Abstract
Agglutination reactions obtained with three commercial latex reagents for detecting Escherichia coli O157 antigen (Oxoid Diagnostic Reagents, Hampshire, England; Pro-Lab Inc., Richmond Hill, Ontario, Canada; and Remel Microbiology Products, Lenexa, Kans.) and one for detecting H7 antigen (Remel) were compared with those obtained by standard serologic methods by using the Centers for Disease Control and Prevention (CDC) reference antisera for O157 and H7 antigens. For 159 strains of E. coli and related organisms, the Oxoid, Pro-Lab, and Remel O157 latex reagents each had a sensitivity and specificity of 100% compared with the CDC reference antiserum. For 106 strains of E. coli and related organisms that were not enhanced for motility through semisolid medium, the Remel H7 latex reagent had a sensitivity of 96% and a specificity of 100% compared with the standard tube agglutination method with CDC H7 antiserum. Measures to enhance motility were needed for some strains to detect the H7 antigen. Our findings demonstrate that the commercial latex reagents are good alternatives to standard serologic methods for identifying the O157 and H7 antigens of E. coli.
Full Text
The Full Text of this article is available as a PDF (166.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bettelheim K. A., Evangelidis H., Pearce J. L., Sowers E., Strockbine N. A. Isolation of a Citrobacter freundii strain which carries the Escherichia coli O157 antigen. J Clin Microbiol. 1993 Mar;31(3):760–761. doi: 10.1128/jcm.31.3.760-761.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Griffin P. M., Ostroff S. M., Tauxe R. V., Greene K. D., Wells J. G., Lewis J. H., Blake P. A. Illnesses associated with Escherichia coli O157:H7 infections. A broad clinical spectrum. Ann Intern Med. 1988 Nov 1;109(9):705–712. doi: 10.7326/0003-4819-109-9-705. [DOI] [PubMed] [Google Scholar]
- Karmali M. A., Petric M., Lim C., Fleming P. C., Arbus G. S., Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis. 1985 May;151(5):775–782. doi: 10.1093/infdis/151.5.775. [DOI] [PubMed] [Google Scholar]
- Lior H., Borczyk A. A. False positive identifications of Escherichia coli O157. Lancet. 1987 Feb 7;1(8528):333–333. doi: 10.1016/s0140-6736(87)92065-4. [DOI] [PubMed] [Google Scholar]
- March S. B., Ratnam S. Latex agglutination test for detection of Escherichia coli serotype O157. J Clin Microbiol. 1989 Jul;27(7):1675–1677. doi: 10.1128/jcm.27.7.1675-1677.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- March S. B., Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol. 1986 May;23(5):869–872. doi: 10.1128/jcm.23.5.869-872.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pai C. H., Gordon R., Sims H. V., Bryan L. E. Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Clinical, epidemiologic, and bacteriologic features. Ann Intern Med. 1984 Dec;101(6):738–742. doi: 10.7326/0003-4819-101-6-738. [DOI] [PubMed] [Google Scholar]
- Perry M. B., Bundle D. R. Antigenic relationships of the lipopolysaccharides of Escherichia hermannii strains with those of Escherichia coli O157:H7, Brucella melitensis, and Brucella abortus. Infect Immun. 1990 May;58(5):1391–1395. doi: 10.1128/iai.58.5.1391-1395.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rice E. W., Sowers E. G., Johnson C. H., Dunnigan M. E., Strockbine N. A., Edberg S. C. Serological cross-reactions between Escherichia coli O157 and other species of the genus Escherichia. J Clin Microbiol. 1992 May;30(5):1315–1316. doi: 10.1128/jcm.30.5.1315-1316.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riley L. W., Remis R. S., Helgerson S. D., McGee H. B., Wells J. G., Davis B. R., Hebert R. J., Olcott E. S., Johnson L. M., Hargrett N. T. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983 Mar 24;308(12):681–685. doi: 10.1056/NEJM198303243081203. [DOI] [PubMed] [Google Scholar]