Abstract
The immunoglobulin A-specific reactivities of recombinant viral proteins from nine different reading frames of human cytomegalovirus were evaluated in enzyme-linked immunosorbent assay experiments. Antigen fragments of reading frames pUL32, pUL44, and pUL57 were identified as preferable antigens for immunoglobulin A serodiagnosis. Application of autologous fusion proteins which combine these polypeptides may be useful especially for the early detection of acute secondary human cytomegalovirus infection.
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- Braun W., Weber B., Moell U., Hamann A., Doerr H. W. Immunoglobulin A and M patterns to human cytomegalovirus during recurrent infection in patients with AIDS using a modified western blot. J Virol Methods. 1993 Jun;43(1):65–75. doi: 10.1016/0166-0934(93)90090-e. [DOI] [PubMed] [Google Scholar]
- Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A., 3rd, Kouzarides T., Martignetti J. A. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol. 1990;154:125–169. doi: 10.1007/978-3-642-74980-3_6. [DOI] [PubMed] [Google Scholar]
- Jahn G., Plachter B. Diagnostics of persistent viruses: human cytomegalovirus as an example. Intervirology. 1993;35(1-4):60–72. doi: 10.1159/000150296. [DOI] [PubMed] [Google Scholar]
- Nielsen S. L., Sørensen I., Andersen H. K. Kinetics of specific immunoglobulins M, E, A, and G in congenital, primary, and secondary cytomegalovirus infection studied by antibody-capture enzyme-linked immunosorbent assay. J Clin Microbiol. 1988 Apr;26(4):654–661. doi: 10.1128/jcm.26.4.654-661.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Noack R., Szugs C., Scholz H. Immunglobuline bei der Behandlung der bakteriellen Meningitis im Kindesalter. Infection. 1987 Jan-Feb;15(1):11–15. doi: 10.1007/BF01646110. [DOI] [PubMed] [Google Scholar]
- Porath A., Hanuka N., Keynan A., Sarov I. Virus-specific serum IgG, IgM, and IgA antibodies in cytomegalovirus mononucleosis patients as determined by immunoblotting technique. J Med Virol. 1987 Jul;22(3):223–230. doi: 10.1002/jmv.1890220305. [DOI] [PubMed] [Google Scholar]
- Sarov I., Siqueira-Linhares M., Chardonnet Y., Levy E., Aymard M., Bosshard S., Nord E., Revillard J. P. Detection of specific IgA antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection. Intervirology. 1981;15(4):228–234. doi: 10.1159/000149236. [DOI] [PubMed] [Google Scholar]
- Studier F. W., Moffatt B. A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986 May 5;189(1):113–130. doi: 10.1016/0022-2836(86)90385-2. [DOI] [PubMed] [Google Scholar]
- Vornhagen R., Hinderer W., Sonneborn H. H., Bein G., Matter L., The T. H., Jahn G., Plachter B. The DNA-binding protein pUL57 of human cytomegalovirus is a major target antigen for the immunoglobulin M antibody response during acute infection. J Clin Microbiol. 1995 Jul;33(7):1927–1930. doi: 10.1128/jcm.33.7.1927-1930.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]