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. 1996 Apr;34(4):792–797. doi: 10.1128/jcm.34.4.792-797.1996

Development and application of enzyme-linked immunosorbent assay for specific detection of Salmonella enteritidis infections in chickens based on antibodies to SEF14 fimbrial antigen.

C J Thorns 1, M M Bell 1, M G Sojka 1, R A Nicholas 1
PMCID: PMC228894  PMID: 8815085

Abstract

A double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to the SEF14 fimbrial antigen (SEF14-DAS ELISA) and was evaluated for its use in the specific detection of chicken flocks infected with Salmonella enteritidis. The SEF14-DAS ELISA successfully discriminated between chickens experimentally infected with S. enteritidis and those infected with S. panama or S. typhimurium, although the SEF14 responses in adult birds infected with S. enteritidis were detectable but low. In contrast, ELISAs used to detect antibodies to lipopolysaccharide (LPS) and flagella were unable to discriminate between the infected groups of chicks and adult birds infected with different Salmonella serotypes. LPS and flagellar responses were low and variable in chicks, whereas in adult hens they were found to be consistently strong. When flocks naturally infected with S. enteritidis were tested by the SEF14-DAS ELISA and ELISAs to detect LPS and flagellar antibodies, it was found that they could all identify the infected flocks, although there was little correlation between individual serum samples. The study shows that the SEF14-DAS ELISA may offer advantages over existing assays with comparable sensitivities coupled with higher specificities for the serological detection of S. enteritidis-infected chicken flocks.

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Selected References

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  1. Barrow P. A. ELISAs and the serological analysis of Salmonella infections in poultry: a review. Epidemiol Infect. 1992 Dec;109(3):361–369. doi: 10.1017/s0950268800050354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barrow P. A. Serological diagnosis of Salmonella serotype enteritidis infections in poultry by ELISA and other tests. Int J Food Microbiol. 1994 Jan;21(1-2):55–68. doi: 10.1016/0168-1605(94)90200-3. [DOI] [PubMed] [Google Scholar]
  3. Cooper G. L., Venables L. M., Nicholas R. A., Cullen G. A., Hormaeche C. E. Vaccination of chickens with chicken-derived Salmonella enteritidis phage type 4 aroA live oral Salmonella vaccines. Vaccine. 1992;10(4):247–254. doi: 10.1016/0264-410x(92)90160-l. [DOI] [PubMed] [Google Scholar]
  4. Hassan J. O., Barrow P. A., Mockett A. P., Mcleod S. Antibody response to experimental Salmonella typhimurium infection in chickens measured by ELISA. Vet Rec. 1990 May 26;126(21):519–522. [PubMed] [Google Scholar]
  5. Kim C. J., Nagaraja K. V., Pomeroy B. S. Enzyme-linked immunosorbent assay for the detection of Salmonella enteritidis infection in chickens. Am J Vet Res. 1991 Jul;52(7):1069–1074. [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Müller K. H., Collinson S. K., Trust T. J., Kay W. W. Type 1 fimbriae of Salmonella enteritidis. J Bacteriol. 1991 Aug;173(15):4765–4772. doi: 10.1128/jb.173.15.4765-4772.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nicholas R. A., Cullen G. A. Development and application of an ELISA for detecting antibodies to Salmonella enteritidis in chicken flocks. Vet Rec. 1991 Jan 26;128(4):74–76. doi: 10.1136/vr.128.4.74. [DOI] [PubMed] [Google Scholar]
  9. O'Brien J. D. Salmonella enteritidis infection in broiler chickens. Vet Rec. 1988 Feb 27;122(9):214–214. doi: 10.1136/vr.122.9.214-b. [DOI] [PubMed] [Google Scholar]
  10. Rodrigue D. C., Tauxe R. V., Rowe B. International increase in Salmonella enteritidis: a new pandemic? Epidemiol Infect. 1990 Aug;105(1):21–27. doi: 10.1017/s0950268800047609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. St Louis M. E., Morse D. L., Potter M. E., DeMelfi T. M., Guzewich J. J., Tauxe R. V., Blake P. A. The emergence of grade A eggs as a major source of Salmonella enteritidis infections. New implications for the control of salmonellosis. JAMA. 1988 Apr 8;259(14):2103–2107. [PubMed] [Google Scholar]
  12. Thorns C. J., McLaren I. M., Sojka M. G. The use of latex particle agglutination to specifically detect Salmonella enteritidis. Int J Food Microbiol. 1994 Jan;21(1-2):47–53. doi: 10.1016/0168-1605(94)90199-6. [DOI] [PubMed] [Google Scholar]
  13. Thorns C. J., Sojka M. G., Chasey D. Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using a monoclonal antibody. J Clin Microbiol. 1990 Nov;28(11):2409–2414. doi: 10.1128/jcm.28.11.2409-2414.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thorns C. J., Sojka M. G., Mclaren I. M., Dibb-Fuller M. Characterisation of monoclonal antibodies against a fimbrial structure of Salmonella enteritidis and certain other serogroup D salmonellae and their application as serotyping reagents. Res Vet Sci. 1992 Nov;53(3):300–308. doi: 10.1016/0034-5288(92)90130-t. [DOI] [PubMed] [Google Scholar]
  15. Timms L. M., Marshall R. N., Breslin M. F. Laboratory and field trial assessment of protection given by a Salmonella enteritidis PT4 inactivated, adjuvant vaccine. Br Vet J. 1994 Jan-Feb;150(1):93–102. doi: 10.1016/S0007-1935(05)80101-0. [DOI] [PubMed] [Google Scholar]
  16. Timoney J. F., Sikora N., Shivaprasad H. L., Opitz M. Detection of antibody to Salmonella enteritidis by a gm flagellin-based ELISA. Vet Rec. 1990 Aug 18;127(7):168–169. [PubMed] [Google Scholar]
  17. Williams J. E., Whittemore A. D. Influence of age on the serological response of chickens to Salmonella typhimurium infection. Avian Dis. 1975 Oct-Dec;19(4):745–760. [PubMed] [Google Scholar]
  18. Williams J. E., Whittenmore A. D. Comparison of six methods of detecting Salmonella typhimurium infection of chickens. Avian Dis. 1976 Oct-Dec;20(4):728–734. [PubMed] [Google Scholar]
  19. van Zijderveld F. G., van Zijderveld-van Bemmel A. M., Anakotta J. Comparison of four different enzyme-linked immunosorbent assays for serological diagnosis of Salmonella enteritidis infections in experimentally infected chickens. J Clin Microbiol. 1992 Oct;30(10):2560–2566. doi: 10.1128/jcm.30.10.2560-2566.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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