Abstract
A fastidious, slowly growing, spiral gram-negative bacterium was isolated from the liver abscess of an 82-year-old man with a 3-week history of febrile illness. The organism was an obligate anaerobe that grew at 37 and 42 degrees C but not at 25 degrees C. Its vibrioid or spiral morphology on Gram staining, rapid progressive motility, electron micrograph features, and biochemical tests were all consistent with the organism belonging to the genus Desulfovibrio. 16S rRNA gene sequencing of this organism demonstrated a 97% similarity to Desulfovibrio desulfuricans with 45 nucleotide differences, suggesting that it is a new species of Desulfovibrio.
Full Text
The Full Text of this article is available as a PDF (624.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barnes P. F., De Cock K. M., Reynolds T. N., Ralls P. W. A comparison of amebic and pyogenic abscess of the liver. Medicine (Baltimore) 1987 Nov;66(6):472–483. doi: 10.1097/00005792-198711000-00005. [DOI] [PubMed] [Google Scholar]
- Baron E. J., Bennion R., Thompson J., Strong C., Summanen P., McTeague M., Finegold S. M. A microbiological comparison between acute and complicated appendicitis. Clin Infect Dis. 1992 Jan;14(1):227–231. doi: 10.1093/clinids/14.1.227. [DOI] [PubMed] [Google Scholar]
- Beerens H., Romond C. Sulfate-reducing anaerobic bacteria in human feces. Am J Clin Nutr. 1977 Nov;30(11):1770–1776. doi: 10.1093/ajcn/30.11.1770. [DOI] [PubMed] [Google Scholar]
- Devereux R., He S. H., Doyle C. L., Orkland S., Stahl D. A., LeGall J., Whitman W. B. Diversity and origin of Desulfovibrio species: phylogenetic definition of a family. J Bacteriol. 1990 Jul;172(7):3609–3619. doi: 10.1128/jb.172.7.3609-3619.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox J. G., Dewhirst F. E., Fraser G. J., Paster B. J., Shames B., Murphy J. C. Intracellular Campylobacter-like organism from ferrets and hamsters with proliferative bowel disease is a Desulfovibrio sp. J Clin Microbiol. 1994 May;32(5):1229–1237. doi: 10.1128/jcm.32.5.1229-1237.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fry N. K., Warwick S., Saunders N. A., Embley T. M. The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae. J Gen Microbiol. 1991 May;137(5):1215–1222. doi: 10.1099/00221287-137-5-1215. [DOI] [PubMed] [Google Scholar]
- Gebhart C. J., Barns S. M., McOrist S., Lin G. F., Lawson G. H. Ileal symbiont intracellularis, an obligate intracellular bacterium of porcine intestines showing a relationship to Desulfovibrio species. Int J Syst Bacteriol. 1993 Jul;43(3):533–538. doi: 10.1099/00207713-43-3-533. [DOI] [PubMed] [Google Scholar]
- Gibson G. R., Cummings J. H., Macfarlane G. T. Competition for hydrogen between sulphate-reducing bacteria and methanogenic bacteria from the human large intestine. J Appl Bacteriol. 1988 Sep;65(3):241–247. doi: 10.1111/j.1365-2672.1988.tb01891.x. [DOI] [PubMed] [Google Scholar]
- Gibson G. R., Macfarlane G. T., Cummings J. H. Occurrence of sulphate-reducing bacteria in human faeces and the relationship of dissimilatory sulphate reduction to methanogenesis in the large gut. J Appl Bacteriol. 1988 Aug;65(2):103–111. doi: 10.1111/j.1365-2672.1988.tb01498.x. [DOI] [PubMed] [Google Scholar]
