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. 1996 Sep;34(9):2066–2071. doi: 10.1128/jcm.34.9.2066-2071.1996

Molecular epidemiology of Mycobacterium bovis in Texas and Mexico.

V S Perumaalla 1, L G Adams 1, J B Payeur 1, J L Jarnagin 1, D R Baca 1, F Suárez Güemes 1, T A Ficht 1
PMCID: PMC229191  PMID: 8862559

Abstract

Seventy-nine Mycobacterium bovis isolates recovered from Mexican and Texas cattle were categorized into 16 and 25 distinct types on the basis of IS6110 and direct-repeat fingerprint patterns, respectively. By using a combination of both fingerprint patterns, 30 distinct restriction fragment length polymorphism types were defined. Fifty-eight of 79 isolates (73%) were distributed among nine clusters. Clustered isolates were identified within herds, as well as in geographically disperse herds in Texas and Mexico. This observation is consistent with active transmission within herds and among herds, presumably as a result of active or historical cattle movements. The majority of bovine isolates (64 of 79) exhibited a single copy of IS6110. Interestingly, in contrast to previous studies, a high percentage of bovine isolates (15 of 79) exhibited multiple IS6110 copies (two to five) distributed among 11 different restriction fragment length polymorphism types. It is speculated that transmission from noncattle sources may be responsible. Continued fingerprinting of isolates originating from nonbovine sources and herd surveys is expected to provide useful information regarding the epidemiology of tuberculosis in this region.

