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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1996 Apr;34(4):999–1002. doi: 10.1128/jcm.34.4.999-1002.1996

Performance characteristics of recombinant enzyme immunoassay to detect antibodies to human immunodeficiency virus type 1 (HIV-1) and HIV-2 and to measure early antibody responses in seroconverting patients.

R A Galli 1, S Castriciano 1, M Fearon 1, C Major 1, K W Choi 1, J Mahony 1, M Chernesky 1
PMCID: PMC228939  PMID: 8815130

Abstract

We investigated the performance of a double-antigen sandwich recombinant enzyme immunoassay (EIA; Abbott Laboratories, North Chicago, Ill.) and compared it with that of a synthetic-peptide-based EIA (Biochem Immunosystems, Montreal, Quebec, Canada) for the detection of human immunodeficiency type 1 (HIV-1) and HIV-2 antibodies in 2,321 clinical serum samples. The results of both EIA methods and Western blot (immunoblot) were in agreement for 1,046 HIV-1 and 10 HIV-2 specimens from a panel of known positives. From a prospective panel of 1,085 specimens, 38 proved to be positive by both EIAs and Western blot, 3 were positive by the recombinant EIA only, and 9 were positive by the peptide EIA only, for calculated specificities of 99.71 and 99.04%, respectively. Of 180 specimens from a seroconversion panel collected from 77 patients, the results for 170 were in agreement by all antibody testing methods and 10 were found to be repeat reactive for HIV antibodies by the recombinant EIA only. All 10 were initial specimens of seroconverting patients; 7 were also reactive for HIV p24 antigen. An examination of four of these sera by radioimmunoprecipitation assay showed gp120 and gp160 bands in each. Analysis of the anti-Env antibody class in three of these samples showed that one consisted of immunoglobulin M (IgM) only and two contained both IgG and IgM antibodies. Although both EIA procedures were sensitive and specific in the detection of antibodies to HIV-1 and HIV-2 and both were capable of detecting early antibodies, the recombinant assay was more sensitive for antibody detection during early seroconversion.

