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Journal of Biomolecular Techniques : JBT logoLink to Journal of Biomolecular Techniques : JBT
. 1999 Mar;10(1):11–16.

Quantitative real-time polymerase chain reaction for the core facility using TaqMan and the Perkin-Elmer/Applied Biosystems Division 7700 Sequence Detector

D Grove
PMCID: PMC2291580  PMID: 19499001

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Selected References

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  1. Araki R., Fujimori A., Hamatani K., Mita K., Saito T., Mori M., Fukumura R., Morimyo M., Muto M., Itoh M. Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice. Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2438–2443. doi: 10.1073/pnas.94.6.2438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Batt C. A. Molecular diagnostics for dairy-borne pathogens. J Dairy Sci. 1997 Jan;80(1):220–229. doi: 10.3168/jds.S0022-0302(97)75931-9. [DOI] [PubMed] [Google Scholar]
  3. Brandt M. E., Padhye A. A., Mayer L. W., Holloway B. P. Utility of random amplified polymorphic DNA PCR and TaqMan automated detection in molecular identification of Aspergillus fumigatus. J Clin Microbiol. 1998 Jul;36(7):2057–2062. doi: 10.1128/jcm.36.7.2057-2062.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Casteels K. M., Mathieu C., Waer M., Valckx D., Overbergh L., Laureys J. M., Bouillon R. Prevention of type I diabetes in nonobese diabetic mice by late intervention with nonhypercalcemic analogs of 1,25-dihydroxyvitamin D3 in combination with a short induction course of cyclosporin A. Endocrinology. 1998 Jan;139(1):95–102. doi: 10.1210/endo.139.1.5641. [DOI] [PubMed] [Google Scholar]
  5. Chen S., Yee A., Griffiths M., Larkin C., Yamashiro C. T., Behari R., Paszko-Kolva C., Rahn K., De Grandis S. A. The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities. Int J Food Microbiol. 1997 Apr 15;35(3):239–250. doi: 10.1016/s0168-1605(97)01241-5. [DOI] [PubMed] [Google Scholar]
  6. Desjardin L. E., Chen Y., Perkins M. D., Teixeira L., Cave M. D., Eisenach K. D. Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis. J Clin Microbiol. 1998 Jul;36(7):1964–1968. doi: 10.1128/jcm.36.7.1964-1968.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gelmini S., Orlando C., Sestini R., Vona G., Pinzani P., Ruocco L., Pazzagli M. Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification. Clin Chem. 1997 May;43(5):752–758. [PubMed] [Google Scholar]
  8. Gerard C. J., Arboleda M. J., Solar G., Mulé J. J., Kerr W. G. A rapid and quantitative assay to estimate gene transfer into retrovirally transduced hematopoietic stem/progenitor cells using a 96-well format PCR and fluorescent detection system universal for MMLV-based proviruses. Hum Gene Ther. 1996 Feb 10;7(3):343–354. doi: 10.1089/hum.1996.7.3-343. [DOI] [PubMed] [Google Scholar]
  9. Gerard C. J., Olsson K., Ramanathan R., Reading C., Hanania E. G. Improved quantitation of minimal residual disease in multiple myeloma using real-time polymerase chain reaction and plasmid-DNA complementarity determining region III standards. Cancer Res. 1998 Sep 1;58(17):3957–3964. [PubMed] [Google Scholar]
  10. Gibson U. E., Heid C. A., Williams P. M. A novel method for real time quantitative RT-PCR. Genome Res. 1996 Oct;6(10):995–1001. doi: 10.1101/gr.6.10.995. [DOI] [PubMed] [Google Scholar]
  11. Heid C. A., Stevens J., Livak K. J., Williams P. M. Real time quantitative PCR. Genome Res. 1996 Oct;6(10):986–994. doi: 10.1101/gr.6.10.986. [DOI] [PubMed] [Google Scholar]
  12. Higgins J. A., Ezzell J., Hinnebusch B. J., Shipley M., Henchal E. A., Ibrahim M. S. 5' nuclease PCR assay to detect Yersinia pestis. J Clin Microbiol. 1998 Aug;36(8):2284–2288. doi: 10.1128/jcm.36.8.2284-2288.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holland P. M., Abramson R. D., Watson R., Gelfand D. H. Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7276–7280. doi: 10.1073/pnas.88.16.7276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kruse N., Pette M., Toyka K., Rieckmann P. Quantification of cytokine mRNA expression by RT PCR in samples of previously frozen blood. J Immunol Methods. 1997 Dec 29;210(2):195–203. doi: 10.1016/s0022-1759(97)00188-9. [DOI] [PubMed] [Google Scholar]