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Selected References

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  1. Collins C. H., Grange J. M. The bovine tubercle bacillus. J Appl Bacteriol. 1983 Aug;55(1):13–29. doi: 10.1111/j.1365-2672.1983.tb02643.x. [DOI] [PubMed] [Google Scholar]
  2. Collins D. M., De Lisle G. W., Gabric D. M. Geographic distribution of restriction types of Mycobacterium bovis isolates from brush-tailed possums (Trichosurus vulpecula) in New Zealand. J Hyg (Lond) 1986 Jun;96(3):431–438. doi: 10.1017/s0022172400066201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collins D. M., Erasmuson S. K., Stephens D. M., Yates G. F., De Lisle G. W. DNA fingerprinting of Mycobacterium bovis strains by restriction fragment analysis and hybridization with insertion elements IS1081 and IS6110. J Clin Microbiol. 1993 May;31(5):1143–1147. doi: 10.1128/jcm.31.5.1143-1147.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins D. M., Gabric D. M., de Lisle G. W. Typing of Mycobacterium bovis isolates from cattle and other animals in the same locality. N Z Vet J. 1988 Mar;36(1):45–46. doi: 10.1080/00480169.1988.35476. [DOI] [PubMed] [Google Scholar]
  5. Eisenach K. D., Crawford J. T., Bates J. H. Genetic relatedness among strains of the Mycobacterium tuberculosis complex. Analysis of restriction fragment heterogeneity using cloned DNA probes. Am Rev Respir Dis. 1986 Jun;133(6):1065–1068. doi: 10.1164/arrd.1986.133.6.1065. [DOI] [PubMed] [Google Scholar]
  6. Essey M. A., Koller M. A. Status of bovine tuberculosis in North America. Vet Microbiol. 1994 May;40(1-2):15–22. doi: 10.1016/0378-1135(94)90043-4. [DOI] [PubMed] [Google Scholar]
  7. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  8. Frothingham R., Hills H. G., Wilson K. H. Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex. J Clin Microbiol. 1994 Jul;32(7):1639–1643. doi: 10.1128/jcm.32.7.1639-1643.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gross W. M., Hawkins J. E. Radiometric selective inhibition tests for differentiation of Mycobacterium tuberculosis, Mycobacterium bovis, and other mycobacteria. J Clin Microbiol. 1985 Apr;21(4):565–568. doi: 10.1128/jcm.21.4.565-568.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gutiérrez M., Samper S., Gavigan J. A., García Marín J. F., Martín C. Differentiation by molecular typing of Mycobacterium bovis strains causing tuberculosis in cattle and goats. J Clin Microbiol. 1995 Nov;33(11):2953–2956. doi: 10.1128/jcm.33.11.2953-2956.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hermans P. W., van Soolingen D., Bik E. M., de Haas P. E., Dale J. W., van Embden J. D. Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains. Infect Immun. 1991 Aug;59(8):2695–2705. doi: 10.1128/iai.59.8.2695-2705.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mazurek G. H., Cave M. D., Eisenach K. D., Wallace R. J., Jr, Bates J. H., Crawford J. T. Chromosomal DNA fingerprint patterns produced with IS6110 as strain-specific markers for epidemiologic study of tuberculosis. J Clin Microbiol. 1991 Sep;29(9):2030–2033. doi: 10.1128/jcm.29.9.2030-2033.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Neill S. D., Pollock J. M., Bryson D. B., Hanna J. Pathogenesis of Mycobacterium bovis infection in cattle. Vet Microbiol. 1994 May;40(1-2):41–52. doi: 10.1016/0378-1135(94)90045-0. [DOI] [PubMed] [Google Scholar]
  14. Ross B. C., Raios K., Jackson K., Dwyer B. Molecular cloning of a highly repeated DNA element from Mycobacterium tuberculosis and its use as an epidemiological tool. J Clin Microbiol. 1992 Apr;30(4):942–946. doi: 10.1128/jcm.30.4.942-946.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roychoudhury R., Wu R. Terminal transferase-catalyzed addition of nucleotides to the 3' termini of DNA. Methods Enzymol. 1980;65(1):43–62. doi: 10.1016/s0076-6879(80)65009-5. [DOI] [PubMed] [Google Scholar]
  16. Skuce R. A., Brittain D., Hughes M. S., Beck L. A., Neill S. D. Genomic fingerprinting of Mycobacterium bovis from cattle by restriction fragment length polymorphism analysis. J Clin Microbiol. 1994 Oct;32(10):2387–2392. doi: 10.1128/jcm.32.10.2387-2392.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Szewzyk R., Svenson S. B., Hoffner S. E., Bölske G., Wahlström H., Englund L., Engvall A., Källenius G. Molecular epidemiological studies of Mycobacterium bovis infections in humans and animals in Sweden. J Clin Microbiol. 1995 Dec;33(12):3183–3185. doi: 10.1128/jcm.33.12.3183-3185.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Thoen C. O., Quinn W. J., Miller L. D., Stackhouse L. L., Newcomb B. F., Ferrell J. M. Mycobacterium bovis infection in North American elk (Cervus elaphus). J Vet Diagn Invest. 1992 Oct;4(4):423–427. doi: 10.1177/104063879200400410. [DOI] [PubMed] [Google Scholar]
  19. Thoen C. O., Throlson K. J., Miller L. D., Himes E. M., Morgan R. L. Pathogenesis of Mycobacterium bovis infection in American bison. Am J Vet Res. 1988 Nov;49(11):1861–1865. [PubMed] [Google Scholar]
  20. van Embden J. D., Cave M. D., Crawford J. T., Dale J. W., Eisenach K. D., Gicquel B., Hermans P., Martin C., McAdam R., Shinnick T. M. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol. 1993 Feb;31(2):406–409. doi: 10.1128/jcm.31.2.406-409.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van Soolingen D., Hermans P. W., de Haas P. E., Soll D. R., van Embden J. D. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol. 1991 Nov;29(11):2578–2586. doi: 10.1128/jcm.29.11.2578-2586.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. van Soolingen D., Qian L., de Haas P. E., Douglas J. T., Traore H., Portaels F., Qing H. Z., Enkhsaikan D., Nymadawa P., van Embden J. D. Predominance of a single genotype of Mycobacterium tuberculosis in countries of east Asia. J Clin Microbiol. 1995 Dec;33(12):3234–3238. doi: 10.1128/jcm.33.12.3234-3238.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van Soolingen D., de Haas P. E., Haagsma J., Eger T., Hermans P. W., Ritacco V., Alito A., van Embden J. D. Use of various genetic markers in differentiation of Mycobacterium bovis strains from animals and humans and for studying epidemiology of bovine tuberculosis. J Clin Microbiol. 1994 Oct;32(10):2425–2433. doi: 10.1128/jcm.32.10.2425-2433.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

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