Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1996 May;34(5):1224–1227. doi: 10.1128/jcm.34.5.1224-1227.1996

Detection of Yersinia enterocolitica serogroup O:3 by a PCR method.

V Weynants 1, V Jadot 1, P A Denoel 1, A Tibor 1, J J Letesson 1
PMCID: PMC228986  PMID: 8727907

Abstract

Yersinia enterocolitica is the etiologic agent of a range of clinical situations in humans, but only a small number of serotypes are involved. Among these, Y. enterocolitica O:3 is the most frequently implicated. A PCR method was developed to detect Y. enterocolitica O:3. For this purpose, two pairs of primers were designed to amplify two fragments of the rfb cluster of Y. enterocolitica O:3: a 253-bp fragment of the rfbB gene and a 405-bp fragment of the rfbC gene. A specific detection was obtained only with rfbC primers, which yielded a PCR product of the expected size exclusively with pathogenic Y. enterocolitica of serotype O:3. This pair of primers was combined with the ail, inv, and virF primers previously described (H. Nakajima, M. Inoue, T. Mori, K.-I. Itoh, E. Arakawa, and H. Watanabe, J. Clin. Microbiol. 30:2484-2486, 1992) to allow both the detection and the differentiation between Y. pseudotuberculosis, pathogenic Y. enterocolitica of serotype O:3 and other pathogenic Y. enterocolitica.

Full Text

The Full Text of this article is available as a PDF (405.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bottone E. J., Gullans C. R., Sierra M. F. Disease spectrum of Yersinia enterocolitica serogroup 0:3, the predominant cause of human infection in New York City. Contrib Microbiol Immunol. 1987;9:56–60. [PubMed] [Google Scholar]
  2. Collins D. M., Stephens D. M., de Lisle G. W. Comparison of polymerase chain reaction tests and faecal culture for detecting Mycobacterium paratuberculosis in bovine faeces. Vet Microbiol. 1993 Sep;36(3-4):289–299. doi: 10.1016/0378-1135(93)90095-o. [DOI] [PubMed] [Google Scholar]
  3. Cornelis G. R., Biot T., Lambert de Rouvroit C., Michiels T., Mulder B., Sluiters C., Sory M. P., Van Bouchaute M., Vanooteghem J. C. The Yersinia yop regulon. Mol Microbiol. 1989 Oct;3(10):1455–1459. doi: 10.1111/j.1365-2958.1989.tb00129.x. [DOI] [PubMed] [Google Scholar]
  4. Fenwick S. G., Murray A. Detection of pathogenic Yersinia enterocolitica by polymerase chain reaction. Lancet. 1991 Feb 23;337(8739):496–497. doi: 10.1016/0140-6736(91)93436-d. [DOI] [PubMed] [Google Scholar]
  5. Fluit A. C., Torensma R., Visser M. J., Aarsman C. J., Poppelier M. J., Keller B. H., Klapwijk P., Verhoef J. Detection of Listeria monocytogenes in cheese with the magnetic immuno-polymerase chain reaction assay. Appl Environ Microbiol. 1993 May;59(5):1289–1293. doi: 10.1128/aem.59.5.1289-1293.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gumerlock P. H., Tang Y. J., Meyers F. J., Silva J., Jr Use of the polymerase chain reaction for the specific and direct detection of Clostridium difficile in human feces. Rev Infect Dis. 1991 Nov-Dec;13(6):1053–1060. doi: 10.1093/clinids/13.6.1053. [DOI] [PubMed] [Google Scholar]
  7. Hartung M., Gerigk K. Yersinia in effluents from the food-processing industry. Rev Sci Tech. 1991 Sep;10(3):799–811. doi: 10.20506/rst.10.3.566. [DOI] [PubMed] [Google Scholar]
  8. Kapperud G., Vardund T., Skjerve E., Hornes E., Michaelsen T. E. Detection of pathogenic Yersinia enterocolitica in foods and water by immunomagnetic separation, nested polymerase chain reactions, and colorimetric detection of amplified DNA. Appl Environ Microbiol. 1993 Sep;59(9):2938–2944. doi: 10.1128/aem.59.9.2938-2944.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kapperud G. Yersinia enterocolitica in food hygiene. Int J Food Microbiol. 1991 Jan;12(1):53–65. doi: 10.1016/0168-1605(91)90047-s. [DOI] [PubMed] [Google Scholar]
  10. Li Q., DiSanto M. E., Magee W. E. A rapid method for detection of Yersinia enterocolitica serotype O:3 in pig feces using monoclonal antibodies. Vet Microbiol. 1992 Jul;32(1):29–41. doi: 10.1016/0378-1135(92)90004-d. [DOI] [PubMed] [Google Scholar]
  11. Nakajima H., Inoue M., Mori T., Itoh K., Arakawa E., Watanabe H. Detection and identification of Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica by an improved polymerase chain reaction method. J Clin Microbiol. 1992 Sep;30(9):2484–2486. doi: 10.1128/jcm.30.9.2484-2486.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Skurnik M., Toivanen P. Yersinia enterocolitica lipopolysaccharide: genetics and virulence. Trends Microbiol. 1993 Jul;1(4):148–152. doi: 10.1016/0966-842x(93)90130-j. [DOI] [PubMed] [Google Scholar]
  13. Tauxe R. V., Vandepitte J., Wauters G., Martin S. M., Goossens V., De Mol P., Van Noyen R., Thiers G. Yersinia enterocolitica infections and pork: the missing link. Lancet. 1987 May 16;1(8542):1129–1132. doi: 10.1016/s0140-6736(87)91683-7. [DOI] [PubMed] [Google Scholar]
  14. Wauters G., Aleksić S., Charlier J., Schulze G. Somatic and flagellar antigens of Yersinia enterocolitica and related species. Contrib Microbiol Immunol. 1991;12:239–243. [PubMed] [Google Scholar]
  15. Widjojoatmodjo M. N., Fluit A. C., Torensma R., Verdonk G. P., Verhoef J. The magnetic immuno polymerase chain reaction assay for direct detection of salmonellae in fecal samples. J Clin Microbiol. 1992 Dec;30(12):3195–3199. doi: 10.1128/jcm.30.12.3195-3199.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wren B. W., Tabaqchali S. Detection of pathogenic Yersinia enterocolitica by the polymerase chain reaction. Lancet. 1990 Sep 15;336(8716):693–693. doi: 10.1016/0140-6736(90)92191-j. [DOI] [PubMed] [Google Scholar]
  17. Zhang L., al-Hendy A., Toivanen P., Skurnik M. Genetic organization and sequence of the rfb gene cluster of Yersinia enterocolitica serotype O:3: similarities to the dTDP-L-rhamnose biosynthesis pathway of Salmonella and to the bacterial polysaccharide transport systems. Mol Microbiol. 1993 Jul;9(2):309–321. doi: 10.1111/j.1365-2958.1993.tb01692.x. [DOI] [PubMed] [Google Scholar]
  18. al-Hendy A., Toivanen P., Skurnik M. Expression cloning of Yersinia enterocolitica O:3 rfb gene cluster in Escherichia coli K12. Microb Pathog. 1991 Jan;10(1):47–59. doi: 10.1016/0882-4010(91)90065-i. [DOI] [PubMed] [Google Scholar]
  19. al-Hendy A., Toivanen P., Skurnik M. Lipopolysaccharide O side chain of Yersinia enterocolitica O:3 is an essential virulence factor in an orally infected murine model. Infect Immun. 1992 Mar;60(3):870–875. doi: 10.1128/iai.60.3.870-875.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES