)90038-s. [DOI] [PubMed] [Google Scholar]
  16. Yoshikawa H., Kawana T., Kitagawa K., Mizuno M., Yoshikura H., Iwamoto A. Detection and typing of multiple genital human papillomaviruses by DNA amplification with consensus primers. Jpn J Cancer Res. 1991 May;82(5):524–531. doi: 10.1111/j.1349-7006.1991.tb01882.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. van den Brule A. J., Snijders P. J., Gordijn R. L., Bleker O. P., Meijer C. J., Walboomers J. M. General primer-mediated polymerase chain reaction permits the detection of sequenced and still unsequenced human papillomavirus genotypes in cervical scrapes and carcinomas. Int J Cancer. 1990 Apr 15;45(4):644–649. doi: 10.1002/ijc.2910450412. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES