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. 1996 May;34(5):1299–1302. doi: 10.1128/jcm.34.5.1299-1302.1996

Polymorphism in Brucella strains detected by studying distribution of two short repetitive DNA elements.

E Mercier 1, E Jumas-Bilak 1, A Allardet-Servent 1, D O'Callaghan 1, M Ramuz 1
PMCID: PMC229004  PMID: 8727925

Abstract

Thirty-four Brucella reference or field strains representing all the species and biovars were studied by repetitive element sequence-based PCR, a PCR using primers complementary to two enterobacterial short repetitive sequences: repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequences. All the stains showed a positive amplification, suggesting that the Brucella genome contains such sequences. Repetitive extragenic palindromic PCR was less discriminating than enterobacterial repetitive intergenic consensus PCR in terms of distinguishing strains, but a combination of the two methods was able to distinguish all the isolates, except for some strains belonging to biovars 3 and 9 of Brucella abortus. Repetitive element sequence-based PCR appears to be a simple and useful method for the study of brucellosis epidemiology.

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Selected References

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