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. 1996 May;34(5):1189–1192. doi: 10.1128/jcm.34.5.1189-1192.1996

Molecular characterization of Vibrio cholerae O1 strains isolated during cholera outbreaks in Guinea-Bissau.

A Dalsgaard 1, H F Mortensen 1, K Mølbak 1, F Dias 1, O Serichantalergs 1, P Echeverria 1
PMCID: PMC228980  PMID: 8727901

Abstract

In the present study, 19 strains of Vibrio cholerae O1 biotype El Tor isolated during outbreaks of cholera in Guinea-Bissau in 1987, 1994, and 1995 were characterized to investigate a possible epidemiological relationship among the isolates. On the basis of ribotyping with the restriction enzyme BglI, 5 strains isolated in 1987 showed two closely related ribotypes, while 14 strains isolated in 1994 and 1995 showed the same ribotype that was distinct from the ribotypes of strains isolated in 1987. Southern blot hybridization of BglI-digested genomic DNA with a cholera toxin probe demonstrated that the strains isolated in 1987 showed an identical cholera toxin genotype, whereas O1 strains isolated in 1994 and 1995 showed the same genotype that was distinct from the genotype of strains isolated in 1987. These results were supported by the results of antibiotic susceptibility testing, in which strains isolated in 1987 showed resistance to polymyxin B only, while each of the strains from 1994 and 1995 showed resistance to polymyxin B, trimethoprim-sulfamethoxazole, and the vibriostatic agent O/129. Although our results are based on a limited number of V. cholerae O1 strains, they suggest that the epidemic in Guinea-Bissau in 1994 and 1995 was due to the introduction of a new strain to the country.

