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. 1996 May;34(5):1114–1117. doi: 10.1128/jcm.34.5.1114-1117.1996

Nontoxigenic Vibrio cholerae 01 serotype Inaba biotype El Tor associated with a cluster of cases of cholera in southern India.

P K Saha 1, H Koley 1, A K Mukhopadhyay 1, S K Bhattacharya 1, G B Nair 1, B S Ramakrishnan 1, S Krishnan 1, T Takeda 1, Y Takeda 1
PMCID: PMC228965  PMID: 8727886

Abstract

Thirteen strains of Vibrio cholerae 01 belonging to the Inaba serotype El Tor biotype isolated from patients during an outbreak of cholera in the town of Warangal in southern India were found to be nontoxigenic (NT), since they did not produce cholera toxin or hybridize with DNA probes specific for cholera toxin, Zot, or Ace. The unheated and heated culture supernatants of the NT V. cholerae 01 evoked a rapid cell-rounding effect when introduced on confluent layers of CHO and HeLa cells which could not be inhibited by antiserum against known toxins. Culture supernatants of two representative NT V. cholerae 01 strains caused an increase in short-circuit current in rabbit ileal tissue mounted on an Ussing chamber, and the pattern of increase in short-circuit current was consistent with the presence of a quickly acting toxin like stable toxin. None of the strains of NT V. cholerae 01 hybridized with a DNA probe specific for the heat-stable enterotoxin of V. cholerae non-01, nor did the factor produced by NT V. cholerae 01 resemble the recently described heat-stable enterotoxin produced by enteroaggregative Escherichia coli as determine by a PCR assay. To our knowledge, this is the first report of NT V. cholerae 01 being associated with a cluster of cases of cholera, and it appears that a clone of NT V. cholerae 01 has the potential to cause localized outbreaks of cholera.

