Abstract
We evaluated two immunochromatographic assays (ICAs), Dainascreen HBsAg for detecting human hepatitis hepatitis B surface antigen (HBsAg) and Dainascreen Ausab for detecting human hepatitis B surface antibody (anti-HBs) in human serum. The ICA systems are composed of a comb-shaped device that contains nitrocellulose strips on which complexes of HBsAg and anti-HBs can be visualized. The results can be read within 15 min of incubation. The limit of detection for HBsAg was 3.1 ng/ml and that for anti-HBs was 42 mIU/ml. Results of HBsAg detection agreed completely with those of conventional enzyme immunoassays (EIAs) and showed a 100% sensitivity (158 of 158 samples) and a 100% specificity (304 of 304 samples). The Dainascreen Ausab detected 184 of the 199 EIA-positive samples (sensitivity, 92.5%) and yielded 6 positive results among the 281 EIA-negative samples (specificity, 97.9%). The ICA systems are rapid and sensitive methods for detecting HBsAg and anti-HBs. They are low-cost systems that need no complex instrumentation for analysis and can be recommended for routine use in clinical microbiology laboratories.
Full Text
The Full Text of this article is available as a PDF (298.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barr A., Dow B. C., Macvarish I. HBsAg detection--results of comparative large scale testing of blood donations. Med Lab Sci. 1979 Apr;36(2):109–114. [PubMed] [Google Scholar]
- Hendry R. M., Herzog L. W., Herrman J. E., Meehan R. E. Comparison of enzyme immunoassay with radioimmunoassay for the detection of hepatitis B surface antigen. J Med Microbiol. 1983 May;16(2):111–116. doi: 10.1099/00222615-16-2-111. [DOI] [PubMed] [Google Scholar]
- Kroes A. C., Quint W. G., Heijtink R. A. Significance of isolated hepatitis B core antibodies detected by enzyme immunoassay in a high risk population. J Med Virol. 1991 Oct;35(2):96–100. doi: 10.1002/jmv.1890350205. [DOI] [PubMed] [Google Scholar]
- McCready J. A., Morens D., Fields H. A., Coleman P. J., Kane M., Schatz G. Evaluation of enzyme immunoassay (EIA) as a screening method for hepatitis B markers in an open population. Epidemiol Infect. 1991 Dec;107(3):673–684. doi: 10.1017/s0950268800049372. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McMahon B. J., Parkinson A. J., Helminiak C., Wainwright R. B., Bulkow L., Kellerman-Douglas A., Schoenberg S., Ritter D. Response to hepatitis B vaccine of persons positive for antibody to hepatitis B core antigen. Gastroenterology. 1992 Aug;103(2):590–594. doi: 10.1016/0016-5085(92)90851-o. [DOI] [PubMed] [Google Scholar]
- Pár A., Luminita S., Erdósy I., Doru D., Kádas I., Paál M., Brasch G., Beró T., Jávor T. Hepatitis vírus (HBV, HCV, HDV) markerek idült májbetegségekben. Osszehasonlító vizsgálatok két közép-kelet-európai országban. Orv Hetil. 1992 Jul 5;133 (Suppl 1):48–50. [PubMed] [Google Scholar]
- Ranga S., Pandey J., Talib V. H. Evaluation of two techniques (RPHA and latex agglutination) for use as a screening tool for HBsAg. Indian J Pathol Microbiol. 1994 Oct;37(4):375–380. [PubMed] [Google Scholar]
- Shiels M. T., Taswell H. F., Czaja A. J., Nelson C., Swenke P. Frequency and significance of concurrent hepatitis B surface antigen and antibody in acute and chronic hepatitis B. Gastroenterology. 1987 Oct;93(4):675–680. doi: 10.1016/0016-5085(87)90427-6. [DOI] [PubMed] [Google Scholar]
- Treichel U., Meyer zum Büschenfelde K. H., Stockert R. J., Poralla T., Gerken G. The asialoglycoprotein receptor mediates hepatic binding and uptake of natural hepatitis B virus particles derived from viraemic carriers. J Gen Virol. 1994 Nov;75(Pt 11):3021–3029. doi: 10.1099/0022-1317-75-11-3021. [DOI] [PubMed] [Google Scholar]
- Van Damme P., Cramm M., Van der Auwera J. C., Vranckx R., Meheus A. Horizontal transmission of hepatitis B virus. Lancet. 1995 Jan 7;345(8941):27–29. doi: 10.1016/s0140-6736(95)91155-3. [DOI] [PubMed] [Google Scholar]