Abstract
Extended incubation of culture plates was studied to see if the recovery of Bordetella spp. from nasopharyngeal swabs could be improved. Forty-eight Bordetella isolates were recovered from 103 children (overall positive-culture rate, 46.6%) who met the clinical case definition of pertussis. Seven of 44 (16%) B. pertussis isolates and 2 of 4 (50%) B. parapertussis isolates were recovered only after extended incubation of nasopharyngeal cultures up to 12 days.
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- Conway S. P., Balfour A. H., Ross H. Serologic diagnosis of whooping cough by enzyme-linked immunosorbent assay. Pediatr Infect Dis J. 1988 Aug;7(8):570–574. [PubMed] [Google Scholar]
- Friedman R. L. Pertussis: the disease and new diagnostic methods. Clin Microbiol Rev. 1988 Oct;1(4):365–376. doi: 10.1128/cmr.1.4.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glare E. M., Paton J. C., Premier R. R., Lawrence A. J., Nisbet I. T. Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction. J Clin Microbiol. 1990 Sep;28(9):1982–1987. doi: 10.1128/jcm.28.9.1982-1987.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halperin S. A., Bortolussi R., Wort A. J. Evaluation of culture, immunofluorescence, and serology for the diagnosis of pertussis. J Clin Microbiol. 1989 Apr;27(4):752–757. doi: 10.1128/jcm.27.4.752-757.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- He Q., Mertsola J., Soini H., Viljanen M. K. Sensitive and specific polymerase chain reaction assays for detection of Bordetella pertussis in nasopharyngeal specimens. J Pediatr. 1994 Mar;124(3):421–426. doi: 10.1016/s0022-3476(94)70365-5. [DOI] [PubMed] [Google Scholar]
- Hoppe J. E. Methods for isolation of Bordetella pertussis from patients with whooping cough. Eur J Clin Microbiol Infect Dis. 1988 Oct;7(5):616–620. doi: 10.1007/BF01964238. [DOI] [PubMed] [Google Scholar]
- Kurzynski T. A., Boehm D. M., Rott-Petri J. A., Schell R. F., Allison P. E. Comparison of modified Bordet-Gengou and modified Regan-Lowe media for the isolation of Bordetella pertussis and Bordetella parapertussis. J Clin Microbiol. 1988 Dec;26(12):2661–2663. doi: 10.1128/jcm.26.12.2661-2663.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mertsola J., Ruuskanen O., Kuronen T., Meurman O., Viljanen M. K. Serologic diagnosis of pertussis: evaluation of pertussis toxin and other antigens in enzyme-linked immunosorbent assay. J Infect Dis. 1990 May;161(5):966–971. doi: 10.1093/infdis/161.5.966. [DOI] [PubMed] [Google Scholar]
- Onorato I. M., Wassilak S. G. Laboratory diagnosis of pertussis: the state of the art. Pediatr Infect Dis J. 1987 Feb;6(2):145–151. doi: 10.1097/00006454-198702000-00002. [DOI] [PubMed] [Google Scholar]
- Regan J., Lowe F. Enrichment medium for the isolation of Bordetella. J Clin Microbiol. 1977 Sep;6(3):303–309. doi: 10.1128/jcm.6.3.303-309.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van der Zee A., Agterberg C., Peeters M., Schellekens J., Mooi F. R. Polymerase chain reaction assay for pertussis: simultaneous detection and discrimination of Bordetella pertussis and Bordetella parapertussis. J Clin Microbiol. 1993 Aug;31(8):2134–2140. doi: 10.1128/jcm.31.8.2134-2140.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]