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. 1996 Apr;34(4):983–985. doi: 10.1128/jcm.34.4.983-985.1996

Serum rotavirus neutralizing-antibody titers compared by plaque reduction and enzyme-linked immunosorbent assay-based neutralization assays.

R L Ward 1, A Z Kapikian 1, K M Goldberg 1, D R Knowlton 1, M W Watson 1, R Rappaport 1
PMCID: PMC228933  PMID: 8815124

Abstract

Comparisons in rotavirus neutralizing-antibody responses were made with sera collected from vaccinated infants. The methods were a plaque reduction assay and a new enzyme-linked immunosorbent assay-based neutralization assay. Agreement of 94% was found in detecting at least fourfold seroresponses, and correlation coefficients between titers obtained by the two methods showed excellent agreement, indicating that either could be used reliably.

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Selected References

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  1. Bernstein D. I., Kacica M. A., McNeal M. M., Schiff G. M., Ward R. L. Local and systemic antibody response to rotavirus WC3 vaccine in adult volunteers. Antiviral Res. 1989 Dec;12(5-6):293–300. doi: 10.1016/0166-3542(89)90056-9. [DOI] [PubMed] [Google Scholar]
  2. Bernstein D. I., Smith V. E., Sander D. S., Pax K. A., Schiff G. M., Ward R. L. Evaluation of WC3 rotavirus vaccine and correlates of protection in healthy infants. J Infect Dis. 1990 Nov;162(5):1055–1062. doi: 10.1093/infdis/162.5.1055. [DOI] [PubMed] [Google Scholar]
  3. Bridger J. C., Woode G. N. Neonatal calf diarrhoea: identification of a reovirus-like (rotavirus) agent in faeces by immunofluorescence and immune electron microscopy. Br Vet J. 1975 Sep-Oct;131(5):528–535. [PubMed] [Google Scholar]
  4. Clark H. F., Borian F. E., Plotkin S. A. Immune protection of infants against rotavirus gastroenteritis by a serotype 1 reassortant of bovine rotavirus WC3. J Infect Dis. 1990 Jun;161(6):1099–1104. doi: 10.1093/infdis/161.6.1099. [DOI] [PubMed] [Google Scholar]
  5. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flores J., Perez-Schael I., Blanco M., Rojas A. M., Alfonzo E., Crespo I., Cunto W., Pittman A. L., Kapikian A. Z. Reactogenicity and immunogenicity of a high-titer rhesus rotavirus-based quadrivalent rotavirus vaccine. J Clin Microbiol. 1993 Sep;31(9):2439–2445. doi: 10.1128/jcm.31.9.2439-2445.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gorziglia M., Larralde G., Kapikian A. Z., Chanock R. M. Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci U S A. 1990 Sep;87(18):7155–7159. doi: 10.1073/pnas.87.18.7155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoshino Y., Kapikian A. Z. Rotavirus vaccine development for the prevention of severe diarrhea in infants and young children. Trends Microbiol. 1994 Jul;2(7):242–249. doi: 10.1016/0966-842x(94)90629-7. [DOI] [PubMed] [Google Scholar]
  9. Knowlton D. R., Spector D. M., Ward R. L. Development of an improved method for measuring neutralizing antibody to rotavirus. J Virol Methods. 1991 Jun;33(1-2):127–134. doi: 10.1016/0166-0934(91)90013-p. [DOI] [PubMed] [Google Scholar]
  10. Midthun K., Greenberg H. B., Hoshino Y., Kapikian A. Z., Wyatt R. G., Chanock R. M. Reassortant rotaviruses as potential live rotavirus vaccine candidates. J Virol. 1985 Mar;53(3):949–954. doi: 10.1128/jvi.53.3.949-954.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Midthun K., Hoshino Y., Kapikian A. Z., Chanock R. M. Single gene substitution rotavirus reassortants containing the major neutralization protein (VP7) of human rotavirus serotype 4. J Clin Microbiol. 1986 Nov;24(5):822–826. doi: 10.1128/jcm.24.5.822-826.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rennels M. B., Glass R. I., Dennehy P. H., Bernstein D. I., Pichichero M. E., Zito E. T., Mack M. E., Davidson B. L., Kapikian A. Z. Safety and efficacy of high-dose rhesus-human reassortant rotavirus vaccines--report of the National Multicenter Trial. United States Rotavirus Vaccine Efficacy Group. Pediatrics. 1996 Jan;97(1):7–13. [PubMed] [Google Scholar]
  13. Silberstein I., Shulman L. M., Mendelson E., Shif I. Distribution of both rotavirus VP4 genotypes and VP7 serotypes among hospitalized and nonhospitalized Israeli children. J Clin Microbiol. 1995 May;33(5):1421–1422. doi: 10.1128/jcm.33.5.1421-1422.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Steele A. D., van Niekerk M. C., Mphahlele M. J. Geographic distribution of human rotavirus VP4 genotypes and VP7 serotypes in five South African regions. J Clin Microbiol. 1995 Jun;33(6):1516–1519. doi: 10.1128/jcm.33.6.1516-1519.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Timenetsky M. do C., Santos N., Gouvea V. Survey of rotavirus G and P types associated with human gastroenteritis in São Paulo, Brazil, from 1986 to 1992. J Clin Microbiol. 1994 Oct;32(10):2622–2624. doi: 10.1128/jcm.32.10.2622-2624.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vesikari T., Isolauri E., Delem A., D'Hondt E., André F. E., Zissis G. Immunogenicity and safety of live oral attenuated bovine rotavirus vaccine strain RIT 4237 in adults and young children. Lancet. 1983 Oct 8;2(8354):807–811. doi: 10.1016/s0140-6736(83)90734-1. [DOI] [PubMed] [Google Scholar]
  17. Ward R. L., Bernstein D. I. Lack of correlation between serum rotavirus antibody titers and protection following vaccination with reassortant RRV vaccines. US Rotavirus Vaccine Efficacy Group. Vaccine. 1995 Sep;13(13):1226–1232. doi: 10.1016/0264-410x(95)00060-e. [DOI] [PubMed] [Google Scholar]
  18. Wyatt R. G., James H. D., Jr, Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. J Clin Microbiol. 1983 Aug;18(2):310–317. doi: 10.1128/jcm.18.2.310-317.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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