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. 1996 May;34(5):1100–1107. doi: 10.1128/jcm.34.5.1100-1107.1996

Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov.

B Springer 1, W K Wu 1, T Bodmer 1, G Haase 1, G E Pfyffer 1, R M Kroppenstedt 1, K H Schröder 1, S Emler 1, J O Kilburn 1, P Kirschner 1, A Telenti 1, M B Coyle 1, E C Böttger 1
PMCID: PMC228963  PMID: 8727884

Abstract

A distinct group of slowly growing mycobacteria was identified on the basis of growth characteristics, biochemical and lipid profiles, and nucleic acid analyses. The isolates showed growth at 22 to 37 degrees C, yellow pigmentation, and negative tests for Tween 80 hydrolysis, nicotinic acid, nitrate reductase, and urease; tests for arylsulfatase, pyrazinamidase, and heat-stable catalase were variable. Analysis of cellular fatty acids by gas-liquid chromatography and mycolic acids by thin-layer chromatography and high-performance liquid chromatography indicated a distinctive pattern which was unlike those of other species. Determination of the 16S rRNA gene sequence showed a unique sequence closely related to Mycobacterium simiae and M. genavense. On the basis of DNA homology studies, we suggest that these organisms are representatives of a novel species, for which the name M. lentiflavum sp. nov. is proposed.

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Selected References

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  1. Butler W. R., Jost K. C., Jr, Kilburn J. O. Identification of mycobacteria by high-performance liquid chromatography. J Clin Microbiol. 1991 Nov;29(11):2468–2472. doi: 10.1128/jcm.29.11.2468-2472.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Butler W. R., O'Connor S. P., Yakrus M. A., Smithwick R. W., Plikaytis B. B., Moss C. W., Floyd M. M., Woodley C. L., Kilburn J. O., Vadney F. S. Mycobacterium celatum sp. nov. Int J Syst Bacteriol. 1993 Jul;43(3):539–548. doi: 10.1099/00207713-43-3-539. [DOI] [PubMed] [Google Scholar]
  3. Böttger E. C., Hirschel B., Coyle M. B. Mycobacterium genavense sp. nov. Int J Syst Bacteriol. 1993 Oct;43(4):841–843. doi: 10.1099/00207713-43-4-841. [DOI] [PubMed] [Google Scholar]
  4. Böttger E. C., Teske A., Kirschner P., Bost S., Chang H. R., Beer V., Hirschel B. Disseminated "Mycobacterium genavense" infection in patients with AIDS. Lancet. 1992 Jul 11;340(8811):76–80. doi: 10.1016/0140-6736(92)90397-l. [DOI] [PubMed] [Google Scholar]
  5. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res. 1989 Oct 11;17(19):7843–7853. doi: 10.1093/nar/17.19.7843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Haas W. H., Kirschner P., Ziesing S., Bremer H. J., Böttger E. C. Cervical lymphadenitis in a child caused by a previously unknown mycobacterium. J Infect Dis. 1993 Jan;167(1):237–240. doi: 10.1093/infdis/167.1.237. [DOI] [PubMed] [Google Scholar]
  7. Hughes M. S., Skuce R. A., Beck L. A., Neill S. D. Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences. J Clin Microbiol. 1993 Dec;31(12):3216–3222. doi: 10.1128/jcm.31.12.3216-3222.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hultman T., Ståhl S., Hornes E., Uhlén M. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 1989 Jul 11;17(13):4937–4946. doi: 10.1093/nar/17.13.4937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kazda J., Cooney R., Monaghan M., Quinn P. J., Stackebrandt E., Dorsch M., Daffé M., Müller K., Cook B. R., Tarnok Z. S. Mycobacterium hiberniae sp. nov. Int J Syst Bacteriol. 1993 Apr;43(2):352–357. doi: 10.1099/00207713-43-2-352. [DOI] [PubMed] [Google Scholar]
  10. Kazda J., Stackebrandt E., Smida J., Minnikin D. E., Daffe M., Parlett J. H., Pitulle C. Mycobacterium cookii sp. nov. Int J Syst Bacteriol. 1990 Jul;40(3):217–223. doi: 10.1099/00207713-40-3-217. [DOI] [PubMed] [Google Scholar]
  11. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Böttger E. C. Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol. 1993 Nov;31(11):2882–2889. doi: 10.1128/jcm.31.11.2882-2889.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meier A., Kirschner P., Schröder K. H., Wolters J., Kroppenstedt R. M., Böttger E. C. Mycobacterium intermedium sp. nov. Int J Syst Bacteriol. 1993 Apr;43(2):204–209. doi: 10.1099/00207713-43-2-204. [DOI] [PubMed] [Google Scholar]
  13. Rogall T., Flohr T., Böttger E. C. Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J Gen Microbiol. 1990 Sep;136(9):1915–1920. doi: 10.1099/00221287-136-9-1915. [DOI] [PubMed] [Google Scholar]
  14. Rogall T., Wolters J., Flohr T., Böttger E. C. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol. 1990 Oct;40(4):323–330. doi: 10.1099/00207713-40-4-323. [DOI] [PubMed] [Google Scholar]
  15. Sourdis J., Nei M. Relative efficiencies of the maximum parsimony and distance-matrix methods in obtaining the correct phylogenetic tree. Mol Biol Evol. 1988 May;5(3):298–311. doi: 10.1093/oxfordjournals.molbev.a040497. [DOI] [PubMed] [Google Scholar]
  16. Springer B., Kirschner P., Rost-Meyer G., Schröder K. H., Kroppenstedt R. M., Böttger E. C. Mycobacterium interjectum, a new species isolated from a patient with chronic lymphadenitis. J Clin Microbiol. 1993 Dec;31(12):3083–3089. doi: 10.1128/jcm.31.12.3083-3089.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stahl D. A., Urbance J. W. The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria. J Bacteriol. 1990 Jan;172(1):116–124. doi: 10.1128/jb.172.1.116-124.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol. 1993 Feb;31(2):175–178. doi: 10.1128/jcm.31.2.175-178.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Teske A., Wolters J., Böttger E. C. The 16S rRNA nucleotide sequence of Mycobacterium leprae: phylogenetic position and development of DNA probes. FEMS Microbiol Lett. 1991 May 15;64(2-3):231–237. doi: 10.1016/0378-1097(91)90601-6. [DOI] [PubMed] [Google Scholar]
  20. Thierry D., Vincent V., Clément F., Guesdon J. L. Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis. J Clin Microbiol. 1993 May;31(5):1048–1054. doi: 10.1128/jcm.31.5.1048-1054.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wayne L. G., Sramek H. A. Agents of newly recognized or infrequently encountered mycobacterial diseases. Clin Microbiol Rev. 1992 Jan;5(1):1–25. doi: 10.1128/cmr.5.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wolinsky E. Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis. 1979 Jan;119(1):107–159. doi: 10.1164/arrd.1979.119.1.107. [DOI] [PubMed] [Google Scholar]

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