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. 1996 Sep;34(9):2106–2112. doi: 10.1128/jcm.34.9.2106-2112.1996

Candida albicans serotype analysis by flow cytometry.

S Mercure 1, S Sénéchal 1, P Auger 1, G Lemay 1, S Montplaisir 1
PMCID: PMC229198  PMID: 8862566

Abstract

Candida albicans strains can be assigned to either of two major serogroups, A or B. Antigenic surface determinants present only in serotype A strains allow such a distinction, which has epidemiologic relevance. Reports have established that the relative distributions of the two serotypes can vary depending on the geographic origin of the isolates. A prevalence of susceptibility to an antifungal agent, flucytosine, was also observed with isolates of serotype A. More recently, it was suggested that the occurrence of serotype B isolates in various clinical forms of candidiasis is increasing. However, this latest finding remains controversial since serotyping results vary widely from one laboratory to another because of the lack of standardized methodologies. Difficulty in interpretation of results, which may lead to erroneous serotype identification, is the major setback associated with current methods. For this study, we thus devised a procedure that relies on flow cytometry and that may eliminate ambiguities in serotype determination. The validation of results was achieved with two types of serotype A-specific antisera, Iatron Factor 6 antiserum and an anti-C. albicans antiserum adsorbed on serotype B yeast cells. Agreement between results obtained with these two reagents was 100% with a wide array of Candida strains. These results confirmed the potential of the flow cytometric procedure as a reliable and reproducible method to establish the serotypes of C. albicans strains. Furthermore, some applications of this procedure to the epidemiological study of this human pathogen are presented.

