Abstract
Stools and sera collected during an experimental hepatitis E virus (HEV) infection in monkeys and collected from humans with acute HEV infections during epidemic and sporadic cases were analyzed by reverse transcription-PCR. Two methods for RNA purification were compared. Proteinase K digestion and phenolchloroform extraction were more efficient than guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.
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- Buffone G. J., Demmler G. J., Schimbor C. M., Greer J. Improved amplification of cytomegalovirus DNA from urine after purification of DNA with glass beads. Clin Chem. 1991 Nov;37(11):1945–1949. [PubMed] [Google Scholar]
- Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
- Gust I. D., Purcell R. H. Report of a workshop: waterborne non-A, non-B hepatitis. J Infect Dis. 1987 Oct;156(4):630–635. doi: 10.1093/infdis/156.4.630. [DOI] [PubMed] [Google Scholar]
- Longer C. F., Denny S. L., Caudill J. D., Miele T. A., Asher L. V., Myint K. S., Huang C. C., Engler W. F., LeDuc J. W., Binn L. N. Experimental hepatitis E: pathogenesis in cynomolgus macaques (Macaca fascicularis). J Infect Dis. 1993 Sep;168(3):602–609. doi: 10.1093/infdis/168.3.602. [DOI] [PubMed] [Google Scholar]
- Reyes G. R., Purdy M. A., Kim J. P., Luk K. C., Young L. M., Fry K. E., Bradley D. W. Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science. 1990 Mar 16;247(4948):1335–1339. doi: 10.1126/science.2107574. [DOI] [PubMed] [Google Scholar]
- Tsarev S. A., Emerson S. U., Tsareva T. S., Yarbough P. O., Lewis M., Govindarajan S., Reyes G. R., Shapiro M., Purcell R. H. Variation in course of hepatitis E in experimentally infected cynomolgus monkeys. J Infect Dis. 1993 Jun;167(6):1302–1306. doi: 10.1093/infdis/167.6.1302. [DOI] [PubMed] [Google Scholar]
- Wilde J., Eiden J., Yolken R. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol. 1990 Jun;28(6):1300–1307. doi: 10.1128/jcm.28.6.1300-1307.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]