Abstract
A dot-immunogold filtration assay (DIGFA) for the rapid detection of reaginic antibody in the serum of syphilitic patients was developed. The assay was simple, rapid, and reproducible. The test completion time was 2 min, and the assay required no equipment. The positive dot was very obvious, and the results could easily be determined with the naked eye. A total of 350 serum samples were examined by DIGFA, the rapid plasma reagin test, and the fluorescent treponemal antibody-absorption test. The levels of agreement between DIGFA and the rapid reagin test and between DIGFA and the fluorescent treponemal antibody-absorption test were 100 and 98%, respectively. The results of clinical application indicated that DIGFA could be used as a routine screening test for syphilis.
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Selected References
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- Coffey E. M., Bradford L. L., Naritomi L. S., Wood R. M. Evaluation of the qualitative and automated quantitative microhemagglutination assay for antibodies to Treponema pallidum. Appl Microbiol. 1972 Jul;24(1):26–30. doi: 10.1128/am.24.1.26-30.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horisberger M., Clerc M. F. Labelling of colloidal gold with protein A. A quantitative study. Histochemistry. 1985;82(3):219–223. doi: 10.1007/BF00501398. [DOI] [PubMed] [Google Scholar]
- Wentworth B. B., Thompson M. A., Peter C. R., Bawdon R. E., Wilson D. L. Comparison of a hemagglutination treponemal test for syphilis (HATTS) with other serologic methods for the diagnosis of syphilis. Sex Transm Dis. 1978 Jul-Sep;5(3):103–114. doi: 10.1097/00007435-197807000-00005. [DOI] [PubMed] [Google Scholar]