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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1996 Apr;34(4):853–859. doi: 10.1128/jcm.34.4.853-859.1996

Genetic relatedness within and between serotypes of Streptococcus pneumoniae from the United Kingdom: analysis of multilocus enzyme electrophoresis, pulsed-field gel electrophoresis, and antimicrobial resistance patterns.

L M Hall 1, R A Whiley 1, B Duke 1, R C George 1, A Efstratiou 1
PMCID: PMC228905  PMID: 8815096

Abstract

A collection of 54 isolates of invasive Streptococcus pneumoniae of serotypes 3 and 14 and serogroups 6, 9, 19, and 23 was investigated. Multilocus enzyme electrophoresis and pulsed-field gel electrophoresis suggested that two clones were represented in the collection, one of serotype 14 isolates, most of which were resistant to erythromycin, and one of serotype 9V isolates, in which resistance to penicillin (MIC, 1 microgram/ml), cefotaxime, and co-trimoxazole was common. Among other isolates there were only a limited correlation between genetic relatedness measured by multilocus enzyme electrophoresis and expression of the same capsule type. However, isolates with highly related pulsed-field gel electrophoresis patterns always shared the same serotype and highly related allele profiles. Calculation of the index of association suggests a freely recombining population structure with epidemic spread of successful clones.

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Selected References

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  1. Aszkenasy O. M., George R. C., Begg N. T. Pneumococcal bacteraemia and meningitis in England and Wales 1982 to 1992. Commun Dis Rep CDR Rev. 1995 Mar 31;5(4):R45–R50. [PubMed] [Google Scholar]
  2. Boulnois G. J. Pneumococcal proteins and the pathogenesis of disease caused by Streptococcus pneumoniae. J Gen Microbiol. 1992 Feb;138(2):249–259. doi: 10.1099/00221287-138-2-249. [DOI] [PubMed] [Google Scholar]
  3. Coffey T. J., Dowson C. G., Daniels M., Zhou J., Martin C., Spratt B. G., Musser J. M. Horizontal transfer of multiple penicillin-binding protein genes, and capsular biosynthetic genes, in natural populations of Streptococcus pneumoniae. Mol Microbiol. 1991 Sep;5(9):2255–2260. doi: 10.1111/j.1365-2958.1991.tb02155.x. [DOI] [PubMed] [Google Scholar]
  4. Figueiredo A. M., Austrian R., Urbaskova P., Teixeira L. A., Tomasz A. Novel penicillin-resistant clones of Streptococcus pneumoniae in the Czech Republic and in Slovakia. Microb Drug Resist. 1995 Spring;1(1):71–78. doi: 10.1089/mdr.1995.1.71. [DOI] [PubMed] [Google Scholar]
  5. Gasc A. M., Geslin P., Sicard A. M. Relatedness of penicillin-resistant Streptococcus pneumoniae serogroup 9 strains from France and Spain. Microbiology. 1995 Mar;141(Pt 3):623–627. doi: 10.1099/13500872-141-3-623. [DOI] [PubMed] [Google Scholar]
  6. Hall L. M. Are point mutations or DNA rearrangements responsible for the restriction fragment length polymorphisms that are used to type bacteria? Microbiology. 1994 Jan;140(Pt 1):197–204. doi: 10.1099/13500872-140-1-197. [DOI] [PubMed] [Google Scholar]
  7. Hermans P. W., Sluijter M., Hoogenboezem T., Heersma H., van Belkum A., de Groot R. Comparative study of five different DNA fingerprint techniques for molecular typing of Streptococcus pneumoniae strains. J Clin Microbiol. 1995 Jun;33(6):1606–1612. doi: 10.1128/jcm.33.6.1606-1612.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kell C. M., Jordens J. Z., Daniels M., Coffey T. J., Bates J., Paul J., Gilks C., Spratt B. G. Molecular epidemiology of penicillin-resistant pneumococci isolated in Nairobi, Kenya. Infect Immun. 1993 Oct;61(10):4382–4391. doi: 10.1128/iai.61.10.4382-4391.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kelly T., Dillard J. P., Yother J. Effect of genetic switching of capsular type on virulence of Streptococcus pneumoniae. Infect Immun. 1994 May;62(5):1813–1819. doi: 10.1128/iai.62.5.1813-1819.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klugman K. P. Pneumococcal resistance to antibiotics. Clin Microbiol Rev. 1990 Apr;3(2):171–196. doi: 10.1128/cmr.3.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lefevre J. C., Faucon G., Sicard A. M., Gasc A. M. DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis. J Clin Microbiol. 1993 Oct;31(10):2724–2728. doi: 10.1128/jcm.31.10.2724-2728.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lefèvre J. C., Bertrand M. A., Faucon G. Molecular analysis by pulsed-field gel electrophoresis of penicillin-resistant Streptococcus pneumoniae from Toulouse, France. Eur J Clin Microbiol Infect Dis. 1995 Jun;14(6):491–497. doi: 10.1007/BF02113426. [DOI] [PubMed] [Google Scholar]
