Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1996 May;34(5):1293–1295. doi: 10.1128/jcm.34.5.1293-1295.1996

Restriction fragment length polymorphism of the tdh and trh genes in clinical Vibrio parahaemolyticus strains.

O Suthienkul 1, T Iida 1, K S Park 1, M Ishibashi 1, S Supavej 1, K Yamamoto 1, T Honda 1
PMCID: PMC229002  PMID: 8727923

Abstract

The restriction fragment length polymorphism of the genes encoding thermostable direct hemolysin (tdh) and thermostable direct hemolysin-related hemolysin (trh) was analyzed for 137 strains of Vibrio parahaemolyticus isolated from specimens from diarrheal patients in Thailand. The HindIII restriction fragment patterns of tdh and trh were grouped into five and four types, respectively. A strong association between the restriction fragment patterns of tdh and trh was observed with V. parahaemolyticus strains.

Full Text

The Full Text of this article is available as a PDF (265.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Honda S., Matsumoto S., Miwatani T., Honda T. A survey of urease-positive Vibrio parahaemolyticus strains isolated from traveller's diarrhea, sea water and imported frozen sea foods. Eur J Epidemiol. 1992 Nov;8(6):861–864. doi: 10.1007/BF00145333. [DOI] [PubMed] [Google Scholar]
  2. Honda T., Abad-Lapuebla M. A., Ni Y. X., Yamamoto K., Miwatani T. Characterization of a new thermostable direct haemolysin produced by a Kanagawa-phenomenon-negative clinical isolate of Vibrio parahaemolyticus. J Gen Microbiol. 1991 Feb;137(2):253–259. doi: 10.1099/00221287-137-2-253. [DOI] [PubMed] [Google Scholar]
  3. Honda T., Ni Y. X., Miwatani T. Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin. Infect Immun. 1988 Apr;56(4):961–965. doi: 10.1128/iai.56.4.961-965.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hondo S., Goto I., Minematsu I., Ikeda N., Asano N., Ishibashi M., Kinoshita Y., Nishibuchi N., Honda T., Miwatani T. Gastroenteritis due to Kanagawa negative Vibrio parahaemolyticus. Lancet. 1987 Feb 7;1(8528):331–332. doi: 10.1016/s0140-6736(87)92062-9. [DOI] [PubMed] [Google Scholar]
  5. Kishishita M., Matsuoka N., Kumagai K., Yamasaki S., Takeda Y., Nishibuchi M. Sequence variation in the thermostable direct hemolysin-related hemolysin (trh) gene of Vibrio parahaemolyticus. Appl Environ Microbiol. 1992 Aug;58(8):2449–2457. doi: 10.1128/aem.58.8.2449-2457.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nishibuchi M., Kaper J. B. Thermostable direct hemolysin gene of Vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect Immun. 1995 Jun;63(6):2093–2099. doi: 10.1128/iai.63.6.2093-2099.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nishibuchi M., Taniguchi T., Misawa T., Khaeomanee-Iam V., Honda T., Miwatani T. Cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahaemolyticus. Infect Immun. 1989 Sep;57(9):2691–2697. doi: 10.1128/iai.57.9.2691-2697.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sakazaki R., Tamura K., Kato T., Obara Y., Yamai S. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. 3. Enteropathogenicity. Jpn J Med Sci Biol. 1968 Oct;21(5):325–331. doi: 10.7883/yoken1952.21.325. [DOI] [PubMed] [Google Scholar]
  9. Shirai H., Ito H., Hirayama T., Nakamoto Y., Nakabayashi N., Kumagai K., Takeda Y., Nishibuchi M. Molecular epidemiologic evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis. Infect Immun. 1990 Nov;58(11):3568–3573. doi: 10.1128/iai.58.11.3568-3573.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Suthienkul O., Ishibashi M., Iida T., Nettip N., Supavej S., Eampokalap B., Makino M., Honda T. Urease production correlates with possession of the trh gene in Vibrio parahaemolyticus strains isolated in Thailand. J Infect Dis. 1995 Nov;172(5):1405–1408. doi: 10.1093/infdis/172.5.1405. [DOI] [PubMed] [Google Scholar]
  11. Suzuki N., Ueda Y., Mori H., Miyagi K., Noda K., Hirose H., Oosumi Y., Ishibashi M., Yoh M., Yamamoto K. [Serotypes of urease producing Vibrio parahaemolyticus and their relation to possession of tdh and trh genes]. Kansenshogaku Zasshi. 1994 Sep;68(9):1068–1074. doi: 10.11150/kansenshogakuzasshi1970.68.1068. [DOI] [PubMed] [Google Scholar]
  12. Terai A., Baba K., Shirai H., Yoshida O., Takeda Y., Nishibuchi M. Evidence for insertion sequence-mediated spread of the thermostable direct hemolysin gene among Vibrio species. J Bacteriol. 1991 Aug;173(16):5036–5046. doi: 10.1128/jb.173.16.5036-5046.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yamamoto K., Honda T., Miwatani T., Tamatsukuri S., Shibata S. Enzyme-labeled oligonucleotide probes for detection of the genes for thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) of Vibrio parahaemolyticus. Can J Microbiol. 1992 May;38(5):410–416. doi: 10.1139/m92-069. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES