Abstract
The present multicenter study proposes broth microdilution quality control (QC) ranges for the antimicrobial agents ceftiofur, enrofloxacin, florfenicol, penicillin G-novobiocin, pirlimycin, premafloxacin, and spectinomycin, which are used in veterinary practice. Six separate laboratories tested replicates of National Committee for Clinical Laboratory Standards (NCCLS)-recommended QC organisms (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, and Enterococcus faecalis ATCC 29212) on medium lots both common and unique to all laboratories. The proposed ranges were within 3 or 4 log2 dilution steps of the modal MICs for all organism-antimicrobial agent pairs, depending on their MIC distributions. With > or = 94.7% of all MIC results being within the proposed QC ranges, all combinations tested comply with NCCLS guidelines and all have been accepted by the NCCLS subcommittee developing susceptibility testing procedures for veterinary laboratories.
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Selected References
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- Barry A. L., Fuchs P. C., Jones R. N. Statistical criteria for selecting quality control limits for broth microdilution susceptibility tests with 39 different antimicrobial agents. Collaborative Antimicrobial Susceptibility Testing Group. Diagn Microbiol Infect Dis. 1989 Sep-Oct;12(5):413–420. doi: 10.1016/0732-8893(89)90112-0. [DOI] [PubMed] [Google Scholar]
- Birkenmeyer R. D., Kroll S. J., Lewis C., Stern K. F., Zurenko G. E. Synthesis and antimicrobial activity of clindamycin analogues: pirlimycin, 1,2 a potent antibacterial agent. J Med Chem. 1984 Feb;27(2):216–223. doi: 10.1021/jm00368a020. [DOI] [PubMed] [Google Scholar]
- Hamdy A. H., Olds N. L., Roberts B. J. Activity of penicillin and novobiocin against bovine mastitis pathogens. Am J Vet Res. 1975 Mar;36(3):259–262. [PubMed] [Google Scholar]
- Salmon S. A., Watts J. L., Case C. A., Hoffman L. J., Wegener H. C., Yancey R. J., Jr Comparison of MICs of ceftiofur and other antimicrobial agents against bacterial pathogens of swine from the United States, Canada, and Denmark. J Clin Microbiol. 1995 Sep;33(9):2435–2444. doi: 10.1128/jcm.33.9.2435-2444.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shryock T. R., White D. W., Werner C. S., Staples J. M. Proposed quality control guidelines for antimicrobial susceptibility tests using tilmicosin. J Clin Microbiol. 1995 Feb;33(2):331–335. doi: 10.1128/jcm.33.2.331-335.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Syriopoulou V. P., Harding A. L., Goldmann D. A., Smith A. L. In vitro antibacterial activity of fluorinated analogs of chloramphenicol and thiamphenicol. Antimicrob Agents Chemother. 1981 Feb;19(2):294–297. doi: 10.1128/aac.19.2.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zurenko G. E., Yagi B. H., Vavra J. J., Wentworth B. B. In vitro antibacterial activity of trospectomycin (U-63366F), a novel spectinomycin analog. Antimicrob Agents Chemother. 1988 Feb;32(2):216–223. doi: 10.1128/aac.32.2.216. [DOI] [PMC free article] [PubMed] [Google Scholar]