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Selected References

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  1. Ahsan C. R., Ciznár I. Release of endotoxin by toxigenic and non-toxigenic Vibrio cholerae 01. J Diarrhoeal Dis Res. 1987 Mar;5(1):7–15. [PubMed] [Google Scholar]
  2. Almeida R. J., Cameron D. N., Cook W. L., Wachsmuth I. K. Vibriophage VcA-3 as an epidemic strain marker for the U.S. Gulf Coast Vibrio cholerae O1 clone. J Clin Microbiol. 1992 Feb;30(2):300–304. doi: 10.1128/jcm.30.2.300-304.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bhattacharya M. K., Bhattacharya S. K., Garg S., Saha P. K., Dutta D., Nair G. B., Deb B. C., Das K. P. Outbreak of Vibrio cholerae non-O1 in India and Bangladesh. Lancet. 1993 May 22;341(8856):1346–1347. doi: 10.1016/0140-6736(93)90855-b. [DOI] [PubMed] [Google Scholar]
  4. Bhattacharya S. K., Bhattacharya M. K., Nair G. B., Dutta D., Deb A., Ramamurthy T., Garg S., Saha P. K., Dutta P., Moitra A. Clinical profile of acute diarrhoea cases infected with the new epidemic strain of Vibrio cholerae O139: designation of the disease as cholera. J Infect. 1993 Jul;27(1):11–15. doi: 10.1016/0163-4453(93)93488-p. [DOI] [PubMed] [Google Scholar]
  5. Chen F., Evins G. M., Cook W. L., Almeida R., Hargrett-Bean N., Wachsmuth K. Genetic diversity among toxigenic and nontoxigenic Vibrio cholerae O1 isolated from the Western Hemisphere. Epidemiol Infect. 1991 Aug;107(1):225–233. doi: 10.1017/s0950268800048846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coelho A., Andrade J. R., Vicente A. C., Salles C. A. New variant of Vibrio cholerae O1 from clinical isolates in Amazonia. J Clin Microbiol. 1995 Jan;33(1):114–118. doi: 10.1128/jcm.33.1.114-118.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karasawa T., Mihara T., Kurazono H., Nair G. B., Garg S., Ramamurthy T., Takeda Y. Distribution of the zot (zonula occludens toxin) gene among strains of Vibrio cholerae 01 and non-01. FEMS Microbiol Lett. 1993 Jan 15;106(2):143–145. doi: 10.1111/j.1574-6968.1993.tb05950.x. [DOI] [PubMed] [Google Scholar]
  8. Kurazono H., Pal A., Bag P. K., Nair G. B., Karasawa T., Mihara T., Takeda Y. Distribution of genes encoding cholera toxin, zonula occludens toxin, accessory cholera toxin, and El Tor hemolysin in Vibrio cholerae of diverse origins. Microb Pathog. 1995 Mar;18(3):231–235. doi: 10.1016/s0882-4010(95)90076-4. [DOI] [PubMed] [Google Scholar]
  9. Moyenuddin M., Wachsmuth K., Richardson S. H., Cook W. L. Enteropathogenicity of non-toxigenic Vibrio cholerae O1 for adult mice. Microb Pathog. 1992 Jun;12(6):451–458. doi: 10.1016/0882-4010(92)90008-c. [DOI] [PubMed] [Google Scholar]
  10. Nair G. B., Oku Y., Takeda Y., Ghosh A., Ghosh R. K., Chattopadhyay S., Pal S. C., Kaper J. B., Takeda T. Toxin profiles of Vibrio cholerae non-O1 from environmental sources in Calcutta, India. Appl Environ Microbiol. 1988 Dec;54(12):3180–3182. doi: 10.1128/aem.54.12.3180-3182.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nair G. B., Ramamurthy T., Bhattacharya S. K., Mukhopadhyay A. K., Garg S., Bhattacharya M. K., Takeda T., Shimada T., Takeda Y., Deb B. C. Spread of Vibrio cholerae O139 Bengal in India. J Infect Dis. 1994 May;169(5):1029–1034. doi: 10.1093/infdis/169.5.1029. [DOI] [PubMed] [Google Scholar]
  12. Nair G. B., Shimada T., Kurazono H., Okuda J., Pal A., Karasawa T., Mihara T., Uesaka Y., Shirai H., Garg S. Characterization of phenotypic, serological, and toxigenic traits of Vibrio cholerae O139 bengal. J Clin Microbiol. 1994 Nov;32(11):2775–2779. doi: 10.1128/jcm.32.11.2775-2779.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ogawa A., Kato J., Watanabe H., Nair B. G., Takeda T. Cloning and nucleotide sequence of a heat-stable enterotoxin gene from Vibrio cholerae non-O1 isolated from a patient with traveler's diarrhea. Infect Immun. 1990 Oct;58(10):3325–3329. doi: 10.1128/iai.58.10.3325-3329.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oku Y., Uesaka Y., Hirayama T., Takeda Y. Development of a highly sensitive bead-ELISA to detect bacterial protein toxins. Microbiol Immunol. 1988;32(8):807–816. doi: 10.1111/j.1348-0421.1988.tb01442.x. [DOI] [PubMed] [Google Scholar]
  15. Ramamurthy T., Bag P. K., Pal A., Bhattacharya S. K., Bhattacharya M. K., Shimada T., Takeda T., Karasawa T., Kurazono H., Takeda Y. Virulence patterns of Vibrio cholerae non-O1 strains isolated from hospitalised patients with acute diarrhoea in Calcutta, India. J Med Microbiol. 1993 Oct;39(4):310–317. doi: 10.1099/00222615-39-4-310. [DOI] [PubMed] [Google Scholar]
  16. Ramamurthy T., Pal A., Bhattacharya M. K., Bhattacharya S. K., Chowdhury A. S., Takeda Y., Takeda T., Pal S. C., Nair G. B. Serovar, biotype, phage type, toxigenicity & antibiotic susceptibility patterns of Vibrio cholerae isolated during two consecutive cholera seasons (1989-90) in Calcutta. Indian J Med Res. 1992 May;95:125–129. [PubMed] [Google Scholar]
  17. Savarino S. J., Fasano A., Watson J., Martin B. M., Levine M. M., Guandalini S., Guerry P. Enteroaggregative Escherichia coli heat-stable enterotoxin 1 represents another subfamily of E. coli heat-stable toxin. Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):3093–3097. doi: 10.1073/pnas.90.7.3093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tacket C. O., Losonsky G., Nataro J. P., Cryz S. J., Edelman R., Fasano A., Michalski J., Kaper J. B., Levine M. M. Safety and immunogenicity of live oral cholera vaccine candidate CVD 110, a delta ctxA delta zot delta ace derivative of El Tor Ogawa Vibrio cholerae. J Infect Dis. 1993 Dec;168(6):1536–1540. doi: 10.1093/infdis/168.6.1536. [DOI] [PubMed] [Google Scholar]
  19. Takeda T., Nair G. B., Suzuki K., Shimonishi Y. Production of a monoclonal antibody to Vibrio cholerae non-O1 heat-stable enterotoxin (ST) which is cross-reactive with Yersinia enterocolitica ST. Infect Immun. 1990 Sep;58(9):2755–2759. doi: 10.1128/iai.58.9.2755-2759.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Takeda T., Peina Y., Ogawa A., Dohi S., Abe H., Nair G. B., Pal S. C. Detection of heat-stable enterotoxin in a cholera toxin gene-positive strain of Vibrio cholerae O1. FEMS Microbiol Lett. 1991 May 1;64(1):23–27. doi: 10.1016/0378-1097(91)90203-m. [DOI] [PubMed] [Google Scholar]
  21. Trucksis M., Galen J. E., Michalski J., Fasano A., Kaper J. B. Accessory cholera enterotoxin (Ace), the third toxin of a Vibrio cholerae virulence cassette. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5267–5271. doi: 10.1073/pnas.90.11.5267. [DOI] [PMC free article] [PubMed] [Google Scholar]

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