EVOLUTION. For the article “Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae,” by Yasuko Matsumoto, Tsuyoshi Nakano, Masafumi Yamamoto, Yuko Matsushima-Hibiya, Ken-Ichi Odagiri, Osamu Yata, Kotaro Koyama, Takashi Sugimura, and Keiji Wakabayashi, which appeared in issue 7, February 19, 2008, of Proc Natl Acad Sci USA (105:2516–2520; first published February 6, 2008; 10.1073/pnas.0712221105), the authors note that in Table 1, columns 6 and 7 carried incorrect footnote symbols. These columns should carry the † footnote symbol, corresponding to: “–, No detection of cytotoxicity, DNA ADP-ribosylating activity, specific bands recognized by anti-pierisin-1 antibodies and amplification of NAD-binding site sequence by PCR. +, Detection of specific bands recognized by the antibodies and amplification of NAD-binding site sequence by PCR.” The corrected table appears below.
Table 1.
Pierisin-like activity in crude extracts from various butterflies, and areas where the samples were collected
| Species | Concentration of 50% cell viability, ng/ml*† |
DNA ADP- ribosylating activity, pg/μg†‡ |
Western blot† | PCR† | Collection area | ||
|---|---|---|---|---|---|---|---|
| Fifth larvae | Pupae | Adults | Adults | ||||
| Pieris rapae | 24.3 (3.6–38.9) | 23.9 (10.8–43.0) | 691 (428–812) | 22.88 | + | + | Tochigi, Japan |
| Pieris canidia | 4.5 (3.8–5.1) | 3.9 (2.3–4.1) | 53 (36–165) | 263.71 | + | + | Okinawa, Japan |
| Pieris napi | 9.0 (5.0–10.7) | 11.5 (10.7–77.4) | 109 (29–134) | 14.47 | + | + | Tochigi, Japan |
| Pieris melete | 22.4 (3.6–39.4) | 272 (77–308) | 649 (129–4,380) | 37.01 | + | + | Tochigi, Japan |
| Pieris brassicae | 16.6 (3.1–40.1) | 1.8 (1.7–2.3) | 123 (43–147) | 113.26 | + | + | Hokkaido, Japan |
| Pontia daplidice | 53.8 (35–75) | 86 (59–107) | 450 (409–908) | 44.92 | + | + | Gyeonggi, Korea |
| Talbotia naganum§ | N.E. | N.E. | 10,000 (5,100–10,100) | 19.21 | + | + | Nan hin, Laos |
| Aporia gigantea§ | N.E. | N.E. | 34 (31–340) | 419.1 | + | + | Hagian, Vietnam |
| Aporia crataegi | 4.5 (2.9–6.5) | 18.1 (11.5–22.3) | 77 (38–146) | 29.35 | + | + | Hokkaido, Japan |
| Aporia hippia§ | N.E. | N.E. | 78 (11–159) | 31.46 | + | + | Shaanxi, China |
| Delias pasithoe§ | N.E. | N.E. | 19,300 (14,600–23,200) | 95.36 | + | + | Hong Kong, China |
| Appias nero§ | N.E. | N.E. | 39,300 (7,000–45,200) | 2.55 | + | + | Nan hin, Laos |
| Appias paulina | 1,255 (1,210–2,040) | 10,560 (10,400–18,500) | 38,400 (30,000–40,600) | 3.45 | + | + | Okinawa, Japan |
| Appias lyncida | – | – | − | – | – | − | Okinawa, Japan |
| Leptosia nina | N.E. | N.E. | − | – | – | − | Okinawa, Japan |
| Anthocharis scolymus | – | – | − | – | – | − | Tochigi, Japan |
| Eurema hecabe | – | – | − | – | – | − | Tochigi, Japan |
| Catopsilia pomona | N.E. | N.E. | − | – | – | − | Okinawa, Japan |
| Catopsilia scylla | N.E. | – | − | – | – | − | Malaysia |
| Colias erate | – | – | − | – | – | − | Tochigi, Japan |
*Median values of 50% cell viability (minimum-maximum) are shown. The data were obtained from three independent assays by using two wells for each assay. N.E., not examined.
†−, No detection of cytotoxicity, DNA ADP-ribosylating activity, specific bands recognized by anti-pierisin-1 antibodies and amplification of NAD-binding site sequence by PCR. +, Detection of specific bands recognized by the antibodies and amplification of NAD-binding site sequence by PCR.
‡DNA ADP-ribosylating activities expressed as picograms of pierisin-1 per microgram of protein.
§Samples extracted from dried butterflies.