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. 1996 Sep;34(9):2312–2315. doi: 10.1128/jcm.34.9.2312-2315.1996

Evaluation of an infectivity standard for real-time quality control of human immunodeficiency virus type 1 quantitative micrococulture assays. Participating Laboratories of The AIDS Clinical Trials Group.

W A Scott 1, D Brambilla 1, E Siwak 1, C Beatty 1, J Bremer 1, R W Coombs 1, H Farzadegan 1, S A Fiscus 1, S M Hammer 1, F B Hollinger 1, N Khan 1, S Rasheed 1, P S Reichelderfer 1
PMCID: PMC229242  PMID: 8862609

Abstract

Quantitative microculture assays of cryopreserved human immunodeficiency virus type 1-infected cell suspensions and culture supernatants were compared among seven assays sites. There was no significant change in titer during 1 year of storage. The overall standard deviation for infected cell suspensions was approximately 0.8 log10 virus titer. A method for detecting deviant assay results was developed and was used to identify two donor cell preparations (n = 54) that gave consistently low titers.

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Selected References

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