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. 1996 Sep;34(9):2331–2333. doi: 10.1128/jcm.34.9.2331-2333.1996

Multiplex PCR provides a low-cost alternative to DNA probe methods for rapid identification of Mycobacterium avium and Mycobacterium intracellulare.

D Cousins 1, B Francis 1, D Dawson 1
PMCID: PMC229248  PMID: 8862615

Abstract

A multiplex PCR designed to differentiate Mycobacterium tuberculosis complex organisms from M. avium and M. intracellulare was used to test 105 isolates identified by DNA probe methods as M. avium, M. intracellulare, or M. avium complex type X. The multiple PCR correctly identified 33 of 34 isolates identified by commercial probe methods as M. avium and all 51 isolates identified as M. intracellulare. The 20 isolates identified as M. avium complex type X by probe were identified as Mycobacterium spp. by the multiplex method. These results confirm that the multiplex PCR, which is simple to perform and cheaper than commercial probe methods, is suitable for routine identification of M. avium and M. intracellulare.

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Selected References

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  1. Butler W. R., Kilburn J. O. Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids. J Clin Microbiol. 1988 Jan;26(1):50–53. doi: 10.1128/jcm.26.1.50-53.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Böddinghaus B., Rogall T., Flohr T., Blöcker H., Böttger E. C. Detection and identification of mycobacteria by amplification of rRNA. J Clin Microbiol. 1990 Aug;28(8):1751–1759. doi: 10.1128/jcm.28.8.1751-1759.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cregan P., Yajko D. M., Ng V. L., Gonzalez P. C., Nassos P. S., Sanders C. A., Hadley W. K. Use of DNA probes to detect Mycobacterium intracellulare and "X" mycobacteria among clinical isolates of Mycobacterium avium complex. J Infect Dis. 1992 Jul;166(1):191–194. doi: 10.1093/infdis/166.1.191. [DOI] [PubMed] [Google Scholar]
  4. Evans K. D., Nakasone A. S., Sutherland P. A., de la Maza L. M., Peterson E. M. Identification of Mycobacterium tuberculosis and Mycobacterium avium-M. intracellulare directly from primary BACTEC cultures by using acridinium-ester-labeled DNA probes. J Clin Microbiol. 1992 Sep;30(9):2427–2431. doi: 10.1128/jcm.30.9.2427-2431.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Floyd M. M., Silcox V. A., Jones W. D., Jr, Butler W. R., Kilburn J. O. Separation of Mycobacterium bovis BCG from Mycobacterium tuberculosis and Mycobacterium bovis by using high-performance liquid chromatography of mycolic acids. J Clin Microbiol. 1992 May;30(5):1327–1330. doi: 10.1128/jcm.30.5.1327-1330.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hughes M. S., Skuce R. A., Beck L. A., Neill S. D. Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences. J Clin Microbiol. 1993 Dec;31(12):3216–3222. doi: 10.1128/jcm.31.12.3216-3222.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Inderlied C. B., Kemper C. A., Bermudez L. E. The Mycobacterium avium complex. Clin Microbiol Rev. 1993 Jul;6(3):266–310. doi: 10.1128/cmr.6.3.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Böttger E. C. Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol. 1993 Nov;31(11):2882–2889. doi: 10.1128/jcm.31.11.2882-2889.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Liébana E., Aranaz A., Francis B., Cousins D. Assessment of genetic markers for species differentiation within the Mycobacterium tuberculosis complex. J Clin Microbiol. 1996 Apr;34(4):933–938. doi: 10.1128/jcm.34.4.933-938.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Soini H., Eerola E., Viljanen M. K. Genetic diversity among Mycobacterium avium complex AccuProbe-positive isolates. J Clin Microbiol. 1996 Jan;34(1):55–57. doi: 10.1128/jcm.34.1.55-57.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol. 1993 Feb;31(2):175–178. doi: 10.1128/jcm.31.2.175-178.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thibert L., Lapierre S. Routine application of high-performance liquid chromatography for identification of mycobacteria. J Clin Microbiol. 1993 Jul;31(7):1759–1763. doi: 10.1128/jcm.31.7.1759-1763.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Viljanen M. K., Olkkonen L., Katila M. L. Conventional identification characteristics, mycolate and fatty acid composition, and clinical significance of MAIX AccuProbe-positive isolates of Mycobacterium avium complex. J Clin Microbiol. 1993 May;31(5):1376–1378. doi: 10.1128/jcm.31.5.1376-1378.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wayne L. G., Sramek H. A. Agents of newly recognized or infrequently encountered mycobacterial diseases. Clin Microbiol Rev. 1992 Jan;5(1):1–25. doi: 10.1128/cmr.5.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wilton S., Cousins D. Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube. PCR Methods Appl. 1992 May;1(4):269–273. doi: 10.1101/gr.1.4.269. [DOI] [PubMed] [Google Scholar]

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