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. 1996 Jun;34(6):1465–1467. doi: 10.1128/jcm.34.6.1465-1467.1996

Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay.

S L Klespies 1, D E Cebula 1, C L Kelley 1, D Galehouse 1, C C Maurer 1
PMCID: PMC229043  PMID: 8735099

Abstract

Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interval. The data suggest that a spin-amplified shell vial indirect immunofluorescence assay may be useful for the detection of enterovirus from clinical specimens.

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Selected References

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