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. 1991 Apr 1;113(1):147–154. doi: 10.1083/jcb.113.1.147

Heterogeneity of microvascular pericytes for smooth muscle type alpha- actin

PMCID: PMC2288926  PMID: 2007619

Abstract

Microvascular pericytes are believed to be involved in various functions such as regulation of capillary blood flow and endothelial proliferation. Since pericytes represent a morphologically heterogeneous cell population ranging from circular smooth musclelike to elongated fibroblast-like morphology it is possible that regulation of blood flow (via contractility) and control of endothelial proliferation (as well as other metabolic functions) may be accomplished by different subsets of pericytes. In the present study we provide evidence for heterogeneity of pericytes at the molecular level by using two novel technical approaches. These are (a) immunostaining of whole mounts of the microvascular beds of the rat mesentery and bovine retina and (b) immunoblotting studies of microdissected retinal microvessels. We show that pericytes of true capillaries (midcapillaries) apparently lack the smooth muscle isoform of alpha- actin whereas transitional pericytes of pre- and postcapillary microvascular segments do express this isoform. Thus, regulation of capillary blood flow may be accomplished by the smooth muscle-related pre- and postcapillary pericytes whereas the nonmuscle pericytes of true capillaries may play a role in other functions.

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Selected References

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  1. Antonelli-Orlidge A., Saunders K. B., Smith S. R., D'Amore P. A. An activated form of transforming growth factor beta is produced by cocultures of endothelial cells and pericytes. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4544–4548. doi: 10.1073/pnas.86.12.4544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Drenckhahn D., Dermietzel R. Organization of the actin filament cytoskeleton in the intestinal brush border: a quantitative and qualitative immunoelectron microscope study. J Cell Biol. 1988 Sep;107(3):1037–1048. doi: 10.1083/jcb.107.3.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Drenckhahn D., Gröschel-Stewart U. Localization of myosin, actin, and tropomyosin in rat intestinal epithelium: immunohistochemical studies at the light and electron microscope levels. J Cell Biol. 1980 Aug;86(2):475–482. doi: 10.1083/jcb.86.2.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Drenckhahn D., Wagner J. Stress fibers in the splenic sinus endothelium in situ: molecular structure, relationship to the extracellular matrix, and contractility. J Cell Biol. 1986 May;102(5):1738–1747. doi: 10.1083/jcb.102.5.1738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fujimoto T., Singer S. J. Immunocytochemical studies of desmin and vimentin in pericapillary cells of chicken. J Histochem Cytochem. 1987 Oct;35(10):1105–1115. doi: 10.1177/35.10.3305702. [DOI] [PubMed] [Google Scholar]
  6. Gabbiani G., Kocher O., Bloom W. S., Vandekerckhove J., Weber K. Actin expression in smooth muscle cells of rat aortic intimal thickening, human atheromatous plaque, and cultured rat aortic media. J Clin Invest. 1984 Jan;73(1):148–152. doi: 10.1172/JCI111185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herman I. M., D'Amore P. A. Microvascular pericytes contain muscle and nonmuscle actins. J Cell Biol. 1985 Jul;101(1):43–52. doi: 10.1083/jcb.101.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Joyce N. C., DeCamilli P., Boyles J. Pericytes, like vascular smooth muscle cells, are immunocytochemically positive for cyclic GMP-dependent protein kinase. Microvasc Res. 1984 Sep;28(2):206–219. doi: 10.1016/0026-2862(84)90018-9. [DOI] [PubMed] [Google Scholar]
  9. Joyce N. C., Haire M. F., Palade G. E. Contractile proteins in pericytes. I. Immunoperoxidase localization of tropomyosin. J Cell Biol. 1985 May;100(5):1379–1386. doi: 10.1083/jcb.100.5.1379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Joyce N. C., Haire M. F., Palade G. E. Contractile proteins in pericytes. II. Immunocytochemical evidence for the presence of two isomyosins in graded concentrations. J Cell Biol. 1985 May;100(5):1387–1395. doi: 10.1083/jcb.100.5.1387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KUWABARA T., COGAN D. G. Retinal vascular patterns. VI. Mural cells of the retinal capillaries. Arch Ophthalmol. 1963 Apr;69:492–502. doi: 10.1001/archopht.1963.00960040498013. [DOI] [PubMed] [Google Scholar]
  12. KUWABARA T., COGAN D. G. Tetrazolium studies on the retina. I. Introduction and technique. J Histochem Cytochem. 1959 Sep;7:329–333. doi: 10.1177/7.5.329. [DOI] [PubMed] [Google Scholar]
  13. Kelley C., D'Amore P., Hechtman H. B., Shepro D. Microvascular pericyte contractility in vitro: comparison with other cells of the vascular wall. J Cell Biol. 1987 Mar;104(3):483–490. doi: 10.1083/jcb.104.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Larson D. M., Fujiwara K., Alexander R. W., Gimbrone M. A., Jr Heterogeneity of myosin antigenic expression in vascular smooth muscle in vivo. Lab Invest. 1984 Apr;50(4):401–407. [PubMed] [Google Scholar]
  15. Meyrick B., Reid L. Pulmonary hypertension. Anatomic and physiologic correlates. Clin Chest Med. 1983 May;4(2):199–217. [PubMed] [Google Scholar]
  16. Nakane P. K. Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat. J Histochem Cytochem. 1968 Sep;16(9):557–560. doi: 10.1177/16.9.557. [DOI] [PubMed] [Google Scholar]
  17. Orlidge A., D'Amore P. A. Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells. J Cell Biol. 1987 Sep;105(3):1455–1462. doi: 10.1083/jcb.105.3.1455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Skalli O., Pelte M. F., Peclet M. C., Gabbiani G., Gugliotta P., Bussolati G., Ravazzola M., Orci L. Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes. J Histochem Cytochem. 1989 Mar;37(3):315–321. doi: 10.1177/37.3.2918221. [DOI] [PubMed] [Google Scholar]
  19. Skalli O., Ropraz P., Trzeciak A., Benzonana G., Gillessen D., Gabbiani G. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol. 1986 Dec;103(6 Pt 2):2787–2796. doi: 10.1083/jcb.103.6.2787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Skalli O., Vandekerckhove J., Gabbiani G. Actin-isoform pattern as a marker of normal or pathological smooth-muscle and fibroblastic tissues. Differentiation. 1987;33(3):232–238. doi: 10.1111/j.1432-0436.1987.tb01562.x. [DOI] [PubMed] [Google Scholar]
  21. Tilton R. G., Kilo C., Williamson J. R., Murch D. W. Differences in pericyte contractile function in rat cardiac and skeletal muscle microvasculatures. Microvasc Res. 1979 Nov;18(3):336–352. doi: 10.1016/0026-2862(79)90042-6. [DOI] [PubMed] [Google Scholar]
  22. Tontsch U., Bauer H. C. Isolation, characterization, and long-term cultivation of porcine and murine cerebral capillary endothelial cells. Microvasc Res. 1989 Mar;37(2):148–161. doi: 10.1016/0026-2862(89)90034-4. [DOI] [PubMed] [Google Scholar]

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