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Selected References

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  1. Albert J., Bredberg U., Chiodi F., Böttiger B., Fenyö E. M., Norrby E., Biberfeld G. A new human retrovirus isolate of West African origin (SBL-6669) and its relationship to HTLV-IV, LAV-II, and HTLV-IIIB. AIDS Res Hum Retroviruses. 1987 Spring;3(1):3–10. doi: 10.1089/aid.1987.3.3. [DOI] [PubMed] [Google Scholar]
  2. Busch M. P., Lee L. L., Satten G. A., Henrard D. R., Farzadegan H., Nelson K. E., Read S., Dodd R. Y., Petersen L. R. Time course of detection of viral and serologic markers preceding human immunodeficiency virus type 1 seroconversion: implications for screening of blood and tissue donors. Transfusion. 1995 Feb;35(2):91–97. doi: 10.1046/j.1537-2995.1995.35295125745.x. [DOI] [PubMed] [Google Scholar]
  3. Busch M. P., Petersen L., Schable C., Perkins H. Monitoring blood donors for HIV-2 infection by testing anti-HIV-1 reactive sera. Transfusion. 1990 Feb;30(2):184–187. doi: 10.1046/j.1537-2995.1990.30290162908.x. [DOI] [PubMed] [Google Scholar]
  4. De Cock K. M., Porter A., Kouadio J., Maran M., Gnaore E., Adjorlolo G., Lafontaine M. F., Bretton G., Damet G. M., Odehouri K. Rapid and specific diagnosis of HIV-1 and HIV-2 infections: an evaluation of testing strategies. AIDS. 1990 Sep;4(9):875–878. doi: 10.1097/00002030-199009000-00007. [DOI] [PubMed] [Google Scholar]
  5. Gallarda J. L., Henrard D. R., Liu D., Harrington S., Stramer S. L., Valinsky J. E., Wu P. Early detection of antibody to human immunodeficiency virus type 1 by using an antigen conjugate immunoassay correlates with the presence of immunoglobulin M antibody. J Clin Microbiol. 1992 Sep;30(9):2379–2384. doi: 10.1128/jcm.30.9.2379-2384.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guyader M., Emerman M., Sonigo P., Clavel F., Montagnier L., Alizon M. Genome organization and transactivation of the human immunodeficiency virus type 2. Nature. 1987 Apr 16;326(6114):662–669. doi: 10.1038/326662a0. [DOI] [PubMed] [Google Scholar]
  7. Holloman D. L., Pau C. P., Parekh B., Schable C., Onorato I., Schochetman G., George J. R. Evaluation of testing algorithms following the use of combination HIV-1/HIV-2 EIA for screening purposes. AIDS Res Hum Retroviruses. 1993 Feb;9(2):147–151. doi: 10.1089/aid.1993.9.147. [DOI] [PubMed] [Google Scholar]
  8. Johnson J. E. Detection of human immunodeficiency virus type 1 antibody by using commercially available whole-cell viral lysate, synthetic peptide, and recombinant protein enzyme immunoassay systems. J Clin Microbiol. 1992 Jan;30(1):216–218. doi: 10.1128/jcm.30.1.216-218.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lange J. M., Parry J. V., de Wolf F., Mortimer P. P., Goudsmit J. Diagnostic value of specific IgM antibodies in primary HIV infection. AIDS. 1988 Feb;2(1):31–35. doi: 10.1097/00002030-198802000-00005. [DOI] [PubMed] [Google Scholar]
  10. Neumann P. W., Benning B. J., Robinson D. G., Gilmore N., O'Shaughnessy M. V. HTLV-III infection in Canada in 1985. CMAJ. 1986 Sep 1;135(5):477–480. [PMC free article] [PubMed] [Google Scholar]
  11. Neumann P. W., O'Shaughnessy M. V., Lepine D., D'Souza I., Major C., McLaughlin B. Laboratory diagnosis of the first cases of HIV-2 infection in Canada. CMAJ. 1989 Jan 15;140(2):125–128. [PMC free article] [PubMed] [Google Scholar]
  12. O'Brien T. R., George J. R., Holmberg S. D. Human immunodeficiency virus type 2 infection in the United States. Epidemiology, diagnosis, and public health implications. JAMA. 1992 May 27;267(20):2775–2779. [PubMed] [Google Scholar]
  13. Palomba E., Gay V., de Martino M., Fundaró C., Perugini L., Tovo P. A. Early diagnosis of human immunodeficiency virus infection in infants by detection of free and complexed p24 antigen. J Infect Dis. 1992 Feb;165(2):394–395. doi: 10.1093/infdis/165.2.394. [DOI] [PubMed] [Google Scholar]
  14. Parry J. V., McAlpine L., Avillez M. F. Sensitivity of six commercial enzyme immunoassay kits that detect both anti-HIV-1 and anti-HIV-2. AIDS. 1990 Apr;4(4):355–360. doi: 10.1097/00002030-199004000-00012. [DOI] [PubMed] [Google Scholar]
  15. Smith R. S., Parks E. Synthetic peptide assays to detect human immunodeficiency virus types 1 and 2 in seropositive individuals. Arch Pathol Lab Med. 1990 Mar;114(3):254–258. [PubMed] [Google Scholar]
  16. Sprengers E. D., Hellings J. A., Theunissen H. J., Ten Kortenaar P. B. A microELISA system for the detection of both anti-HIV-1 and anti-HIV-2 antibodies. J Immunol Methods. 1991 May 17;139(1):77–82. doi: 10.1016/0022-1759(91)90353-h. [DOI] [PubMed] [Google Scholar]
  17. Zaaijer H. L., v Exel-Oehlers P., Kraaijeveld T., Altena E., Lelie P. N. Early detection of antibodies to HIV-1 by third-generation assays. Lancet. 1992 Sep 26;340(8822):770–772. doi: 10.1016/0140-6736(92)92303-w. [DOI] [PubMed] [Google Scholar]

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