Abstract
Twenty-nine Mycobacterium reference strains representing 10 species and 60 mycobacterial cultures isolated from sputum specimens were studied. These cultures were grown in Bactec 7H12B medium (Becton Dickinson and Co., Paramus, N.J.) supplemented with oleic acid-albumin-dextrose-catalase enrichment broth (Becton Dickinson and Co., Cockeysville, Md.). The cultures were analyzed by gas-liquid chromatography for their fatty acids, secondary alcohols, and mycolic acid cleavage products. All of the clinical isolates could be identified by comparing their gas-liquid chromatography profiles with those of the reference strains. The data indicate that this method significantly shortens the turnaround time and could be used for the early detection and identification of mycobacterial species.
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Selected References
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- Butler W. R., Warren N. G., Kubica G. P., Kilburn J. O. Modified method for testing the quality of albumin-containing enrichments used in growth media for mycobacteria. J Clin Microbiol. 1990 May;28(5):1068–1070. doi: 10.1128/jcm.28.5.1068-1070.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cage G. D. Direct identification of Mycobacterium species in BACTEC 7H12B medium by high-performance liquid chromatography. J Clin Microbiol. 1994 Feb;32(2):521–524. doi: 10.1128/jcm.32.2.521-524.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chou S., Aldova E., Kasatiya S. Cellular fatty acid composition of Plesiomonas shigelloides. J Clin Microbiol. 1991 May;29(5):1072–1074. doi: 10.1128/jcm.29.5.1072-1074.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guerrant G. O., Lambert M. A., Moss C. W. Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria. J Clin Microbiol. 1981 May;13(5):899–907. doi: 10.1128/jcm.13.5.899-907.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huebner R. E., Good R. C., Tokars J. I. Current practices in mycobacteriology: results of a survey of state public health laboratories. J Clin Microbiol. 1993 Apr;31(4):771–775. doi: 10.1128/jcm.31.4.771-775.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jantzen E., Tangen T., Eng J. Gas chromatography of mycobacterial fatty acids and alcohols: diagnostic applications. APMIS. 1989 Nov;97(11):1037–1045. doi: 10.1111/j.1699-0463.1989.tb00515.x. [DOI] [PubMed] [Google Scholar]
- Lambert M. A., Moss C. W., Silcox V. A., Good R. C. Analysis of mycolic acid cleavage products and cellular fatty acids of Mycobacterium species by capillary gas chromatography. J Clin Microbiol. 1986 Apr;23(4):731–736. doi: 10.1128/jcm.23.4.731-736.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luquin M., Ausina V., López Calahorra F., Belda F., García Barceló M., Celma C., Prats G. Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria. J Clin Microbiol. 1991 Jan;29(1):120–130. doi: 10.1128/jcm.29.1.120-130.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Minnikin D. E., Dobson G., Goodfellow M., Draper P., Magnusson M. Quantitative comparison of the mycolic and fatty acid compositions of Mycobacterium leprae and Mycobacterium gordonae. J Gen Microbiol. 1985 Aug;131(8):2013–2021. doi: 10.1099/00221287-131-8-2013. [DOI] [PubMed] [Google Scholar]
- Parez J. J., Fauville-Dufaux M., Dossogne J. L., de Hoffmann E., Pouthier F. Faster identification of mycobacteria using gas liquid and thin layer chromatography. Eur J Clin Microbiol Infect Dis. 1994 Sep;13(9):717–725. doi: 10.1007/BF02276054. [DOI] [PubMed] [Google Scholar]
- Tisdall P. A., Roberts G. D., Anhalt J. P. Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone. J Clin Microbiol. 1979 Oct;10(4):506–514. doi: 10.1128/jcm.10.4.506-514.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Valero-Guillén P. L., Pacheco F., Martín-Luengo F. Fatty acid composition and mycolic acid pattern of some chromogenic mycobacteria. J Appl Bacteriol. 1985 Aug;59(2):113–126. doi: 10.1111/j.1365-2672.1985.tb03309.x. [DOI] [PubMed] [Google Scholar]