- Howard B. H., Hungate R. E. Desulfovibrio of the sheep rumen. Appl Environ Microbiol. 1976 Oct;32(4):598–602. doi: 10.1128/aem.32.4.598-602.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson C. C., Finegold S. M. Uncommonly encountered, motile, anaerobic gram-negative bacilli associated with infection. Rev Infect Dis. 1987 Nov-Dec;9(6):1150–1162. doi: 10.1093/clinids/9.6.1150. [DOI] [PubMed] [Google Scholar]
- Larsen N., Olsen G. J., Maidak B. L., McCaughey M. J., Overbeek R., Macke T. J., Marsh T. L., Woese C. R. The ribosomal database project. Nucleic Acids Res. 1993 Jul 1;21(13):3021–3023. doi: 10.1093/nar/21.13.3021. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lawson G. H., McOrist S., Jasni S., Mackie R. A. Intracellular bacteria of porcine proliferative enteropathy: cultivation and maintenance in vitro. J Clin Microbiol. 1993 May;31(5):1136–1142. doi: 10.1128/jcm.31.5.1136-1142.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maidak B. L., Larsen N., McCaughey M. J., Overbeek R., Olsen G. J., Fogel K., Blandy J., Woese C. R. The Ribosomal Database Project. Nucleic Acids Res. 1994 Sep;22(17):3485–3487. doi: 10.1093/nar/22.17.3485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martinez-Murcia A. J., Benlloch S., Collins M. D. Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Int J Syst Bacteriol. 1992 Jul;42(3):412–421. doi: 10.1099/00207713-42-3-412. [DOI] [PubMed] [Google Scholar]
- McDonald M. I., Corey G. R., Gallis H. A., Durack D. T. Single and multiple pyogenic liver abscesses. Natural history, diagnosis and treatment, with emphasis on percutaneous drainage. Medicine (Baltimore) 1984 Sep;63(5):291–302. doi: 10.1097/00005792-198409000-00003. [DOI] [PubMed] [Google Scholar]
- Olsen G. J., Matsuda H., Hagstrom R., Overbeek R. fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci. 1994 Feb;10(1):41–48. doi: 10.1093/bioinformatics/10.1.41. [DOI] [PubMed] [Google Scholar]
- Perera M. R., Kirk A., Noone P. Presentation, diagnosis and management of liver abscess. Lancet. 1980 Sep 20;2(8195 Pt 1):629–632. doi: 10.1016/s0140-6736(80)90293-7. [DOI] [PubMed] [Google Scholar]
- Porschen R. K., Chan P. Anaerobic vibrio-like organisms cultured from blood: Desulfovibrio desulfuricans and Succinivibrio species. J Clin Microbiol. 1977 Apr;5(4):444–447. doi: 10.1128/jcm.5.4.444-447.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Relman D. A., Loutit J. S., Schmidt T. M., Falkow S., Tompkins L. S. The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens. N Engl J Med. 1990 Dec 6;323(23):1573–1580. doi: 10.1056/NEJM199012063232301. [DOI] [PubMed] [Google Scholar]
- Relman D. A., Schmidt T. M., MacDermott R. P., Falkow S. Identification of the uncultured bacillus of Whipple's disease. N Engl J Med. 1992 Jul 30;327(5):293–301. doi: 10.1056/NEJM199207303270501. [DOI] [PubMed] [Google Scholar]
- Rubin R. H., Swartz M. N., Malt R. Hepatic abscess: changes in clinical, bacteriologic and therapeutic aspects. Am J Med. 1974 Oct;57(4):601–610. doi: 10.1016/0002-9343(74)90012-6. [DOI] [PubMed] [Google Scholar]
- Sabbaj J., Sutter V. L., Finegold S. M. Anaerobic pyogenic liver abscess. Ann Intern Med. 1972 Oct;77(4):627–638. doi: 10.7326/0003-4819-77-4-629. [DOI] [PubMed] [Google Scholar]
- Solnick J. V., O'Rourke J., Lee A., Paster B. J., Dewhirst F. E., Tompkins L. S. An uncultured gastric spiral organism is a newly identified Helicobacter in humans. J Infect Dis. 1993 Aug;168(2):379–385. doi: 10.1093/infdis/168.2.379. [DOI] [PubMed] [Google Scholar]