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. 2008 Mar 26;8:e16.

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Copyright © 2008 The Author(s)

This is an open-access article whereby the authors retain copyright of the work. The article is distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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NHEK cells exposed to 5 μM of SM and treated with EGF. Cells were treated daily with 0, 1, 10, or 20 ng/mL of EGF and stained with 0.1% crystal violet 6 days after wounding. For 0 μM of SM, a significant difference* (P ≤ .05) in wound fill was observed between the cells treated with 0 ng/mL of EGF and the 1, 10, and 20 ng/mL of EGF groups. For 5 μM of SM, cells treated with 1 ng/mL of EGF had the greatest percentage of wound fill, but no statistically significant differences were observed between the EGF treatment groups. Data points represent mean values ± SEM of 3 determinations. A 1-factor ANOVA was used to compare treatment groups at each SM concentration.