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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1996 Apr;34(4):986–987. doi: 10.1128/jcm.34.4.986-987.1996

Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA.

A J da Silva 1, D A Schwartz 1, G S Visvesvara 1, H de Moura 1, S B Slemenda 1, N J Pieniazek 1
PMCID: PMC228934  PMID: 8815125

Abstract

Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.

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Selected References

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