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. 1996 Sep;34(9):2275–2279. doi: 10.1128/jcm.34.9.2275-2279.1996

Diagnosis of extrapulmonary tuberculosis by Gen-Probe amplified Mycobacterium tuberculosis direct test.

S Ehlers 1, R Ignatius 1, T Regnath 1, H Hahn 1
PMCID: PMC229231  PMID: 8862598

Abstract

A total of 294 specimens collected from nonrespiratory sites of 268 patients were tested for direct detection of Mycobacterium tuberculosis complex by the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test (AMTD). The specimens included ascitic, pleural, pericardial, and synovial fluids, abscess aspirates, and tissue and lymph node biopsy samples, as well as gastric aspirates and cerebrospinal fluid samples. All samples were processed by the N-acetyl-L-cysteine-sodium hydroxide decontamination procedure prior to testing. Twenty samples showed acid-fast bacilli on auramine staining, and 48 samples were positive by AMTD, 9 of which were negative for M. tuberculosis complex by culture. After reviewing the patients clinical charts to resolve discrepancies, the test result of one cerebrospinal fluid sample was considered to be false positive by AMTD. The overall sensitivity, specificity, positive predictive value, and negative predictive value were 83.9, 99.6, 97.9, and 96.3%, respectively. No significant differences were found when AMTD results obtained with specimens of nonrespiratory origin were compared with assay results obtained with samples of respiratory origin (P > 0.05). In conclusion, our results demonstrate that AMTD performs equally well with all types of specimens.

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Selected References

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  1. Abe C., Hirano K., Wada M., Kazumi Y., Takahashi M., Fukasawa Y., Yoshimura T., Miyagi C., Goto S. Detection of Mycobacterium tuberculosis in clinical specimens by polymerase chain reaction and Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. J Clin Microbiol. 1993 Dec;31(12):3270–3274. doi: 10.1128/jcm.31.12.3270-3274.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abe C., Hosojima S., Fukasawa Y., Kazumi Y., Takahashi M., Hirano K., Mori T. Comparison of MB-Check, BACTEC, and egg-based media for recovery of mycobacteria. J Clin Microbiol. 1992 Apr;30(4):878–881. doi: 10.1128/jcm.30.4.878-881.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beavis K. G., Lichty M. B., Jungkind D. L., Giger O. Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens. J Clin Microbiol. 1995 Oct;33(10):2582–2586. doi: 10.1128/jcm.33.10.2582-2586.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carpentier E., Drouillard B., Dailloux M., Moinard D., Vallee E., Dutilh B., Maugein J., Bergogne-Berezin E., Carbonnelle B. Diagnosis of tuberculosis by Amplicor Mycobacterium tuberculosis test: a multicenter study. J Clin Microbiol. 1995 Dec;33(12):3106–3110. doi: 10.1128/jcm.33.12.3106-3110.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. D'Amato R. F., Wallman A. A., Hochstein L. H., Colaninno P. M., Scardamaglia M., Ardila E., Ghouri M., Kim K., Patel R. C., Miller A. Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test. J Clin Microbiol. 1995 Jul;33(7):1832–1834. doi: 10.1128/jcm.33.7.1832-1834.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ehlers S., Pirmann M., Zaki W., Hahn H. Evaluation of a commercial rRNA target amplification assay for detection of Mycobacterium tuberculosis complex in respiratory specimens. Eur J Clin Microbiol Infect Dis. 1994 Oct;13(10):827–829. doi: 10.1007/BF02111344. [DOI] [PubMed] [Google Scholar]
  7. Isenberg H. D., D'Amato R. F., Heifets L., Murray P. R., Scardamaglia M., Jacobs M. C., Alperstein P., Niles A. Collaborative feasibility study of a biphasic system (Roche Septi-Chek AFB) for rapid detection and isolation of mycobacteria. J Clin Microbiol. 1991 Aug;29(8):1719–1722. doi: 10.1128/jcm.29.8.1719-1722.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jonas V., Alden M. J., Curry J. I., Kamisango K., Knott C. A., Lankford R., Wolfe J. M., Moore D. F. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA. J Clin Microbiol. 1993 Sep;31(9):2410–2416. doi: 10.1128/jcm.31.9.2410-2416.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KUBICA G. P., DYE W. E., COHN M. L., MIDDLEBROOK G. Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria. Am Rev Respir Dis. 1963 May;87:775–779. doi: 10.1164/arrd.1963.87.5.775. [DOI] [PubMed] [Google Scholar]
  10. Miller N., Hernandez S. G., Cleary T. J. Evaluation of Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and PCR for direct detection of Mycobacterium tuberculosis in clinical specimens. J Clin Microbiol. 1994 Feb;32(2):393–397. doi: 10.1128/jcm.32.2.393-397.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moore D. F., Curry J. I. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by Amplicor PCR. J Clin Microbiol. 1995 Oct;33(10):2686–2691. doi: 10.1128/jcm.33.10.2686-2691.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pfyffer G. E., Kissling P., Wirth R., Weber R. Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system. J Clin Microbiol. 1994 Apr;32(4):918–923. doi: 10.1128/jcm.32.4.918-923.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stauffer F., Mutschlechner R., Hasenberger P., Stadlbauer S., Schinko H. Detection of Mycobacterium tuberculosis complex in clinical specimens by a commercial polymerase chain reaction kit. Eur J Clin Microbiol Infect Dis. 1995 Dec;14(12):1046–1051. doi: 10.1007/BF01590937. [DOI] [PubMed] [Google Scholar]
  14. Vlaspolder F., Singer P., Roggeveen C. Diagnostic value of an amplification method (Gen-Probe) compared with that of culture for diagnosis of tuberculosis. J Clin Microbiol. 1995 Oct;33(10):2699–2703. doi: 10.1128/jcm.33.10.2699-2703.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vuorinen P., Miettinen A., Vuento R., Hällström O. Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and Roche Amplicor Mycobacterium Tuberculosis Test. J Clin Microbiol. 1995 Jul;33(7):1856–1859. doi: 10.1128/jcm.33.7.1856-1859.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

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