  13. McDougal L. K., Facklam R., Reeves M., Hunter S., Swenson J. M., Hill B. C., Tenover F. C. Analysis of multiply antimicrobial-resistant isolates of Streptococcus pneumoniae from the United States. Antimicrob Agents Chemother. 1992 Oct;36(10):2176–2184. doi: 10.1128/aac.36.10.2176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mogdasy M. C., Camou T., Fajardo C., Hortal M. Colonizing and invasive strains of Streptococcus pneumoniae in Uruguayan children: type distribution and patterns of antibiotic resistance. Pediatr Infect Dis J. 1992 Aug;11(8):648–652. [PubMed] [Google Scholar]
  15. Munoz R., Musser J. M., Crain M., Briles D. E., Marton A., Parkinson A. J., Sorensen U., Tomasz A. Geographic distribution of penicillin-resistant clones of Streptococcus pneumoniae: characterization by penicillin-binding protein profile, surface protein A typing, and multilocus enzyme analysis. Clin Infect Dis. 1992 Jul;15(1):112–118. doi: 10.1093/clinids/15.1.112. [DOI] [PubMed] [Google Scholar]
  16. Muñoz R., Coffey T. J., Daniels M., Dowson C. G., Laible G., Casal J., Hakenbeck R., Jacobs M., Musser J. M., Spratt B. G. Intercontinental spread of a multiresistant clone of serotype 23F Streptococcus pneumoniae. J Infect Dis. 1991 Aug;164(2):302–306. doi: 10.1093/infdis/164.2.302. [DOI] [PubMed] [Google Scholar]
  17. O'Rourke M., Stevens E. Genetic structure of Neisseria gonorrhoeae populations: a non-clonal pathogen. J Gen Microbiol. 1993 Nov;139(11):2603–2611. doi: 10.1099/00221287-139-11-2603. [DOI] [PubMed] [Google Scholar]
  18. Parkinson A. J., Davidson M., Fitzgerald M. A., Bulkow L. R., Parks D. J. Serotype distribution and antimicrobial resistance patterns of invasive isolates of Streptococcus pneumoniae: Alaska 1986-1990. J Infect Dis. 1994 Aug;170(2):461–464. doi: 10.1093/infdis/170.2.461. [DOI] [PubMed] [Google Scholar]
  19. Robbins J. B., Austrian R., Lee C. J., Rastogi S. C., Schiffman G., Henrichsen J., Mäkelä P. H., Broome C. V., Facklam R. R., Tiesjema R. H. Considerations for formulating the second-generation pneumococcal capsular polysaccharide vaccine with emphasis on the cross-reactive types within groups. J Infect Dis. 1983 Dec;148(6):1136–1159. doi: 10.1093/infdis/148.6.1136. [DOI] [PubMed] [Google Scholar]
  20. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shibl A. M., Hussein S. S. Surveillance of Streptococcus pneumoniae serotypes in Riyadh and their susceptibility to penicillin and other commonly prescribed antibiotics. J Antimicrob Chemother. 1992 Feb;29(2):149–157. doi: 10.1093/jac/29.2.149. [DOI] [PubMed] [Google Scholar]
  22. Sibold C., Wang J., Henrichsen J., Hakenbeck R. Genetic relationships of penicillin-susceptible and -resistant Streptococcus pneumoniae strains isolated on different continents. Infect Immun. 1992 Oct;60(10):4119–4126. doi: 10.1128/iai.60.10.4119-4126.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith J. M., Smith N. H., O'Rourke M., Spratt B. G. How clonal are bacteria? Proc Natl Acad Sci U S A. 1993 May 15;90(10):4384–4388. doi: 10.1073/pnas.90.10.4384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Soares S., Kristinsson K. G., Musser J. M., Tomasz A. Evidence for the introduction of a multiresistant clone of serotype 6B Streptococcus pneumoniae from Spain to Iceland in the late 1980s. J Infect Dis. 1993 Jul;168(1):158–163. doi: 10.1093/infdis/168.1.158. [DOI] [PubMed] [Google Scholar]
  25. Urwin G., Yuan M. F., Feldman R. A. Prospective study of bacterial meningitis in North East Thames region, 1991-3, during introduction of Haemophilus influenzae vaccine. BMJ. 1994 Nov 26;309(6966):1412–1414. doi: 10.1136/bmj.309.6966.1412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Versalovic J., Kapur V., Mason E. O., Jr, Shah U., Koeuth T., Lupski J. R., Musser J. M. Penicillin-resistant Streptococcus pneumoniae strains recovered in Houston: identification and molecular characterization of multiple clones. J Infect Dis. 1993 Apr;167(4):850–856. doi: 10.1093/infdis/167.4.850. [DOI] [PubMed] [Google Scholar]
  27. Viering T. P., Fine D. P. Genetic analysis of Streptococcus pneumoniae serotypes with the use of DNA fingerprinting. J Infect Dis. 1989 Jul;160(1):76–82. doi: 10.1093/infdis/160.1.76. [DOI] [PubMed] [Google Scholar]
  28. Wenger J. D., Hightower A. W., Facklam R. R., Gaventa S., Broome C. V. Bacterial meningitis in the United States, 1986: report of a multistate surveillance study. The Bacterial Meningitis Study Group. J Infect Dis. 1990 Dec;162(6):1316–1323. doi: 10.1093/infdis/162.6.1316. [DOI] [PubMed] [Google Scholar]

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