Abstract
The porA genes from serogroup A, subgroup III strains isolated in the People's Republic of China in 1966 and in 1984 and 1985 were amplified by PCR at an annealing temperature of 75 degrees C. The DNA sequences (5 strains) and the restriction patterns generated by MspI (14 strains) were identical, unlike the results reported by Peixuan et al. (Z. Peixuan, H. Xujing, and X. Li, J. Clin. Microbiol. 33:458-462, 1995). Furthermore, PCR products which were amplified at an annealing temperature of 60 degrees C, as described by Peixuan et al., were heterogeneous in our study.
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Selected References
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- Achtman M. Clonal spread of serogroup A meningococci: a paradigm for the analysis of microevolution in bacteria. Mol Microbiol. 1994 Jan;11(1):15–22. doi: 10.1111/j.1365-2958.1994.tb00285.x. [DOI] [PubMed] [Google Scholar]
- Achtman M., Kusecek B., Morelli G., Eickmann K., Wang J. F., Crowe B., Wall R. A., Hassan-King M., Moore P. S., Zollinger W. A comparison of the variable antigens expressed by clone IV-1 and subgroup III of Neisseria meningitidis serogroup A. J Infect Dis. 1992 Jan;165(1):53–68. doi: 10.1093/infdis/165.1.53. [DOI] [PubMed] [Google Scholar]
- Bassam B. J., Caetano-Anollés G., Gresshoff P. M. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem. 1991 Jul;196(1):80–83. doi: 10.1016/0003-2697(91)90120-i. [DOI] [PubMed] [Google Scholar]
- Hobbs M. M., Seiler A., Achtman M., Cannon J. G. Microevolution within a clonal population of pathogenic bacteria: recombination, gene duplication and horizontal genetic exchange in the opa gene family of Neisseria meningitidis. Mol Microbiol. 1994 Apr;12(2):171–180. doi: 10.1111/j.1365-2958.1994.tb01006.x. [DOI] [PubMed] [Google Scholar]
- Lomholt H., Poulsen K., Kilian M. Comparative characterization of the iga gene encoding IgA1 protease in Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae. Mol Microbiol. 1995 Feb;15(3):495–506. doi: 10.1111/j.1365-2958.1995.tb02263.x. [DOI] [PubMed] [Google Scholar]
- Maiden M. C., Suker J., McKenna A. J., Bygraves J. A., Feavers I. M. Comparison of the class 1 outer membrane proteins of eight serological reference strains of Neisseria meningitidis. Mol Microbiol. 1991 Mar;5(3):727–736. doi: 10.1111/j.1365-2958.1991.tb00743.x. [DOI] [PubMed] [Google Scholar]
- Moore P. S., Reeves M. W., Schwartz B., Gellin B. G., Broome C. V. Intercontinental spread of an epidemic group A Neisseria meningitidis strain. Lancet. 1989 Jul 29;2(8657):260–263. doi: 10.1016/s0140-6736(89)90439-x. [DOI] [PubMed] [Google Scholar]
- Morelli G., del Valle J., Lammel C. J., Pohlner J., Müller K., Blake M., Brooks G. F., Meyer T. F., Koumaré B., Brieske N. Immunogenicity and evolutionary variability of epitopes within IgA1 protease from serogroup A Neisseria meningitidis. Mol Microbiol. 1994 Jan;11(1):175–187. doi: 10.1111/j.1365-2958.1994.tb00299.x. [DOI] [PubMed] [Google Scholar]
- Olyhoek T., Crowe B. A., Achtman M. Clonal population structure of Neisseria meningitidis serogroup A isolated from epidemics and pandemics between 1915 and 1983. Rev Infect Dis. 1987 Jul-Aug;9(4):665–692. doi: 10.1093/clinids/9.4.665. [DOI] [PubMed] [Google Scholar]
- Suker J., Feavers I. M., Achtman M., Morelli G., Wang J. F., Maiden M. C. The porA gene in serogroup A meningococci: evolutionary stability and mechanism of genetic variation. Mol Microbiol. 1994 Apr;12(2):253–265. doi: 10.1111/j.1365-2958.1994.tb01014.x. [DOI] [PubMed] [Google Scholar]
- Wang J. F., Caugant D. A., Li X., Hu X., Poolman J. T., Crowe B. A., Achtman M. Clonal and antigenic analysis of serogroup A Neisseria meningitidis with particular reference to epidemiological features of epidemic meningitis in the People's Republic of China. Infect Immun. 1992 Dec;60(12):5267–5282. doi: 10.1128/iai.60.12.5267-5282.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zhu P., Hu X., Xu L. Typing Neisseria meningitidis by analysis of restriction fragment length polymorphisms in the gene encoding the class 1 outer membrane protein: application to assessment of epidemics throughout the last 4 decades in China. J Clin Microbiol. 1995 Feb;33(2):458–462. doi: 10.1128/jcm.33.2.458-462.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]