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Selected References

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  1. Bagchi K., Echeverria P., Arthur J. D., Sethabutr O., Serichantalergs O., Hoge C. W. Epidemic of diarrhea caused by Vibrio cholerae non-O1 that produced heat-stable toxin among Khmers in a camp in Thailand. J Clin Microbiol. 1993 May;31(5):1315–1317. doi: 10.1128/jcm.31.5.1315-1317.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bhadra R. K., Choudhury S. R., Das J. Vibrio cholerae O139 El Tor biotype. Lancet. 1994 Mar 19;343(8899):728–728. doi: 10.1016/s0140-6736(94)91604-7. [DOI] [PubMed] [Google Scholar]
  3. Cameron D. N., Khambaty F. M., Wachsmuth I. K., Tauxe R. V., Barrett T. J. Molecular characterization of Vibrio cholerae O1 strains by pulsed-field gel electrophoresis. J Clin Microbiol. 1994 Jul;32(7):1685–1690. doi: 10.1128/jcm.32.7.1685-1690.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coelho A., Andrade J. R., Vicente A. C., Salles C. A. New variant of Vibrio cholerae O1 from clinical isolates in Amazonia. J Clin Microbiol. 1995 Jan;33(1):114–118. doi: 10.1128/jcm.33.1.114-118.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dalsgaard A., Albert M. J., Taylor D. N., Shimada T., Meza R., Serichantalergs O., Echeverria P. Characterization of Vibrio cgolerae non-O1 serogroups obtained from an outbreak of diarrhea in Lima, Peru. J Clin Microbiol. 1995 Oct;33(10):2715–2722. doi: 10.1128/jcm.33.10.2715-2722.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dalsgaard A., Echeverria P., Larsen J. L., Siebeling R., Serichantalergs O., Huss H. H. Application of ribotyping for differentiating Vibrio cholerae non-O1 isolated from shrimp farms in Thailand. Appl Environ Microbiol. 1995 Jan;61(1):245–251. doi: 10.1128/aem.61.1.245-251.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dalsgaard A., Serichantalergs O., Pitarangsi C., Echeverria P. Molecular characterization and antibiotic susceptibility of Vibrio cholerae non-O1. Epidemiol Infect. 1995 Feb;114(1):51–63. doi: 10.1017/s0950268800051906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Echeverria P., Hoge C. W., Bodhidatta L., Serichantalergs O., Dalsgaard A., Eampokalap B., Perrault J., Pazzaglia G., O'Hanley P., English C. Molecular characterization of Vibrio cholerae O139 isolates from Asia. Am J Trop Med Hyg. 1995 Feb;52(2):124–127. doi: 10.4269/ajtmh.1995.52.124. [DOI] [PubMed] [Google Scholar]
  9. Glass R. I., Claeson M., Blake P. A., Waldman R. J., Pierce N. F. Cholera in Africa: lessons on transmission and control for Latin America. Lancet. 1991 Sep 28;338(8770):791–795. doi: 10.1016/0140-6736(91)90673-d. [DOI] [PubMed] [Google Scholar]
  10. Hanchalay S., Seriwatana J., Echeverria P., Holmgren J., Tirapat C., Moseley S. L., Taylor D. N. Non-O1 Vibrio cholerae in Thailand: homology with cloned cholera toxin genes. J Clin Microbiol. 1985 Feb;21(2):288–289. doi: 10.1128/jcm.21.2.288-289.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huq A., Alam M., Parveen S., Colwell R. R. Occurrence of resistance to vibriostatic compound 0/129 in Vibrio cholerae 01 isolated from clinical and environmental samples in Bangladesh. J Clin Microbiol. 1992 Jan;30(1):219–221. doi: 10.1128/jcm.30.1.219-221.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Islam M. S., Drasar B. S., Sack R. B. Probable role of blue-green algae in maintaining endemicity and seasonality of cholera in Bangladesh: a hypothesis. J Diarrhoeal Dis Res. 1994 Dec;12(4):245–256. [PubMed] [Google Scholar]
  13. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Macrina F. L., Kopecko D. J., Jones K. R., Ayers D. J., McCowen S. M. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid. 1978 Jun;1(3):417–420. doi: 10.1016/0147-619x(78)90056-2. [DOI] [PubMed] [Google Scholar]
  15. Mekalanos J. J. Duplication and amplification of toxin genes in Vibrio cholerae. Cell. 1983 Nov;35(1):253–263. doi: 10.1016/0092-8674(83)90228-3. [DOI] [PubMed] [Google Scholar]
  16. Murray M. G., Thompson W. F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980 Oct 10;8(19):4321–4325. doi: 10.1093/nar/8.19.4321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mølbak K., Wested N., Højlyng N., Scheutz F., Gottschau A., Aaby P., da Silva A. P. The etiology of early childhood diarrhea: a community study from Guinea-Bissau. J Infect Dis. 1994 Mar;169(3):581–587. doi: 10.1093/infdis/169.3.581. [DOI] [PubMed] [Google Scholar]
  18. Olsen J. E., Brown D. J., Baggesen D. L., Bisgaard M. Biochemical and molecular characterization of Salmonella enterica serovar berta, and comparison of methods for typing. Epidemiol Infect. 1992 Apr;108(2):243–260. doi: 10.1017/s0950268800049724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Olsen J. E., Larsen J. L. Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid. Appl Environ Microbiol. 1990 Oct;56(10):3130–3132. doi: 10.1128/aem.56.10.3130-3132.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Popovic T., Bopp C., Olsvik O., Wachsmuth K. Epidemiologic application of a standardized ribotype scheme for Vibrio cholerae O1. J Clin Microbiol. 1993 Sep;31(9):2474–2482. doi: 10.1128/jcm.31.9.2474-2482.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rochelle P. A., Fry J. C., Day M. J., Bale M. J. An accurate method for estimating sizes of small and large plasmids and DNA fragments by gel electrophoresis. J Gen Microbiol. 1986 Jan;132(1):53–59. doi: 10.1099/00221287-132-1-53. [DOI] [PubMed] [Google Scholar]
  22. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  23. Tabtieng R., Wattanasri S., Echeverria P., Seriwatana J., Bodhidatta L., Chatkaeomorakot A., Rowe B. An epidemic of Vibrio cholerae el tor Inaba resistant to several antibiotics with a conjugative group C plasmid coding for type II dihydrofolate reductase in Thailand. Am J Trop Med Hyg. 1989 Dec;41(6):680–686. doi: 10.4269/ajtmh.1989.41.680. [DOI] [PubMed] [Google Scholar]
  24. Threlfall E. J., Rowe B., Ferguson J. L., Ward L. R. Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J Hyg (Lond) 1986 Dec;97(3):419–426. doi: 10.1017/s0022172400063609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wright A. C., Guo Y., Johnson J. A., Nataro J. P., Morris J. G., Jr Development and testing of a nonradioactive DNA oligonucleotide probe that is specific for Vibrio cholerae cholera toxin. J Clin Microbiol. 1992 Sep;30(9):2302–2306. doi: 10.1128/jcm.30.9.2302-2306.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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