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. 2008 Mar 26;8:e16.

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Copyright © 2008 The Author(s)

This is an open-access article whereby the authors retain copyright of the work. The article is distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wound fill time-course study for NHEK cells exposed to various concentrations of SM and treated with 0, 10, 50, or 100 ng/mL of KGF. Cells were exposed to 0, 10, or 100 μM of SM, treated daily with the appropriate KGF concentration, and stained with 0.1% crystal violet at 6 (a) or 9 (b) days after wounding. (a) A significant difference* in percentage wound fill was observed between 0 and 10 μM of SM on day 6. No significant differences between KGF doses were observed for day 6. (b) A significant difference* in wound fill was observed between 10 and 100 μM of SM on day 9, but no significant differences were noted between the KGF doses. Data points represent mean values ± SEM of 3 determinations. A 2-factor ANOVA was used to compare the treatment groups and SM concentrations at each day, followed by a Tukey post hoc comparison of pairs of treatment groups. Statistical significance was defined as P ≤ .05 for all tests.