  15. Livak K. J., Flood S. J., Marmaro J., Giusti W., Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 1995 Jun;4(6):357–362. doi: 10.1101/gr.4.6.357. [DOI] [PubMed] [Google Scholar]
  16. Lo Y. M., Tein M. S., Lau T. K., Haines C. J., Leung T. N., Poon P. M., Wainscoat J. S., Johnson P. J., Chang A. M., Hjelm N. M. Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis. Am J Hum Genet. 1998 Apr;62(4):768–775. doi: 10.1086/301800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lockey C., Otto E., Long Z. Real-time fluorescence detection of a single DNA molecule. Biotechniques. 1998 May;24(5):744–746. doi: 10.2144/98245bm09. [DOI] [PubMed] [Google Scholar]
  18. Luoh S. M., Di Marco F., Levin N., Armanini M., Xie M. H., Nelson C., Bennett G. L., Williams M., Spencer S. A., Gurney A. Cloning and characterization of a human leptin receptor using a biologically active leptin immunoadhesin. J Mol Endocrinol. 1997 Feb;18(1):77–85. doi: 10.1677/jme.0.0180077. [DOI] [PubMed] [Google Scholar]
  19. Marcucci G., Livak K. J., Bi W., Strout M. P., Bloomfield C. D., Caligiuri M. A. Detection of minimal residual disease in patients with AML1/ETO-associated acute myeloid leukemia using a novel quantitative reverse transcription polymerase chain reaction assay. Leukemia. 1998 Sep;12(9):1482–1489. doi: 10.1038/sj.leu.2401128. [DOI] [PubMed] [Google Scholar]
  20. McGoldrick A., Lowings J. P., Ibata G., Sands J. J., Belak S., Paton D. J. A novel approach to the detection of classical swine fever virus by RT-PCR with a fluorogenic probe (TaqMan). J Virol Methods. 1998 Jun;72(2):125–135. doi: 10.1016/s0166-0934(97)00208-5. [DOI] [PubMed] [Google Scholar]
  21. Mensink E., van de Locht A., Schattenberg A., Linders E., Schaap N., Geurts van Kessel A., De Witte T. Quantitation of minimal residual disease in Philadelphia chromosome positive chronic myeloid leukaemia patients using real-time quantitative RT-PCR. Br J Haematol. 1998 Aug;102(3):768–774. doi: 10.1046/j.1365-2141.1998.00823.x. [DOI] [PubMed] [Google Scholar]
  22. Morris T., Robertson B., Gallagher M. Rapid reverse transcription-PCR detection of hepatitis C virus RNA in serum by using the TaqMan fluorogenic detection system. J Clin Microbiol. 1996 Dec;34(12):2933–2936. doi: 10.1128/jcm.34.12.2933-2936.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shimokawa T., Kato M., Ezaki O., Hashimoto S. Transcriptional regulation of muscle-specific genes during myoblast differentiation. Biochem Biophys Res Commun. 1998 May 8;246(1):287–292. doi: 10.1006/bbrc.1998.8600. [DOI] [PubMed] [Google Scholar]
  24. Suryanarayana K., Wiltrout T. A., Vasquez G. M., Hirsch V. M., Lifson J. D. Plasma SIV RNA viral load determination by real-time quantification of product generation in reverse transcriptase-polymerase chain reaction. AIDS Res Hum Retroviruses. 1998 Jan 20;14(2):183–189. doi: 10.1089/aid.1998.14.183. [DOI] [PubMed] [Google Scholar]
  25. Swan D. C., Tucker R. A., Holloway B. P., Icenogle J. P. A sensitive, type-specific, fluorogenic probe assay for detection of human papillomavirus DNA. J Clin Microbiol. 1997 Apr;35(4):886–891. doi: 10.1128/jcm.35.4.886-891.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Witham P. K., Yamashiro C. T., Livak K. J., Batt C. A. A PCR-based assay for the detection of Escherichia coli Shiga-like toxin genes in ground beef. Appl Environ Microbiol. 1996 Apr;62(4):1347–1353. doi: 10.1128/aem.62.4.1347-1353.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. de Kok J. B., Hendriks J. C., van Solinge W. W., Willems H. L., Mensink E. J., Swinkels D. W. Use of real-time quantitative PCR to compare DNA isolation methods. Clin Chem. 1998 Oct;44(10):2201–2204. [PubMed] [Google Scholar]

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