Abstract
Differences between isotypes of monoclonal antibodies were employed to detect influenza A and B viruses and respiratory syncytial virus by direct immunofluorescence using fluorescein isothiocyanate or Texas Red conjugates. Examination of 56 specimens for influenza A and B viruses and 112 specimens for influenza A virus and respiratory syncytial virus showed the mixed-isotype test to be comparable to the conventional procedure.
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- Brumback B. G., Farthing P. G., Castellino S. N. Simultaneous detection of and differentiation between herpes simplex and varicella-zoster viruses with two fluorescent probes in the same test system. J Clin Microbiol. 1993 Dec;31(12):3260–3263. doi: 10.1128/jcm.31.12.3260-3263.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brumback B. G., Wade C. D. Simultaneous culture for adenovirus, cytomegalovirus, and herpes simplex virus in same shell vial by using three-color fluorescence. J Clin Microbiol. 1994 Sep;32(9):2289–2290. doi: 10.1128/jcm.32.9.2289-2290.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Denny F. W., Clyde W. A., Jr Acute lower respiratory tract infections in nonhospitalized children. J Pediatr. 1986 May;108(5 Pt 1):635–646. doi: 10.1016/s0022-3476(86)81034-4. [DOI] [PubMed] [Google Scholar]
- Dolin R., Reichman R. C., Madore H. P., Maynard R., Linton P. N., Webber-Jones J. A controlled trial of amantadine and rimantadine in the prophylaxis of influenza A infection. N Engl J Med. 1982 Sep 2;307(10):580–584. doi: 10.1056/NEJM198209023071002. [DOI] [PubMed] [Google Scholar]
- Dominguez E. A., Taber L. H., Couch R. B. Comparison of rapid diagnostic techniques for respiratory syncytial and influenza A virus respiratory infections in young children. J Clin Microbiol. 1993 Sep;31(9):2286–2290. doi: 10.1128/jcm.31.9.2286-2290.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Espy M. J., Smith T. F., Harmon M. W., Kendal A. P. Rapid detection of influenza virus by shell vial assay with monoclonal antibodies. J Clin Microbiol. 1986 Oct;24(4):677–679. doi: 10.1128/jcm.24.4.677-679.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fleming D. M., Cross K. W. Respiratory syncytial virus or influenza? Lancet. 1993 Dec 18;342(8886-8887):1507–1510. doi: 10.1016/s0140-6736(05)80082-0. [DOI] [PubMed] [Google Scholar]
- Hall C. B., McBride J. T., Walsh E. E., Bell D. M., Gala C. L., Hildreth S., Ten Eyck L. G., Hall W. J. Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. A randomized double-blind study. N Engl J Med. 1983 Jun 16;308(24):1443–1447. doi: 10.1056/NEJM198306163082403. [DOI] [PubMed] [Google Scholar]
- Stokes C. E., Bernstein J. M., Kyger S. A., Hayden F. G. Rapid diagnosis of influenza A and B by 24-h fluorescent focus assays. J Clin Microbiol. 1988 Jul;26(7):1263–1266. doi: 10.1128/jcm.26.7.1263-1266.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swierkosz E. M., Flanders R., Melvin L., Miller J. D., Kline M. W. Evaluation of the Abbott TESTPACK RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens. J Clin Microbiol. 1989 Jun;27(6):1151–1154. doi: 10.1128/jcm.27.6.1151-1154.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Todd S. J., Minnich L., Waner J. L. Comparison of rapid immunofluorescence procedure with TestPack RSV and Directigen FLU-A for diagnosis of respiratory syncytial virus and influenza A virus. J Clin Microbiol. 1995 Jun;33(6):1650–1651. doi: 10.1128/jcm.33.6.1650-1651.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waner J. L. Mixed viral infections: detection and management. Clin Microbiol Rev. 1994 Apr;7(2):143–151. doi: 10.1128/cmr.7.2.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waner J. L., Todd S. J., Shalaby H., Murphy P., Wall L. V. Comparison of Directigen FLU-A with viral isolation and direct immunofluorescence for the rapid detection and identification of influenza A virus. J Clin Microbiol. 1991 Mar;29(3):479–482. doi: 10.1128/jcm.29.3.479-482.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waner J. L., Whitehurst N. J., Todd S. J., Shalaby H., Wall L. V. Comparison of directigen RSV with viral isolation and direct immunofluorescence for the identification of respiratory syncytial virus. J Clin Microbiol. 1990 Mar;28(3):480–483. doi: 10.1128/jcm.28.3.480-483.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]