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Selected References

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  1. Auger P., Dumas C., Joly J. A study of 666 strains of Candida albicans: correlation between serotype and susceptibility to 5-fluorocytosine. J Infect Dis. 1979 May;139(5):590–594. doi: 10.1093/infdis/139.5.590. [DOI] [PubMed] [Google Scholar]
  2. Brawner D. L., Anderson G. L., Yuen K. Y. Serotype prevalence of Candida albicans from blood culture isolates. J Clin Microbiol. 1992 Jan;30(1):149–153. doi: 10.1128/jcm.30.1.149-153.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brawner D. L. Comparison between methods for serotyping of Candida albicans produces discrepancies in results. J Clin Microbiol. 1991 May;29(5):1020–1025. doi: 10.1128/jcm.29.5.1020-1025.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brawner D. L., Cutler J. E. Oral Candida albicans isolates from nonhospitalized normal carriers, immunocompetent hospitalized patients, and immunocompromised patients with or without acquired immunodeficiency syndrome. J Clin Microbiol. 1989 Jun;27(6):1335–1341. doi: 10.1128/jcm.27.6.1335-1341.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chaffin W. L., Ringler L., Larsen H. S. Interactions of monospecific antisera with cell surface determinants of Candida albicans. Infect Immun. 1988 Dec;56(12):3294–3296. doi: 10.1128/iai.56.12.3294-3296.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Coudert J., Kien Truong Thai, Ambroise-Thomas P., Douchet C., Pothier M. A. Septicémie a candida albicans. Etude sérologique par immuno-fluorescence, agglutination et immuno-électrophorèse. Bull Soc Pathol Exot Filiales. 1967 Nov-Dec;60(6):497–503. [PubMed] [Google Scholar]
  7. Drouhet E., Mercier-Soucy L., Montplaisir S. Sensibilité et résistance des levures pathogènes aux 5-fluoropyrimidines. I.--Relation entre les phénotypes de résistance a la 5-fluorocytosine, le sérotype de Candida albicans et l'écologie de différentes espèces de Candida d'origine humaine. Ann Microbiol (Paris) 1975 Jul-Aug;126B(1):25–39. [PubMed] [Google Scholar]
  8. Guinet R. M., Gabriel S. M. Candida albicans group A-specific soluble antigens demonstrated by quantitative immunoelectrophoresis. Infect Immun. 1980 Sep;29(3):853–858. doi: 10.1128/iai.29.3.853-858.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HASENCLEVER H. F., MITCHELL W. O. Antigenic studies of Candida. I. Observation of two antigenic groups in Candida albicans. J Bacteriol. 1961 Oct;82:570–573. doi: 10.1128/jb.82.4.570-573.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Imbert-Bernard C., Valentin A., Reynes J., Mallié M., Bastide J. M. Relationship between fluconazole sensitivity of Candida albicans isolates from HIV positive patients and serotype, adherence and CD4+ lymphocyte count. Eur J Clin Microbiol Infect Dis. 1994 Sep;13(9):711–716. doi: 10.1007/BF02276053. [DOI] [PubMed] [Google Scholar]
  11. Kobayashi H., Shibata N., Suzuki S. Evidence for oligomannosyl residues containing both beta-1,2 and alpha-1,2 linkages as a serotype A-specific epitope(s) in mannans of Candida albicans. Infect Immun. 1992 May;60(5):2106–2109. doi: 10.1128/iai.60.5.2106-2109.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mercure S., Montplaisir S., Lemay G. Correlation between the presence of a self-splicing intron in the 25S rDNA of C.albicans and strains susceptibility to 5-fluorocytosine. Nucleic Acids Res. 1993 Dec 25;21(25):6020–6027. doi: 10.1093/nar/21.25.6020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mercure S., Poirier S., Lemay G., Auger P., Montplaisir S., de Repentigny L. Application of biotyping and DNA typing of Candida albicans to the epidemiology of recurrent vulvovaginal candidiasis. J Infect Dis. 1993 Aug;168(2):502–507. doi: 10.1093/infdis/168.2.502. [DOI] [PubMed] [Google Scholar]
  14. Merz W. G. Candida albicans strain delineation. Clin Microbiol Rev. 1990 Oct;3(4):321–334. doi: 10.1128/cmr.3.4.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miyakawa Y., Kuribayashi T., Kagaya K., Suzuki M., Nakase T., Fukazawa Y. Role of specific determinants in mannan of Candida albicans serotype A in adherence to human buccal epithelial cells. Infect Immun. 1992 Jun;60(6):2493–2499. doi: 10.1128/iai.60.6.2493-2499.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Odds F. C., Abbott A. B. A simple system for the presumptive identification of Candida albicans and differentiation of strains within the species. Sabouraudia. 1980 Dec;18(4):301–317. [PubMed] [Google Scholar]
  17. Poulain D., Hopwood V., Vernes A. Antigenic variability of Candida albicans. Crit Rev Microbiol. 1985;12(3):223–270. doi: 10.3109/10408418509104430. [DOI] [PubMed] [Google Scholar]
  18. Reiss E., de Repentigny L., Kuykendall R. J., Carter A. W., Galindo R., Auger P., Bragg S. L., Kaufman L. Monoclonal antibodies against Candida tropicalis mannan: antigen detection by enzyme immunoassay and immunofluorescence. J Clin Microbiol. 1986 Nov;24(5):796–802. doi: 10.1128/jcm.24.5.796-802.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. St-Germain G., Dion C., Espinel-Ingroff A., Ratelle J., de Repentigny L. Ketoconazole and itraconazole susceptibility of Candida albicans isolated from patients infected with HIV. J Antimicrob Chemother. 1995 Jul;36(1):109–118. doi: 10.1093/jac/36.1.109. [DOI] [PubMed] [Google Scholar]
  20. Stiller R. L., Bennett J. E., Scholer H. J., Wall M., Polak A., Stevens D. A. Susceptibility to 5-fluorocytosine and prevalence of serotype in 402 Candida albicans isolates from the United States. Antimicrob Agents Chemother. 1982 Sep;22(3):482–487. doi: 10.1128/aac.22.3.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sullivan D. J., Westerneng T. J., Haynes K. A., Bennett D. E., Coleman D. C. Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals. Microbiology. 1995 Jul;141(Pt 7):1507–1521. doi: 10.1099/13500872-141-7-1507. [DOI] [PubMed] [Google Scholar]
  22. Suzuki M., Fukazawa Y. Immunochemical characterization of Candida albicans cell wall antigens: specific determinant of Candida albicans serotype A mannan. Microbiol Immunol. 1982;26(5):387–402. doi: 10.1111/j.1348-0421.1982.tb00189.x. [DOI] [PubMed] [Google Scholar]
  23. Tsuchiya T., Fukazawa Y., Taguchi M., Nakase T., Shinoda T. Serologic aspects on yeast classification. Mycopathol Mycol Appl. 1974 Aug 30;53(1):77–91. doi: 10.1007/BF02127199. [DOI] [PubMed] [Google Scholar]
  24. Velegraki A. In vitro susceptibility to itraconazole and fluconazole of switch phenotypes of Candida albicans serotypes A and B, isolated from immunocompromised hosts. J Med Vet Mycol. 1995 Jan-Feb;33(1):83–85. [PubMed] [Google Scholar]

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