Skip to main content
Journal of Biomolecular Techniques : JBT logoLink to Journal of Biomolecular Techniques : JBT
. 2001 Mar;12(1):1–3.

Multiplex, fluorescent single-strand conformation polymorphism using stepped polymerase chain reaction primers

R Scholl, A Walker, L Ballard
PMCID: PMC2291648  PMID: 19499062

Full Text

The Full Text of this article is available as a PDF (59.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brownstein M. J., Carpten J. D., Smith J. R. Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques. 1996 Jun;20(6):1004-6, 1008-10. doi: 10.2144/96206st01. [DOI] [PubMed] [Google Scholar]
  2. Emi M., Matsushima M., Katagiri T., Yoshimoto M., Kasumi F., Yokota T., Nakata T., Miki Y., Nakamura Y. Multiplex mutation screening of the BRCA1 gene in 1000 Japanese breast cancers. Jpn J Cancer Res. 1998 Jan;89(1):12–16. doi: 10.1111/j.1349-7006.1998.tb00472.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Makino R., Yazyu H., Kishimoto Y., Sekiya T., Hayashi K. F-SSCP: fluorescence-based polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. PCR Methods Appl. 1992 Aug;2(1):10–13. doi: 10.1101/gr.2.1.10. [DOI] [PubMed] [Google Scholar]
  4. Orita M., Suzuki Y., Sekiya T., Hayashi K. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics. 1989 Nov;5(4):874–879. doi: 10.1016/0888-7543(89)90129-8. [DOI] [PubMed] [Google Scholar]
  5. Ozçelik H., Andrulis I. L. Multiplex PCR-SSCP for simultaneous screening for mutations in several exons of p53. Biotechniques. 1995 May;18(5):742–744. [PubMed] [Google Scholar]
  6. Winterpacht A., Hilbert K., Schwarze U., Zabel B. Non-radioactive multiplex-SSCP analysis: detection of a new type II procollagen gene (COL2A1) mutation. Hum Genet. 1995 Apr;95(4):437–439. doi: 10.1007/BF00208971. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Biomolecular Techniques : JBT are provided here courtesy of The Association of Biomolecular Resource Facilities

RESOURCES