Abstract
Anaerobic chambers and jars are the two conventional methods used in clinical microbiology laboratories to produce an anaerobic atmosphere. The evacuation-replacement method, the Oxoid AnaeroGen, the Merck Anaerocult A, the BBL GasPak, the BBL GasPakPlus, the Adams Scientific GasGendicator, the Difco Anaerobic, and the bioMérieux Generbox anaer systems were compared for the timed decrease in the oxygen concentration in an anaerobic jar. The experiment was repeated 10 times for each system. The oxygen concentration was measured with an oxygen analyzer series 3600 instrument (Orbisphere Laboratories, Neuchâtel-Geneva Switzerland). The BBL GasPak, the BBL GasPakPlus, the bioMérieux Generbox, the Adams Scientific GasGendicator, and the Difco Anaerobic systems contain sodium borohydride, which liberates hydrogen. The Anaerocult A system contains iron powder which binds the oxygen. The active ingredient of the AnaeroGen system is ascorbic acid. The times to reach an O2 concentration of 0.5% were 8 to 15 min for the evacuation-replacement method, 26 to 41 min for the AnaeroGen system, 60 to 93 min for the Anaerocult A system, and 22 to 419 min for the sodium borohydride systems. The AnaeroGen system, the Anaerocult A system, and the evacuation-replacement method never failed to produce an anaerobic atmosphere. The sodium borohydride systems failed in 10 of 70 runs. These results suggest that the evacuation-replacement method or the Oxoid AnaeroGen system should be used to produce an anaerobic atmosphere. The Anaerocult A system showed a good reproducibility, but the length of time required to reach an appropriately low level of oxygen was too long. Because of the high failure rate, the borohydride systems cannot be recommended.
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Selected References
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- Downes J., Mangels J. I., Holden J., Ferraro M. J., Baron E. J. Evaluation of two single-plate incubation systems and the anaerobic chamber for the cultivation of anaerobic bacteria. J Clin Microbiol. 1990 Feb;28(2):246–248. doi: 10.1128/jcm.28.2.246-248.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heizmann W. R., Werner H. GasPak Plus versus Anaerocult A--two carbon dioxide/hydrogen systems for cultivation of anaerobes. Zentralbl Bakteriol Mikrobiol Hyg A. 1989 Mar;270(4):511–516. doi: 10.1016/s0176-6724(89)80022-7. [DOI] [PubMed] [Google Scholar]
- Loesche W. J. Oxygen sensitivity of various anaerobic bacteria. Appl Microbiol. 1969 Nov;18(5):723–727. doi: 10.1128/am.18.5.723-727.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller P. H., Wiggs L. S., Miller J. M. Evaluation of AnaeroGen system for growth of anaerobic bacteria. J Clin Microbiol. 1995 Sep;33(9):2388–2391. doi: 10.1128/jcm.33.9.2388-2391.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Grady F., Eley A. Continuous opacity monitoring of the growth of bacteria under strict anaerobic conditions. J Clin Pathol. 1983 Nov;36(11):1229–1232. doi: 10.1136/jcp.36.11.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seip W. F., Evans G. L. Atmospheric analysis and redox potentials of culture media in the GasPak System. J Clin Microbiol. 1980 Mar;11(3):226–233. doi: 10.1128/jcm.11.3.226-233.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sisson P. R., Ingham H. R., Byrne P. O. Wise Anaerobic Work Station: an evaluation. J Clin Pathol. 1987 Mar;40(3):286–291. doi: 10.1136/jcp.40.3.286. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walden W. C., Hentges D. J. Differential effects of oxygen and oxidation-reduction potential on the multiplication of three species of anaerobic intestinal bacteria. Appl Microbiol. 1975 Nov;30(5):781–785. doi: 10.1128/am.30.5.781-785.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willemot J. M., Su W. J., Briend R., Sebald M. Balayage gazeux à l'aide d'un automate, une amélioration technique d'obtention d'une atmosphère propice à la culture en jarre des bactéries anaérobies. Ann Biol Clin (Paris) 1992;50(4):239–245. [PubMed] [Google Scholar]
- Wong G. H., Steiner B. M., Graves S. R. Effects of anaerobic and microaerophilic conditions of extraction and incubation on the survival of Treponema pallidum in vitro. Br J Vener Dis. 1982 Jun;58(3):139–142. doi: 10.1136/sti.58.3.139. [DOI] [PMC free article] [PubMed] [Google Scholar]